We have synthesized a 582,970-base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted "watermarks" at intergenic sites known to tolerate transposon insertions. Overlapping "cassettes" of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate (...) assemblies of approximately 24 kb, 72 kb ("1/8 genome"), and 144 kb ("1/4 genome"), which were all cloned as bacterial artificial chromosomes in Escherichia coli. Most of these intermediate clones were sequenced, and clones of all four 1/4 genomes with the correct sequence were identified. The complete synthetic genome was assembled by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae, then isolated and sequenced. A clone with the correct sequence was identified. The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments. 10.1126/science.1151721. (shrink)
Graphical displays of simple moving geometrical figures have been repeatedly used to study the attribution of animacy in human observers. Yet little is known about the relevant movement characteristics responsible for this experience. The present study introduces a novel parametric research paradigm, which allows for the experimental control of specific motion parameters and a predictable influence on the attribution of animacy. Two experiments were conducted using 3D computer animations of one or two objects systematically introducing variations in the following aspects (...) of motion: directionality, discontinuity and responsiveness. Both experiments further varied temporal kinematics. Results showed that animacy experience increased with the time a moving object paused in the vicinity of a second object and with increasing complexity of interaction between the objects . The experience of animacy could be successfully modulated in a parametric fashion by the systematic variation of comparably simple differential movement characteristics. (shrink)
British cultural studies, represented perhaps chiefly by the so-called Birmingham School, is much marked by its strong orientation towards the application of grounded theory in the analysis of concrete cases, rather than the development of abstract Theory with a Capital T . As a leading figure of the Birmingham School and a key representative of the active audience model in television studies, or broadly, media studies, DavidMorley stands at a point where this trend was set, as is (...) evidenced in this interview. Questioned by Huimin Jin, Morley puts his audience studies into the contexts of British cultural studies, postmodernism, Marxism, social movements, and so on; and in doing so, he shows the ambiguity, and subtlety of his concepts of how to best theorize the active audience. Only by this method, Jin believes, could Morley launch his version of audience studies, which aims not to invent a general theory of media effects, but to use an interdisciplinary range of theories to explore how people actually respond to a TV programme; and only by this approach to audience studies, furthermore, could Morley develop a theory of the audience’s activity, which is embedded in the course of their everyday life and that cannot be thoroughly colonized by discourses. Cultural studies, wherever it is conducted, therefore, Morley suggests, has to construct modes of analysis that are relevant to its own conditions of production in a particular place, at a particular time. This is the tradition, as we know it, but also the future, as Morley envisages, of cultural studies. (shrink)
Reviews : Zygmunt Bauman, Intimations of Postmodernity ; Steven Seidman and David G. Wagner , Postmodernism and Social Theory ; Stephen Crook, Jan Pakulski and Malcolm Wa ters, Postmodernization: Change in Advanced Society ; Gianni Vattimo, The End of Modernity—Nihilism and Hermeneutics in Post-modern Culture.
Wittgenstein's Method: Neglected Aspects By Gordon Baker. Oxford: Blackwell, 2004 pp. 328. £40.00 HB.. Wittgenstein's Copernican Revolution: The Question of Linguistic Idealism By Ilham Dilman. Basingstoke: Palgrave, 2002. pp. 240. £52.50 HB. Wittgenstein: Connections and Controversies By P. M. S. Hacker. Oxford: Oxford University Press,. pp. 400. £45.00 HB; £19.99 PB. Wittgenstein's Philosophical Investigations: An Introduction By David G. Stern. Cambridge: Cambridge University Press, 2004. pp. 224. £40.00 HB; £10.99 PB.
Elias G. Carayannis and David F. J. Campbell, Mode 3 Knowledge Production in Quadruple Helix Innovation Systems: 21st-Century Democracy, Innovation, and Entrepreneurship for Development Content Type Journal Article Category Book Review Pages 139-142 DOI 10.1007/s11024-012-9194-6 Authors Barbara Prainsack, Department of Sociology and Communications, Brunel University, Kingston Lane, Uxbridge, Middlesex UB8 3PH, UK Journal Minerva Online ISSN 1573-1871 Print ISSN 0026-4695 Journal Volume Volume 50 Journal Issue Volume 50, Number 1.