A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection

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Abstract

Posttranslational modification of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.

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Author Profiles

Tingxue Wei
Xiamen University
Chen Liu
University of Edinburgh
Xincheng Li
Nankai University
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