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Modern physics asks: how do the objects exist? This kind of question inevitably touches upon philosophy; to be precise, it involves metaphysics that traditionally deals with these problems. There are grounds to assume that a quantum object in a certain sense does not exist until it is registered. Thus, one of the conclusions says, “Photon is a photon if it is a registered photon”. This is a paraphrase of well-known Wheeler’s words about the essence of quantum phenomenon. These effects cannot (...) be understood if we come from the assumption that all existing (‘real’) and quantum objects in particular, is only being of the actual. To explain quantum mechanics phenomena we need to realize that there exists another modus of being. Quantum mechanics refers to some sort oftranscendence. Such conclusion is based not only on the analysis of the testing of Aspeckt’s experiments. The whole structure of quantum mechanics confirms it. The revision of the New European paradigm leads to the return of such conception of existence that brings us to the traditional metaphysical understanding of the being and implies its study on several levels of existence. The conclusion of this kind leads to a serious correction of the philosophical model of the world built by modern natural science. (shrink)
We have synthesized a 582,970-base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted "watermarks" at intergenic sites known to tolerate transposon insertions. Overlapping "cassettes" of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate (...) assemblies of approximately 24 kb, 72 kb ("1/8 genome"), and 144 kb ("1/4 genome"), which were all cloned as bacterial artificial chromosomes in Escherichia coli. Most of these intermediate clones were sequenced, and clones of all four 1/4 genomes with the correct sequence were identified. The complete synthetic genome was assembled by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae, then isolated and sequenced. A clone with the correct sequence was identified. The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments. 10.1126/science.1151721. (shrink)
