The stories in this book offer strategies and practices for applying systems thinking in education to unleash human energy for the journey of continuous improvement.

Regeneration capabilities are found in most or all animals. Whether regeneration is part of the development of an animal or a distinct phenomenon independent of development is a debatable question. If we consider regeneration as a process belonging to development, similarly to embryogenesis or metamorphosis, the existence of regenerative capabilities in adults can be seen as an argument in favor of the theory that development continues throughout the life of animals. Here I perform a comparative analysis of (...) regeneration versus “classical” developmental processes in animals in order to determine to what extent these processes are inclusive or distinct. I identify the existence of regeneration-specific processes, i.e., processes that occur during the regeneration, but not the initial development, of a given structure. In addition, I find that seemingly similar processes acting during development and regeneration may have differential molecular and cellular bases. I thus conclude that there are significant differences between regeneration processes in adult animals and developmental processes occurring during earlier phases of the life cycle. The existence of regenerative capabilities in adult animals can therefore not be used as an argument in favor of the idea that development spans the whole life. (shrink)

• Aggregates of previously isolated cells of Hydra are capable, under suitable solvant conditions, of regeneration forming complete animals. In a first stage, ecto- and endodermal cells sort out, producing the bilayered hollow structure characteristic of Hydra tissue; thereafter, heads are formed (even if the original cell preparation contained no head cells), eventually leading to the separation of normal animals with head, body column and foot. Hydra appears to be the highest type of organism that allows for regeneration (...) of the entire structure from random cell aggregates. The system is particularly useful for studying cell interactions, tissue polarity, pattern formation, and cell differentiation. (shrink)

An emerging concept is that quiescent mature skeletal cells provide an important cellular source for bone regeneration. It has long been considered that a small number of resident skeletal stem cells are solely responsible for the remarkable regenerative capacity of adult bones. However, recent in vivo lineage‐tracing studies suggest that all stages of skeletal lineage cells, including dormant pre‐adipocyte‐like stromal cells in the marrow, osteoblast precursor cells on the bone surface and other stem and progenitor cells, are concomitantly recruited (...) to the injury site and collectively participate in regeneration of the damaged skeletal structure. Lineage plasticity appears to play an important role in this process, by which mature skeletal cells can transform their identities into skeletal stem cell‐like cells in response to injury. These highly malleable, long‐living mature skeletal cells, readily available throughout postnatal life, might represent an ideal cellular resource that can be exploited for regenerative medicine. (shrink)

The convergence of morphogenesis into viable organisms under variable conditions suggests closed‐loop dynamics involving multiscale functional feedback. We develop the idea that morphogenesis is based on synergy between mechanical and bio‐signaling processes, spanning all levels of organization: molecular, cellular, tissue, up to the whole organism. This synergy provides feedback within and between all levels of organization, to close the loop between the dynamics of the morphogenesis process and its robust functional outcome. Hydra offer a powerful platform to explore this direction, (...) thanks to their simple body plan, extraordinary regeneration capabilities, and the accessibility and flexibility of their tissues. Our recent experiments show that structural inheritance of the actomyosin organization directs body‐axis formation during Hydra regeneration. Morphogenesis is then stabilized through dynamic cytoskeletal reorganization induced by the inherited structure. The observed cytoskeletal stability and reorganization capabilities suggest that mechanical feedback integrates with biochemical processes to establish viable patterns and ensure canalization. (shrink)

The African neo-Pentecostal teaching that Christians continue to suffer from generational curses or bloodline curses is analysed from the perspective of Christian salvation as spiritual recreation. The main question considered in this article is: Soteriologically, how may we evaluate the ANP view that ‘born again’ Christians remain vulnerable to generational curses? The article describes the ANP assertion that Christians live under the threat of generational curses. Furthermore, the ANP’s understanding of the nature of generational curses is examined. Attention is further (...) given to the ANP’s view of the role played by a person’s blood in the transmission of generational curses and how this makes Christians vulnerable to generational curses after their conversion. The article also analyses how the doctrine of generational curses expresses a notion of Christian salvation with limited creative powers in Africa. Lastly, some elements that should inform an understanding of Christian salvation when addressing the fear of generational curses among the ANPs are proposed. The significance of this article lies in contributing a theological analysis that will help African Christians find confidence in their Christian salvation.Contribution: The article attempts to contribute towards the formulation of a biblically informed systematic theological framework of analysing and critiquing the logic of salvation in the ANP teaching that generational curses remain on regenerated Christians. (shrink)

Here we review and discuss the link between regeneration capacity and tumor suppression comparing mammals (embryos versus adults) with highly regenerative vertebrates. Similar to mammal embryo morphogenesis, in amphibians (essentially newts and salamanders) the reparative process relies on a precise molecular and cellular machinery capable of sensing abnormal signals and actively reprograming or eliminating them. As the embryo's evil twin, tumor also retains common functional attributes. The immune system plays a pivotal role in maintaining a physiological balance to provide (...) surveillance against tumor initiation or to support its initiation and progression. We speculate that susceptibility to cancer development in adult mammals may be determined by the loss of an advanced regenerative capability during evolution and believe that gaining mechanistic insights into how regenerative capacity linked to tumor suppression is postnatally lost in mammals might illuminate an as yet unrecognized route to cancer treatment. (shrink)

Early in his career Thomas Hunt Morgan was interested in embryology and dedicated his research to studying organisms that could regenerate. Widely regarded as a regeneration expert, Morgan was invited to deliver a series of lectures on the topic that he developed into a book, Regeneration. In addition to presenting experimental work that he had conducted and supervised, Morgan also synthesized and critiqued a great deal of work by his peers and predecessors. This essay probes into the history (...) of regeneration studies by looking in depth at Regeneration and evaluating Morgan’s contribution. Although famous for his work with fruit fly genetics, studying Regeneration illuminates Morgan’s earlier scientific approach which emphasized the importance of studying a diversity of organisms. Surveying a broad range of regenerative phenomena allowed Morgan to institute a standard scientific terminology that continues to inform regeneration studies today. Most importantly, Morgan argued that regeneration was a fundamental aspect of the growth process and therefore should be accounted for within developmental theory. Establishing important similarities between regeneration and development allowed Morgan to make the case that regeneration could act as a model of development. The nature of the relationship between embryogenesis and regeneration remains an active area of research. (shrink)

Here we endorse Hull’s replicator/interactor framework as providing the overarching understanding sought by MacCord and Maienschein. We suggest that difficulties in seeing the regeneration of limbs by salamanders and of forest ecosystems after fires as similar evolutionary processes can be overcome in this framework. In generalizing Dawkins’s “selfish gene” perspective, Hull defined natural selection as “a process in which the differential extinction and proliferation of interactors causes the differential perpetuation of the replicators that produced them”. Although genes and bacteria (...) are simultaneously both replicators and interactors, communities and ecosystems are generally only interactors. As reproducers, members of sexual species are intermediate. Within such species, organisms are indeed the interactors whose “differential extinction and proliferation” causes the “differential perpetuation” of replicators. But sexually-reproducing organisms do not individually replicate, persist, or recur as interactors, and in consequence it is only those genes causing an interactor differential that are specifically perpetuated in the long run. Higher-level interactors may seldom if ever reproduce, but their recurrence does enable the “differential perpetuation” of replicators specifically responsible for their “differential extinction and proliferation”. In offering answers to the question “What are the replicators specifically responsible for the differential extinction and proliferation of such higher-level entities?” we hope to unify adaptive regeneration across scales, from organisms to ecosystems. (shrink)

Diverse living systems possess the capacity for regeneration; that is, they can under some circumstances repair, re-produce, and maintain themselves in the face of disturbance or damage. Think of systems as diverse as forests, microbial biofilms, corals, salamanders, hydra, and human skin cells. This capacity is fundamental to life—without it, many biological systems would be too fragile to cope with stress and would frequently collapse—but because it is multiply realized in wildly different living systems at many scales, finding a (...) common understanding may seem futile, and in fact research has proceeded along independent lines. For example, the sciences of organismal regeneration and ecological regeneration appear to have little more than a nominal relationship. Progress towards unification can be made, we think, by turning to evolutionary theory, specifically by synthesizing recent discussions of the evolution of individuals versus collectives with older literature about replicators versus interactors. This allows us to provide a partial answer in response to MacCord and Maienschein’s call for “basic units and mechanisms” of regeneration, relevant when that process is “adaptive.” We argue that the basics of adaptive regeneration can be understood and generalized through a modification of David Hull’s replicator-interactor framework. (shrink)

Posthumanist and New Materialist thought attempts to undo the supremacy and distinction of the human being through accounting for the agential capacities of the animal and material world. New Materialism in particular constructs a vision of a vital natural world in order to turn us away from humanism and toward a more holistic understanding of nature, and political actants. In this article, I argue that there can be a humanist new materialist position that sees the vitalism of the natural world (...) while maintaining the uniqueness of the human being. This position can be found in the natural science of the eighteenth century in the work of Georges-Louis LeClerc de Buffon and Charles Bonnet. Reminding ourselves of this alternative mode of thinking about a vital nature, and its vision of a self-regenerating human being in a vital and regenerative universe, is a path toward regenerating humanism itself in contemporary scholarship. (shrink)

In this paper I argue that the value attributed to coral reefs drives the characterisation of evidence for their regeneration or degradation. I observe that regeneration and degradation depend on an understanding of what an ecosystem looks like when undegraded (a baseline), and that many mutually exclusive baselines can be given for any single case. Consequently, facts about ecological processes are insufficient to usefully and non-arbitrarily characterise changes to ecosystems. By examining how baselines and the value of reefs (...) interact in coral and algal reef examples, I argue that considering the value of an ecosystem is a necessity when describing processes like regeneration and degradation. This connects value as studied in socio-ecological and economic research with values as discussed in the philosophy of science literature. It also explains why such a broad range of processes may be considered regenerative, including those which introduce significant novelty, as well as pointing towards ways to mediate related debates, such as those surrounding novel and ‘pristine’ ecosystems. (shrink)

It is posited that the initiating event of amphibian regeneration of a limb, is retrodifferentiation* of what are to become the developing cells of the blastema. These cells reiterate a larval or premetamorphic ontogenic repertoire, induced by elevated levels of prolactin with adequate innervation. Subsequent redifferentiation of the blastema cells occurs, controlled by thyroxine and innervation.This temporal displacement of cellular morphologic characters in regeneration should be looked upon as a function of the ability to reiterate larval characters and (...) subsequently metamorphose. If correct, this would explain why amphibians which metamorphose only once, lose the ability to postmetamorphically regenerate. An exception to this,Xenopus laevis, an anuran which can epimorphically regenerate, to some extent, will be discussed.[/p]. (shrink)

This article is an expanded commentary on the essay “The Social Life of ‘Scaffolds’: Examining Human Rights in Regenerative Medicine.” In discussing the limits and possibilities of the essay, this commentary suggests that problematizing scaffolds in regenerative medicine as a kind of infrastructure rather than prosthetic opens the way for an understanding of the genesis of regenerative assemblages in ways that help to reframe inherent issues of human rights. Ultimately, it proposes the notion of experimental ecologies as a way of (...) thinking about an ethically driven productive entanglement of bodies, environments, and technology. (shrink)

Justin Barrett and Kelly James Clark have suggested that cognitive science of religion supports the existence of a god-faculty akin to sensus divinitatis. They propose that God may have given rise to the god-faculty via guided evolution. This suggestion raises two theological worries. First, our natural cognition seems to favor false god-beliefs over true ones. Second, it also makes us prone to tribalism. If God hates idolatry and moral evil, why would he give rise to mind with such biases? A (...) Plantingian response would point to the noetic effects of sin. Such a response, however, would have to assume that God is restoring the minds of believers. This paper considers empirical reasons to doubt that such a process is taking place. (shrink)

Early in his career Thomas Hunt Morgan was interested in embryology and dedicated his research to studying organisms that could regenerate. Widely regarded as a regeneration expert, Morgan was invited to deliver a series of lectures on the topic that he developed into a book, Regeneration (1901). In addition to presenting experimental work that he had conducted and supervised, Morgan also synthesized and critiqued a great deal of work by his peers and predecessors. This essay probes into the (...) history of regeneration studies by looking in depth at Regeneration and evaluating Morgan's contribution. Although famous for his work with fruit fly genetics, studying Regeneration illuminates Morgan's earlier scientific approach which emphasized the importance of studying a diversity of organisms. Surveying a broad range of regenerative phenomena allowed Morgan to institute a standard scientific terminology that continues to inform regeneration studies today. Most importantly, Morgan argued that regeneration was a fundamental aspect of the growth process and therefore should be accounted for within developmental theory. Establishing important similarities between regeneration and development allowed Morgan to make the case that regeneration could act as a model of development. The nature of the relationship between embryogenesis and regeneration remains an active area of research. (shrink)

Apprenticeship has always played both a social and economic role. Today, it forms part of the regeneration strategies of cities in the United Kingdom. This involves the creation and management of complex institutional relationships across the public and private domains of the civic landscape. This paper argues that it is through closely observed analysis of these meso-level developments (in contrast to studies of national systems) that we can reveal how the sustainability of vocational education and training initiatives depends on (...) the generation of civic social capital in the pursuit of collective goals. At the same time, the path-dependent nature of the clustering of social and economic inequality in urban post-industrial settings remains a constant reminder of the scale of the problems confronting all those involved. (shrink)

The heart has a high‐metabolic rate, and its “around‐the‐clock” vital role to sustain life sets it apart in a regenerative setting from other organs and appendages. The landscape of vertebrate species known to perform intrinsic heart regeneration is strongly biased toward ectotherms—for example, fish, salamanders, and embryonic/neonatal ectothermic mammals. It is hypothesized that intrinsic heart regeneration is exclusively limited to the low‐metabolic hearts of ectotherms. The biomedical field of regenerative medicine seeks to devise biologically inspired regenerative therapies to (...) diseased human hearts. Falsification of the ectothermy dependency for heart regeneration hypothesis may be a crucial prerequisite to meaningfully seek inspiration in established ectothermic regenerative animal models. Otherwise, engineering approaches to construct artificial heart components may constitute a more viable path toward regenerative therapies. A more strict definition of regenerative phenomena is generated and several testable sub‐hypotheses and experimental avenues are put forward to elucidate the link between heart regeneration and metabolism. Also see the video abstract here https://youtu.be/fZcanaOT5z8. (shrink)

Cells and tissue within injured organs undergo a complicated healing process that still remains poorly understood. Interestingly, smaller organisms respond to injury with tissue regeneration and restoration of function, while humans and other large organisms respond to injury by forming dysfunctional, fibrotic scar tissue. Over the past few decades, allometric scaling principles have been well established to show that larger organisms experience exponentially higher tissue forces during movement and locomotion and throughout the organism's lifespan. How these evolutionary adaptations may (...) affect tissue injury has not been thoroughly investigated in humans. We discuss how these adapations may affect healing and demonstrate that blocking the most evolutionary conserved biologic force sensor enables large organisms to heal after injury with true tissue regeneration. Future strategies to disrupt tissue force sensors may unlock the key to regenerating after injury in a wide range of organ systems. (shrink)

The great powers of regeneration shown by freshwater planarians, capable of regenerating a complete organism from any tiny body fragment, have attracted the interest of scientists throughout history. In 1814, Dalyell concluded that planarians could “almost be called immortal under the edge of the knife”. Equally impressive is the developmental plasticity of these platyhelminthes, including continuous growth and fission (asexual reproduction) in well‐fed organisms, and shrinkage (degrowth) during prolonged starvation. The source of their morphological plasticity and regenerative capability is (...) a stable population of totipotent stem cells—“neoblasts”; this is the only cell type in the adult that has mitotic activity and differentiates into all cell types. This cellular feature is unique to planarians in the Bilateria clade. Over the last fifteen years, molecular studies have begun to reveal the role of developmental genes in regeneration, although it would be premature to propose a molecular model for planarian regeneration. Genomic and proteomic data are essential in answering some of the fundamental questions concerning this remarkable morphological plasticity. Such information should also pave the way to understanding the genetic pathways associated with metazoan somatic stem‐cell regulation and pattern formation. BioEssays 28: 546–559, 2006. © 2006 Wiley Periodicals, Inc. (shrink)

Le projet révolutionnaire s'est largement identifié à un projet pédagogique, qui déborde de beaucoup les dispositifs scolaires pour s'attacher à une véritable conversion : du sujet au citoyen, de l'homme enchaîné à l'homme libre, du vieil homme à l'homme régénéré. Au coeur de cet ouvrage, on trouvera l'essai consacré à cette entreprise, dont Saint-Just a défini l'ambition ("faire des hommes ce qu'on veut qu'ils soient") et Mirabeau le possible délire : "Avec des moyens appropriés, on pourrait passionner les hommes pour (...) une organisation sociale entièrement absurde, injuste et cruelle". Toutes les études qui accompagnent ce texte central éclairent à leur manière la tentative utopique, magnifique et désespérée, de maîtriser à la fois l'événement et la durée, l'individu et le collectif, l'opinion réfléchie et l'opinion spontanée. Se dessine ainsi le vrai sujet de cet ensemble, que traitaient déjà La Fête révolutionnaire (1976) et L'École de la France (1984) : l'entrée, avec la volonté d'instituer l'homme aussi bien que le citoyen, dans notre culture démocratique. (shrink)

This collection of essays, drawn from the latest generation of Whitehead scholars, explores how, in the deconstruction of certain concepts, an unceasing invitation of possibility and change is released, both in relation to ongoing philosophical conversations, and as applied to lived experience. The essays make a significant intervention in the field of Whiteheadian scholarship by creating new intersections and paths that extend Whitehead's thought in novel, and often unexpected, directions. The philosophy of Alfred North Whitehead proposes a radical reconceptualization of (...) experience-one in which we, and all other things, are composed of mutually implicated series of events in an infinite universe of interaction, generating and regenerating experience. Far from indicating a new superlative of holistic integrity, Whitehead prefers the always incomplete movement of all realities, which is the source of vitality for every new generation. This volume applies Whitehead's philosophy to superlatives-those valued concepts that limit and define our categories amid the flux of experience. The first half of this book probes the superlatives that have historically defined philosophical method in the West. These essays trace the adventures of concepts like substance, novelty, system, and truth. Ossified oppositions that define these superlatives are fractured, indicating new directions for growth. The essays in the second half of the book reflect on the influx, fragility, and impossibility of superlatives like care, tragedy, love, and loss in human experience, generating new matters of philosophical discourse. Superlatives abound. But Whitehead cautions us to attend to their multiplicity. The mutual immanence of events constantly generates new constellations of importance, and so superlatives, because they are contingent upon unstable modes of togetherness, cannot be taken for granted. Any of these concepts may have a particular significance today, but as events coalesce into new constellations, those ideals will continue to take on new meaning. (shrink)

Axon regeneration is a conserved process across the animal kingdom. Recent studies using the soil worm Caenorhabditis elegans as a model system revealed that machineries regulating engulfment of dying cells also control axon regeneration and axon debris removal. In this review, the relationships between the engulfment machinery and the biological processes triggered by axon injury and subsequent axon regeneration drawn from divergent views are examined. In one study, it is found that engulfing cells directly promote axon (...) class='Hi'>regeneration. In this context, CED‐1 (Drosophila Draper/mouse MEGF10), an engulfment protein expressed on the surface of engulfing cells, functions as a receptor for axon debris removal and as an adhesion molecule for axon regeneration. In other studies, it is shown that those engulfment genes, previously known to function within the engulfing cells for cell corpse removal, can have a cell‐autonomous “non‐engulfing cell” role in axon regeneration. Together, these findings suggest that engulfment genes are repurposed for neuronal regeneration by acting in both engulfing cells and regenerating neurons. (shrink)

Ever since it was discovered in hydra, regeneration has remained a stimulating question for developmental biologists. Cellular approaches have revealed that, within the first few hours of apical or basal hydra regeneration, differentiation and determination of nerve cells are the primary cellular events detectable. The head and foot activators (HA, FA), neuropeptides that are released upon injury, are signaling molecules involved in these processes. In conditions where it induces cellular differentiation or determination, HA behaves as an agonist of (...) the cyclic AMP (cAMP) pathway involving the modulation of CREB nuclear transcription factor activity. This cascade would be required for proper regeneration, regardless of whether the polarity involved is apical or basal. Modulations of the protein kinase C pathway, which have been shown to affect apical or basal positional values, might signal to bring about this polarity; however, endogenous ligands responsible for this modulation are as yet unknown. (shrink)

In this article, the ability of partnerships to generate goods that enhance the quality-of-life of socially and economically deprived urban communities is explored. Drawing on Rawl's study on social justice [Rawls, J.: 1971, A Theory of Justice (Harvard University Press, Cambridge)] and Sen's capabilities approach [Sen, A.: 1992, Inequality Re-Examined (Harvard University Press, Cambridge, MA); 1999, Development as Freedom (Oxford University Press, Oxford); 2009, The Idea of Justice (Ellen Lane, London)], we undertake an ethical evaluation of the effectiveness of different (...) approaches to partnership activity in city neighbourhood regeneration. We focus, in particular, on their impact on the social regeneration of disadvantaged communities. Governance of cross-sector partnerships, built upon negotiated values and strong community voice, may result in a greater sense of procedural justice, as well as improvements to orderliness in local neighbourhoods. However, distributive justice, the accumulation of, and access to, goods that enable greater participation in society, remains largely elusive within neighbourhood partnership activity. We conclude that social provision that deals fairly with the causes of disadvantage by enhancing the capabilities of local communities and increasing social capital is likely to be a more effective and sustainable approach for partnerships, despite being a longer-term and more costly endeavour. (shrink)

In this study Professor Michalson attempts to clarify the complex tangle of issues connected with Kant's doctrines of radical evil and moral regeneration, and to set the problems resulting from these doctrines in an interpretive framework that tries to make sense of the instability of his overall position. In his late work Religion Within the Limits of Reason Alone, Kant charts out these doctrines in a manner that represents a fresh development in his own thinking on moral and relgious (...) matters, apparently at variance with the mainstream Enlightenment outlook which Kant otherwise embodies. His position appears to amount to a retrieval of the supposedly outmoded Christian doctrine of original sin, and this ambivalence is seen to stem from his desire to do justice both to the Protestant Christian, and the Enlightenment rationalist, tradition, which weigh equally heavily upon him. In this study Professor Michalson attempts to clarify the complex tangle of issues connected with Kant's doctrines of radical evil and moral regeneration, and to set the problems resulting from these doctrines in an interpretive framework that tries to make sense of the instability of his overall position. (shrink)

All cells have electric potentials across their membranes, but is there really compelling evidence to think that such potentials are used as instructional cues in developmental biology? Numerous reports indicate that, in fact, steady, weak bioelectric fields are observed throughout biology and function during diverse biological processes, including development. Bioelectric fields, generated upon amputation, are also likely to play a key role during vertebrate regeneration by providing the instructive cues needed to direct migrating cells to form a wound epithelium, (...) a structure unique to regenerating animals. However, mechanistic insight is still sorely lacking in the field. What are the genes required for bioelectric‐dependent cell migration during regeneration? The power of genetics combined with the use of zebrafish offers the best opportunity for unbiased identification of the molecular players in bioelectricity. BioEssays 29:1133–1137, 2007. © 2007 Wiley Periodicals, Inc. (shrink)