Gene 488:1-12 (2011)

Authors
Bryan Maloney
Indiana University Purdue University, Indianapolis
Abstract
Deposition of extracellular plaques, primarily consisting of amyloid beta peptide , in the brain is the confirmatory diagnostic of Alzheimer's disease ; however, the physiological and pathological role of Abeta is not fully understood. Herein, we demonstrate novel Abeta activity as a putative transcription factor upon AD-associated genes. We used oligomers from 5'-flanking regions of the apolipoprotein E , Abeta-precursor protein and beta-amyloid site cleaving enzyme-1 genes for electrophoretic mobility shift assay with different fragments of the Abeta peptide. Our results suggest that Abeta bound to an Abeta-interacting domain with a consensus of "KGGRKTGGGG". This peptide-DNA interaction was sequence specific, and mutation of the first "G" of the decamer's terminal "GGGG" eliminated peptide-DNA interaction. Furthermore, the cytotoxic Abeta25-35 fragment had greatest DNA affinity. Such specificity of binding suggests that the AbetaID is worth of further investigation as a site wherein the Abeta peptide may act as a transcription factor
Keywords No keywords specified (fix it)
Categories No categories specified
(categorize this paper)
DOI 10.1016/j.gene.2011.06.004
Options
Edit this record
Mark as duplicate
Export citation
Find it on Scholar
Request removal from index
Revision history

Download options

Our Archive


Upload a copy of this paper     Check publisher's policy     Papers currently archived: 50,308
External links

Setup an account with your affiliations in order to access resources via your University's proxy server
Configure custom proxy (use this if your affiliation does not provide a proxy)
Through your library

References found in this work BETA

No references found.

Add more references

Citations of this work BETA

Add more citations

Similar books and articles

Analytics

Added to PP index
2015-06-29

Total views
6 ( #1,025,180 of 2,325,997 )

Recent downloads (6 months)
1 ( #658,522 of 2,325,997 )

How can I increase my downloads?

Downloads

My notes