An integrated robotic laser and microscope system has been developed to automatically analyze individual sperm motility and energetics. The custom-designed optical system directs near-infrared laser light into an inverted microscope to create a single-point 3-D gradient laser trap at the focal spot of the microscope objective. A two-level computer structure is described that quantifies the sperm motility and energetics using real-time tracking and fluorescent ratio imaging. The communication between these two systems is achieved by a gigabit network. The custom-built image processing algorithm identifies the sperm swimming trajectory in real-time using phase contrast images, and then subsequently traps the sperm by automatically moving the microscope stage to relocate the sperm to the laser trap focal plane. Once the sperm is stably trapped, the algorithm can also gradually reduce the laser power by rotating the polarizer in the laser path to measure the trapping power at which the sperm is capable of escaping the trap. To monitor the membrane potential of the mitochondria located in a sperm's mid-piece, the sperm is treated with a ratiometrically-encoded fluorescent probe. The proposed algorithm can relocate the sperm to the center of the ratio imaging camera and the average ratio value can be measured in real-time. The three parameters, sperm escape power, sperm swimming speed and ratio values of the mid-piece membrane potential of individual sperm can be compared with respect to time. This two-level automatic system to study individual sperm motility and energetics has not only increased experimental throughput by an order of magnitude but also has allowed us to monitor sperm energetics prior to and after exposure to the laser trap. This system should have application in both the human fertility clinic and in animal husbandry. © Springer Science+Business Media, LLC 2008.
Keywords No keywords specified (fix it)
Categories No categories specified
(categorize this paper)
Edit this record
Mark as duplicate
Export citation
Find it on Scholar
Request removal from index
Revision history

Download options

PhilArchive copy

Upload a copy of this paper     Check publisher's policy     Papers currently archived: 63,319
External links

Setup an account with your affiliations in order to access resources via your University's proxy server
Configure custom proxy (use this if your affiliation does not provide a proxy)
Through your library

References found in this work BETA

No references found.

Add more references

Citations of this work BETA

No citations found.

Add more citations

Similar books and articles

Woman Wants Dead Fiance's Baby: Who Owns a Dead Man's Sperm.M. Spriggs - 2004 - Journal of Medical Ethics 30 (4):384-388.
The Moral Complexity of Sperm Donation.Rivka Weinberg - 2008 - Bioethics 22 (3):166–178.
Sperm Donor Suffers Years Later From Inherited Disease.D. O. E. Gebhardt - 2002 - Journal of Medical Ethics 28 (4):213-214.


Added to PP index

Total views
3 ( #1,322,004 of 2,448,695 )

Recent downloads (6 months)
3 ( #224,503 of 2,448,695 )

How can I increase my downloads?


Sorry, there are not enough data points to plot this chart.

My notes