We have synthesized a 582,970-base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted "watermarks" at intergenic sites known to tolerate transposon insertions. Overlapping "cassettes" of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate (...) assemblies of approximately 24 kb, 72 kb ("1/8 genome"), and 144 kb ("1/4 genome"), which were all cloned as bacterial artificial chromosomes in Escherichia coli. Most of these intermediate clones were sequenced, and clones of all four 1/4 genomes with the correct sequence were identified. The complete synthetic genome was assembled by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae, then isolated and sequenced. A clone with the correct sequence was identified. The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments. 10.1126/science.1151721. (shrink)
Erotikon brings together leading contemporary intellectuals from a variety of fields for an expansive debate on the full meaning of eros . Renowned scholars of philosophy, literature, classics, psychoanalysis, theology, and art history join poets and a novelist to offer fresh insights into a topic that is at once ancient and forever young. Restricted neither by historical period nor by genre, these contributions explore manifestations of eros throughout Western culture, in subjects ranging from ancient philosophy and baroque architecture to modern (...) literature and Hollywood cinema. An idea charged with paradox, eros has always defied categorization, and yet it cannot--it will not--be ignored. Erotikon aims to raise the difficult question of what, if anything, unifies the erotic manifold. How is eros in a sculpture like eros in a poem? Does the ancient story of Cupid and Psyche still speak meaningfully to modern readers, and if so, why? Is Plato's eros the same as Freud's? Or Proust's? And what is the erotic dimension in Nietzsche's thought? While each essay takes on a specific issue, together they constitute a wide-ranging conversation in which these broader questions are at play. A compilation of the latest, best efforts to reckon with eros , Erotikon will appeal not just to scholars and educators, but also to artists and critics, to the curious and the disillusioned, to the prurient and the prudent. Contributors: Shadi Bartsch Peter Brooks J. M. Coetzee Catharine Edwards Anthony Grafton Tom Gunning David M. Halperin Valentina Izmirlieva Jonathan Lear Eric Marty Susan Mitchell Glenn W. Most Martha C. Nussbaum Robert B. Pippin James I. Porter Philippe Roger Ingrid D. Rowland Eric L. Santner Mark Strand David Tracy Richard Wollheim Slavoj Zizek. (shrink)
J. Schumpeter is a key figure, even a seminal one, on technological innovation. Most economists who study technological innovation refer to Schumpeter and his pioneering role in introducing innovation into economic studies. However, despite having brought forth the concept of innovation in economic theory, Schumpeter provided few if any analyses of the process of innovation itself. This paper suggests that the origin of systematic studies on technological innovation owes its existence to the economist W. Rupert Maclaurin from MIT. In (...) the 1940s and 1950s, Maclaurin developed Schumpeter’s ideas, analyzing technological innovation as a process composed of several stages or steps, and proposed a theory of technological innovation, later called the linear model of innovation. The paper also argues that Maclaurin constructed one of the first taxonomies for measuring technological innovation. (shrink)
[Allen W. Wood] Kant's moral philosophy is grounded on the dignity of humanity as its sole fundamental value, and involves the claim that human beings are to be regarded as the ultimate end of nature. It might be thought that a theory of this kind would be incapable of grounding any conception of our relation to other living things or to the natural world which would value nonhuman creatures or respect humanity's natural environment. This paper criticizes Kant's argumentative strategy for (...) dealing with our duties in regard to animals, but defends both his theory and most of his conclusions on these topics. /// [Onora O'Neill] Kant's ethics, like others, has unavoidable anthropocentric starting points: only humans, or other 'rational natures', can hold obligations. Seemingly this should not make speciesist conclusions unavoidable: might not rational natures have obligations to the non-rational? However, Kant's argument for the unconditional value of rational natures cannot readily be extended to show that all non-human animals have unconditional value, or rights. Nevertheless Kant's speciesism is not thoroughgoing. He does not view non-rational animals as mere items for use. He allows for indirect duties 'with regard to' them which afford welfare but not rights, and can allow for indirect duties 'with regard to' abstract and dispersed aspects of nature, such as biodiversity, species and habitats. (shrink)
Lang, B. Philosophy and the manners of art.--Hofstadter, A. Freedom, enownment, and philosophy.--Mehta, J. L. A stranger from Asia.--Fox, D. A. A passage past India.--Rucker, D. Philosophy and the constitution of Emerson's world.--Schneider, H. W. The pragmatic movement in historical perspective.--Barnes, H. E. Reflections on myth and magic.--Cauvel, J. The imperious presence of theater.--Seay, A. Musical conservatism in the fourteenth century.--Hochman, W. R. The enduring fascination of war.--Davenport, M. M. J. Glenn Gray and the promise of wisdom.