Investigating Metalloproteinases MMP-2 and MMP-9 Mechanosensitivity to Feedback Loops Involved in the Regulation of In Vitro Angiogenesis by Endogenous Mechanical Stresses [Book Review]
Acta Biotheoretica 60 (1-2):21-40 (2012)
Angiogenesis is a complex morphogenetic process regulated by growth factors, but also by the force balance between endothelial cells traction stresses and extracellular matrix viscoelastic resistance. Studies conducted with in vitro angiogenesis assays demonstrated that decreasing ECM stiffness triggers an angiogenic switch that promotes organization of EC into tubular cords or pseudo-capillaries. Thus, mechano-sensitivity of EC with regard to proteases secretion, and notably matrix metalloproteinases , should likely play a pivotal role in this switching mechanism. While most studies analysing strain regulation of MMPs used cell cultured on stretched membranes, this work focuses on MMP expression during self-assembly of EC into capillary-like structures within fibrin gels, i.e. on conditions that mimics more closely the in vivo cellular mechanical microenvironment. The activity of MMP-2 and MMP-9, two MMPs that have a pivotal role in capillaries formation, has been monitored in pace with the progressive elongation of EAhy926 cells that takes place during the emergence of cellular cords. We found an increase of the zymogen proMMP-2 that correlates with the initial stages of EC cords formation. However, MMP-2 was not detected. ProMMP-9 secretion decreased, with levels of MMP-9 kept at a rather low value. In order to analyse more precisely the observed differences of EAhy926 response on fibrin and plastic substrates, we proposed a theoretical model of the mechano-regulation of proMMP-2 activation in the presence of type 2 tissue inhibitor of MMPs . Using association/dissociation rates experimentally reported for this enzymatic network, the model adequately describes the synergism of proMMP-2 and TIMP-2 strain activation during pseudo-capillary morphogenesis. All together, these results provide a first step toward a systems biology approach of angiogenesis mechano-regulation by cell-generated extracellular stresses and strains
|Keywords||No keywords specified (fix it)|
|Categories||categorize this paper)|
References found in this work BETA
No references found.
Citations of this work BETA
No citations found.
Similar books and articles
Predictive Power of “A Minima” Models in Biology.L. Almeida & J. Demongeot - 2012 - Acta Biotheoretica 60 (1-2):3-19.
The Role of Calcium in the Recall of Stored Morphogenetic Information by Plants.Marie-Claire Verdus, Camille Ripoll, Vic Norris & Michel Thellier - 2012 - Acta Biotheoretica 60 (1-2):83-97.
Reduced Models for Unidirectional Block Conduction and Their Geometrical Setting.L. El Alaoui, J. Francoise & M. Landau - 2012 - Acta Biotheoretica 60 (1-2):131-137.
Influence of Sparkle and Saccades on Tongue Electro-Stimulation-Based Vision Substitution of 2D Vectors.Abdessalem Chekhchoukh & Nicolas Glade - 2012 - Acta Biotheoretica 60 (1-2):41-53.
A Mechanical Model for the Formation of Vascular Networks in Vitro.D. Manoussaki, S. R. Lubkin, R. B. Vemon & J. D. Murray - 1996 - Acta Biotheoretica 44 (3-4):271-282.
Mathematical Modeling of Metabolism and Hemodynamics.J. Francoise, C. Menuel, M. Lahutte & J. -N. Vallée - 2012 - Acta Biotheoretica 60 (1-2):99-107.
Transmission of Mechanical Stresses Within the Cytoskeleton of Adherent Cells: A Theoretical Analysis Based on a Multi-Component Cell Model.Philippe Tracqui & Jacques Ohayon - 2004 - Acta Biotheoretica 52 (4):323-341.
On the Nature and Shape of Tubulin Trails: Implications on Microtubule Self-Organization.Nicolas Glade - 2012 - Acta Biotheoretica 60 (1-2):55-82.
Modelling Biological Gel Contraction by Cells: Mechanocellular Formulation and Cell Traction Force Quantification.I. Ferrenq, L. Tranqui, B. Vailhé, P. Y. Gumery & P. Tracqui - 1997 - Acta Biotheoretica 45 (3-4):267-293.
Parthenius (A.) Zucker (Ed.) Littérature Et Érotisme Dans les Passions d'Amour de Parthénios de Nicée. Actes du Colloque de Nice 31 Mai 2006. Pp. 218. Grenoble: Éditions Jérôme Millon, 2008. Paper, €25. ISBN: 978-2-84137-218-8. (M.) Biraud, (D.) Voisin, (A.) Zucker (Edd., Trans.) with (E.) Delbey, (K.) Vanhaegendoren, (F.) Wendling, (B.) Charlet Parthénios de Nicée: Passions d'Amour. Pp. 314. Grenoble: Éditions Jérôme Millon, 2008. Paper, €26. ISBN: 978-2-84137-217-. [REVIEW]Alexander Sens - 2010 - The Classical Review 60 (01):63-.
A Proliferation Control Network Model: The Simulation of Two-Dimensional Epithelial Homeostasis.Didier Morel, Raphaël Marcelpoil & Gérard Brugal - 2001 - Acta Biotheoretica 49 (4):219-234.
B. Colombat: La Grammaire Latine En France À la Renaissance Et À l' Âge Classique. Théories Et Pédagogie . Pp. 724. Grenoble: Ellug Université Stendhal, 1999. Paper, Frs. 390. ISBN: 2-84310-015-. [REVIEW]Ingrid de Smet - 2001 - The Classical Review 51 (02):456-.
The Mathematical Modelling of Tumour Angiogenesis and Invasion.M. A. J. Chaplain - 1995 - Acta Biotheoretica 43 (4):387-402.
Attitude, Affectivity and Prediction.Arthur Bultmann Grenoble - 1975 - Journal of Value Inquiry 9 (4):312-314.
Pattern Formation in a Nonlinear Membrane Model for Epithelial Morphogenesis.Larry A. Taber - 2000 - Acta Biotheoretica 48 (1):47-63.
Added to index2012-01-28
Total downloads7 ( #528,924 of 2,178,175 )
Recent downloads (6 months)2 ( #166,021 of 2,178,175 )
How can I increase my downloads?