David Bourget (Western Ontario)
David Chalmers (ANU, NYU)
Rafael De Clercq
Ezio Di Nucci
Jack Alan Reynolds
Learn more about PhilPapers
Daniel Gibson, Benders G., A. Gwynedd, Cynthia Andrews-Pfannkoch, Evgeniya Denisova, Baden-Tillson A., Zaveri Holly, Stockwell Jayshree, B. Timothy, Anushka Brownley, David Thomas, Algire W., A. Mikkel, Chuck Merryman, Lei Young, Vladimir Noskov, Glass N., I. John, J. Craig Venter, Clyde Hutchison, Smith A. & O. Hamilton
Science 319 (5867):1215--1220 (2008)
We have synthesized a 582,970-base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted "watermarks" at intergenic sites known to tolerate transposon insertions. Overlapping "cassettes" of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate assemblies of approximately 24 kb, 72 kb ("1/8 genome"), and 144 kb ("1/4 genome"), which were all cloned as bacterial artificial chromosomes in Escherichia coli. Most of these intermediate clones were sequenced, and clones of all four 1/4 genomes with the correct sequence were identified. The complete synthetic genome was assembled by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae, then isolated and sequenced. A clone with the correct sequence was identified. The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments. 10.1126/science.1151721.
|Categories||categorize this paper)|
Setup an account with your affiliations in order to access resources via your University's proxy server
Configure custom proxy (use this if your affiliation does not provide a proxy)
|Through your library|
References found in this work BETA
No references found.
Citations of this work BETA
Christophe Malaterre (2013). Synthetic Biology and Synthetic Knowledge. Biological Theory (8):346–356.
Bernd Giese, Stefan Koenigstein, Henning Wigger, Jan C. Schmidt & Arnim von Gleich (2013). Rational Engineering Principles in Synthetic Biology: A Framework for Quantitative Analysis and an Initial Assessment. Biological Theory 8 (4):324-333.
Randal A. Koene (2012). Experimental Research in Whole Brain Emulation: The Need for Innovativein Vivomeasurement Techniques. International Journal of Machine Consciousness 4 (01):35-65.
Leon Huynen, Craig D. Millar & David M. Lambert (2012). Resurrecting Ancient Animal Genomes: The Extinct Moa and More. Bioessays 34 (8):661-669.
John Basl & Ronald Sandler (2013). The Good of Non-Sentient Entities: Organisms, Artifacts, and Synthetic Biology. Studies in History and Philosophy of Science Part C: Studies in History and Philosophy of Biological and Biomedical Sciences 44 (4):697-705.
Similar books and articles
John Dupré (2004). Understanding Contemporary Genomics. Perspectives on Science 12 (3):320-338.
Ehud Lamm (2010). Genes Versus Genomes: The Role of Genome Organization in Evolution. Dissertation, Tel Aviv University
Ehud Lamm (2011). The Metastable Genome: A Lamarckian Organ in a Darwinian World? In Eva Jablonka & Snait Gissis (eds.), Transformations of Lamarckism: from subtle fluids to molecular biology. MIT Press
Alex Rosenberg (1994). Subversive Reflections on the Human Genome Project. PSA: Proceedings of the Biennial Meeting of the Philosophy of Science Association 1994:329 - 335.
Robin O. Andreasen & Milo J. Aukerman (2002). The Human Genome Project: A Reply to Rosenberg. [REVIEW] Biology and Philosophy 17 (5):673-678.
Christian Byk (1998). A Map to a New Treasure Island: The Human Genome and the Concept of Common Heritage. Journal of Medicine and Philosophy 23 (3):234 – 246.
Alexander E. Vinogradov (2001). Mirrored Genome Size Distributions in Monocot and Dicot Plants. Acta Biotheoretica 49 (1):43-51.
Ehud Lamm (2010). Review Of: Julian Huxley, Evolution: The Modern Synthesis – The Definitive Edition. [REVIEW] Integrative Psychological and Behavioral Science.
Valery Kirzhner, Eviatar Nevo, Abraham Korol & Alexander Bolshoy (2003). A Large-Scale Comparison of Genomic Sequences: One Promising Approach. Acta Biotheoretica 51 (2):73-89.
Amy Gutmann (2011). The Ethics of Synthetic Biology: Guiding Principles for Emerging Technologies. Hastings Center Report 41 (4):17-22.
Added to index2009-12-08
Total downloads12 ( #271,590 of 1,789,998 )
Recent downloads (6 months)1 ( #424,764 of 1,789,998 )
How can I increase my downloads?