Dynamic functional and structural analysis of living cells: New tools for vital staining of nuclear DNA and for characterisation of cell motion
David Bourget (Western Ontario)
David Chalmers (ANU, NYU)
Rafael De Clercq
Jack Alan Reynolds
Learn more about PhilPapers
Acta Biotheoretica 43 (4):299-317 (1995)
Increasing interest has been paid to applications of fluorescence measurements to analyze physiological mechanisms in living cells. However, few studies have taken advantage of DNA quantification by fluorometry for dynamic assessment of chromatin organization as well as cell motion during the cell cycle. This approach involves both optimal conditions for DNA staining and cell tracking methods. In this context, this report describes a stoichiometric method for nuclear DNA specific staining, using the bisbenzimidazole dye Hoechst 33342 associated with verapamil, a calcium membrane channel blocker. This method makes it possible to correlate variations of nuclear DNA content with cell motion in cells that are maintained alive. Motion measurement is the second goal of this paper and it explains the snake-spline method, and the associated cell following method.
|Keywords||No keywords specified (fix it)|
|Categories||categorize this paper)|
Setup an account with your affiliations in order to access resources via your University's proxy server
Configure custom proxy (use this if your affiliation does not provide a proxy)
|Through your library|
References found in this work BETA
No references found.
Citations of this work BETA
No citations found.
Similar books and articles
Melinda B. Fagan (2011). Social Experiments in Stem Cell Biology. Perspectives on Science 19 (3):235-262.
Mark T. Brown (2009). Moral Complicity in Induced Pluripotent Stem Cell Research. Kennedy Institute of Ethics Journal 19 (1):pp. 1-22.
Philippe Tracqui & Jacques Ohayon (2004). Transmission of Mechanical Stresses Within the Cytoskeleton of Adherent Cells: A Theoretical Analysis Based on a Multi-Component Cell Model. Acta Biotheoretica 52 (4):323-341.
Christine Reder (2001). Mutated mtDNA Distribution in Exponentially Growing Cell Cultures and How the Segregation Rate is Increased by the Mitochondrial Compartments. Acta Biotheoretica 49 (4):235-245.
Jürgen Bereiter-Hahn (1985). Architecture of Tissue Cells the Structural Basis Which Determines Shape and Locomotion of Cells. Acta Biotheoretica 34 (2-4):139-148.
W. Schwemmler (1982). The Endoeytobiotic Cell Theory and the Periodic System of Cells. Acta Biotheoretica 31 (1):45-68.
Rosine Chandebois & Jacob Faber (1987). From DNA Transcription to Visible Structure: What the Development of Multicellular Animals Teaches Us. Acta Biotheoretica 36 (2):61-119.
Joachim W. Ellwart & Peter Dörmer (1992). Measurement of Efflux From G1-Phase in a Growth Factor Dependent Cell Line. Acta Biotheoretica 40 (2-3):139-146.
José Pierrez & Xavier Ronot (1992). Flow Cytometric Analysis of the Cell Cycle: Mathematical Modeling and Biological Interpretation. Acta Biotheoretica 40 (2-3):131-137.
Added to index2009-01-28
Total downloads5 ( #270,402 of 1,693,218 )
Recent downloads (6 months)1 ( #209,787 of 1,693,218 )
How can I increase my downloads?