We show that if R is a nonconstant regular (semi-)local subring of a rational function field over an algebraically closed field of characteristic zero, Hilbert's Tenth Problem for this ring R has a negative answer; that is, there is no algorithm to decide whether an arbitrary Diophantine equation over R has solutions over R or not. This result can be seen as evidence for the fact that the corresponding problem for the full rational field is also unsolvable.