'Characterization of the human beta-secretase 2 5'-flanking region: identification of a 268-bp region as the basal BACE2 promoter [Book Review]

J Mol Neurosci 29:81-99 (2006)
  Copy   BIBTEX

Abstract

The main characteristic of Alzheimer's disease is brain deposition of the beta-amyloid peptide, generated endoproteolytically from Abeta precursor protein by beta- and gamma-secretases. A transmembrane aspartyl protease, beta-APP-cleaving enzyme , was identified as beta-secretase. Although BACE1 cleaves APP at the beta-secretase site, the role of its homolog, beta-secretase 2 is poorly understood. We report the mRNA expression profile, DNA sequence, and molecular characterization of the BACE2 gene, located on chromosome 21q22.3. The BACE2 gene expresses more strongly in peripheral tissues, although BACE2 mRNA is found in the majority of brain regions, including the postcentral gyrus and temporal lobe. Characterization of 2932 bp of the BACE2 5'-flanking region , reveals the absence of canonical CCAAT and TATA boxes within 1 kb of the transcription start site . The sequence lacks significant internal repeats and has a housekeeping gene structure. Two active regions of the BACE2 promoter determine its basal expression and cell-type specificity. The proximal region likely determines general basal expression, and the distal region , cell-type specificity. Several putative transcription factor sites, particularly SP1, Oct-1, and HES-1, are predicted to be within 1 kb of the TSS. On either side of the proximal promoter region, two negative regulatory domains might reduce BACE2 expression under an induced condition. The BACE2 5'-flanking region is likely to be highly regulated and expressed in a tissue type-specific manner

Links

PhilArchive



    Upload a copy of this work     Papers currently archived: 94,385

External links

Setup an account with your affiliations in order to access resources via your University's proxy server

Through your library

Similar books and articles

Analytics

Added to PP
2015-06-29

Downloads
4 (#1,658,004)

6 months
4 (#1,037,980)

Historical graph of downloads
How can I increase my downloads?

Citations of this work

No citations found.

Add more citations

References found in this work

No references found.

Add more references