David Bourget (Western Ontario)
David Chalmers (ANU, NYU)
Rafael De Clercq
Ezio Di Nucci
Jack Alan Reynolds
Learn more about PhilPapers
We present evidence of a potentially serious source of error intrinsic to all spotted cDNA microarrays that use IMAGE clones of expressed sequence tags (ESTs). We found that a high proportion of these EST sequences contain 5V-end poly(dT) sequences that are remnants from the oligo(dT)-primed reverse transcription of polyadenylated mRNA templates used to generate EST cDNA for sequence clone libraries. Analysis of expression data from two single-dye cDNA microarray experiments showed that ESTs whose sequences contain repeats of consecutive 5V- end dT residues appeared to be strongly coexpressed, while expression data of all other sequences exhibited no such pattern. Our analysis suggests that expression data from sequences containing 5V poly(dT) tracts are more likely to be due to systematic cross-hybridization of these poly(dT) tracts than to true mRNA coexpression. This indicates that existing data generated by cDNA microarrays containing IMAGE clone ESTs should be filtered to remove expression data containing significant 5V poly(dT) tracts. D 2003 Elsevier Inc. All rights reserved.
|Keywords||No keywords specified (fix it)|
No categories specified
(categorize this paper)
Setup an account with your affiliations in order to access resources via your University's proxy server
Configure custom proxy (use this if your affiliation does not provide a proxy)
|Through your library||
References found in this work BETA
No references found.
Citations of this work BETA
No citations found.
Similar books and articles
Paul Corazza (1999). Laver Sequences for Extendible and Super-Almost-Huge Cardinals. Journal of Symbolic Logic 64 (3):963-983.
David Danks, Clark Glymour & Peter Spirtes (2003). The Computational and Experimental Complexity of Gene Perturbations for Regulatory Network Search. In W. H. Hsu, R. Joehanes & C. D. Page (eds.), Proceedings of IJCAI-2003 workshop on learning graphical models for computational genomics.
C. P. Schnorr & P. Fuchs (1977). General Random Sequences and Learnable Sequences. Journal of Symbolic Logic 42 (3):329-340.
Tianjaio Chu, Two Statistical Problems for Inference to Regulatory Structure From Associations of Gene Expression Measurements with Microarrays.
V. Michele Abrusci, Jean-Yves Girard & Jacques Van De Wiele (1990). Some Uses of Dilators in Combinatorial Problems. II. Journal of Symbolic Logic 55 (1):32-40.
Clark Glymour, Two Statistical Problems for Inference to Regulatory Structure From Associations of Gene Expression Measurements with Microarrays.
Joan Rand Moschovakis (1987). Relative Lawlessness in Intuitionistic Analysis. Journal of Symbolic Logic 52 (1):68-88.
Added to index2009-01-28
Total downloads25 ( #155,112 of 1,907,534 )
Recent downloads (6 months)10 ( #66,877 of 1,907,534 )
How can I increase my downloads?