We have synthesized a 582,970-base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted "watermarks" at intergenic sites known to tolerate transposon insertions. Overlapping "cassettes" of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate (...) assemblies of approximately 24 kb, 72 kb ("1/8 genome"), and 144 kb ("1/4 genome"), which were all cloned as bacterial artificial chromosomes in Escherichia coli. Most of these intermediate clones were sequenced, and clones of all four 1/4 genomes with the correct sequence were identified. The complete synthetic genome was assembled by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae, then isolated and sequenced. A clone with the correct sequence was identified. The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments. 10.1126/science.1151721. (shrink)
In face of the multiple controversies surrounding the DSM process in general and the development of DSM-5 in particular, we have organized a discussion around what we consider six essential questions in further work on the DSM. The six questions involve: 1) the nature of a mental disorder; 2) the definition of mental disorder; 3) the issue of whether, in the current state of psychiatric science, DSM-5 should assume a cautious, conservative posture or an assertive, transformative posture; 4) the role (...) of pragmatic considerations in the construction of DSM-5; 5) the issue of utility of the DSM - whether DSM-III and IV have been designed more for clinicians or researchers, and how this conflict should be dealt with in the new manual; and 6) the possibility and advisability, given all the problems with DSM-III and IV, of designing a different diagnostic system. Part 1 of this article took up the first two questions. Part 2 took up the second two questions. Part 3 now deals with Questions 5 & 6. Question 5 confronts the issue of utility, whether the manual design of DSM-III and IV favors clinicians or researchers, and what that means for DSM-5. Our final question, Question 6, takes up a concluding issue, whether the acknowledged problems with the earlier DSMs warrants a significant overhaul of DSM-5 and future manuals. As in Parts 1 & 2 of this article, the general introduction, as well as the introductions and conclusions for the specific questions, are written by James Phillips, and the responses to commentaries are written by Allen Frances. (shrink)
This book is a collection of secondary essays on America's most important philosophic thinkers—statesmen, judges, writers, educators, and activists—from the colonial period to the present. Each essay is a comprehensive introduction to the thought of a noted American on the fundamental meaning of the American regime.
The contribution focuses on philosophical issues of justice of positive law in the light of the social teaching of John Paul II. The analyses start with consideration of anthropological foundations of justice as virtue, develop with the reflexion upon justice of actions realizing justice and finally arrive at examination of the criteria of justice of law. -/- It is argued that relations between a human being and goods (ends of actions) form ontological basis of natural law and justice of (...) actions – orders and prohibitions are secondary in respect to these relations. An aim of just law (and natural law) is not preservation or restoration of abstractly understood moral order based on norms – orders and prohibitions) but integral development (good) of a person – a being possessing dignity. John Paul’s II philosophy of law takes advantage primarily of Thomas Aquinas’ approach to law and combines it with constructions which are typical for modern human rights protection. John Paul’s II conception of natural law is anthropocentric and bases on subjective rights thinking. Human dignity and human rights which derive from it provide basic criteria for the justice of law. Human rights as subjective rights disclose natural law which is understood as a set of goods for a human person. These goods are ends of actions and as such they determine actions and their forms. This point of view is compatible with Aquinas’ definition: “law is nothing but a rational plan of operation, and … the rational plan of any kind of work is derived from the end” (Summa contra gentiles, lib. 3, cap. 114, n. 5). -/- Positive (human) law which is not just has no normative power in this sense that it does not in itself provide reasons for concrete actions of a concrete actor. Sometimes there are moral reasons for following unjust law, however if its norm prescribes actions which are wrong in themselves (internally wrong) there is moral obligation to act contrary to such a legal norm. -/- Zasadniczym przedmiotem opracowania jest filozoficzna refleksja Jana Pawła II nad sprawiedliwością prawa stanowionego. Analizy przebiegają od zagadnienia antropologicznych podstaw sprawiedliwości poprzez problematykę działań realizujących sprawiedliwość do zagadnienia sprawiedliwości prawa stanowionego. Opracowanie zamykają uwagi wskazujące na kontekst teologiczny istotny dla problematyki sprawiedliwości, którego analiza wykracza jednak poza podjęte zamierzenie koncentrujące się na problematyce filozoficznoprawnej. Argumentuje się, że u podstaw tej refleksji leży namysł nad relacją człowieka do dobra, która stanowi ontologiczną podstawę prawa naturalnego i sprawiedliwości – nakazy i zakazy są wtórne wobec tej relacji. Celem prawa i sprawiedliwości jest dobro konkretnego, obdarzonego godnością człowieka, a nie np. przywracanie abstrakcyjnie pojętego porządku moralnego. Od strony konstrukcji teoretycznej, filozofia prawa Jana Pawła II jest osadzona przede wszystkim na koncepcji Tomasza z Akwinu łączonej z konstrukcjami typowymi dla współczesnej ochrony praw człowieka. To w godności i wynikających z niej prawach człowieka poszukiwać trzeba zasadniczych treściowych kryteriów sprawiedliwości prawa. Prawa człowieka jako prawa podmiotowe są podstawowym wyrazem prawa naturalnego, stanowiącego ontyczną podstawę sprawiedliwości i które pojmowane jest jako zespół dóbr dla osoby, zatem i celów kształtujących działanie. Perspektywa pojmowania prawa naturalnego jest antropocentryczna. Prawo stanowione, które nie jest sprawiedliwe, nie ma „mocy prawa”, przede wszystkim w takim sensie, że nie stanowi samo w sobie racji działania. Niekiedy, ze względów moralnych, niesprawiedliwe prawo wymaga posłuszeństwa. Jeśli jednak prawo stanowione daje uprawnienia do czynów wewnętrznie złych i nakazuje takie czyny, to nie tylko nie obowiązuje w sumieniu i nie jest racją działania, ale obowiązkiem jest postępowanie wbrew takiemu prawu. (shrink)
In this ambitious study, Alexander W. Hall examines the two preeminent figures of the golden age of natural theology: Thomas Aquinas and John Duns Scotus. Hall is not so much concerned with retracing particular proofs of the existence of God and derivations of the divine attributes—well-worn paths in discussions of medieval natural theology—as with investigating the larger philosophical issues that are raised by the project of natural theology, such as the nature of scientia and demonstrative arguments, and accounts (...) of signification and the meaningfulness of theological discourse.Hall's opening chapter offers an overview of natural theology in the High Middle Ages, summarizing the conclusions he will defend at greater length over the course of the book. In chapter 2 Hall relies primarily on Aquinas's commentary on the Posterior Analytics to get clear on his account of scientia, or scientific knowledge. "For Aquinas," Hall writes,"paradigmatic scientia is the result of syllogistic reasoning . . . Syllogisms productive of scientia use either real or nominal definitions as their middle, and thus the conclusion tells us what belongs to the subject through itself or per se". (shrink)
Celia Wolf‐Devine: Descartes on Seeing: Epistemology and Visual Perception. Carbondale and Edwardsville: Southern Illinois University Press, 1993, pp. viii + 121. ISBN 0–8093–1838–5. Thomas Hobbes: Leviathan with selected variants front the Latin edition of 1668. Edited, with Introduction and Notes by Edwin Curley. Hackett Publishing Company, Inc., Indianapolis/cambridge 1994, pp. lxxx‐584. ISBN 0–87220–178–3, £27.95, 0–87220–177–5, £6.95. Allison Coudert: Leibniz and the Kabbalah. Dordrecht: Kluwer Academic Publishers, 1995, pp. 218. £68.00. ISBN 0–7923–3114–1. Richard Price: The Correspondence. [Edited by D. O. (...)Thomas and W. Bernard Peach]. Vol. III. February 1786‐February 1791. Edited by W. Bernard Peach.. ISBN 0–8223–1327–8. Henry Allison: Idealism and Freedom: Essays on Kant's Theoretical and Practical Philosophy. Cambridge University Press, 1996. xxi + 217 pp. £30, £10.95. ISBN 0–521–48295‐X, 0–521–48337–9. Terry Pinkard: Hegel's Phenomenology: The Sociality of Reason. Cambridge University Press, 1994. 4451 pp. £40.00 hb. ISBN 0–521–45300–3. Mary Anne Perkins: Coleridge's Philosophy, The Logos as Unifying Principle. pp. 310. £30.00. ISBN 0–19–824075–9. Elzbieta Ettinger: Hannah Arendt ‐ Martin Heidegger £10.95 ISBN 0–300–06407–1 Dana R. Villa: Arendt and Heidegger ‐ The Fate of the Political ISBN 0–691–04400–7. (shrink)
In 1277, Stephen Tempier, bishop of Paris, drafted the famous Condemnation of 219 articles in theology and natural philosophy. This Condemnation was a reaction against a group of theologians, led by Siger of Brabant, who were accused of holding that truths of reason could contradict those of revelation. Writing before the Condemnation, which impugned reason's autonomy, Thomas Aquinas critiqued Siger and his followers, and argued that reason could never generate truths that contradict revelation. As a consequence, Aquinas sometimes dwells (...) on reason's limits, terming our knowledge of God 'equivocal' or 'analogical'. Sitting on the other side of the Condemnation, around the turn of the fourteenth century, John Duns Scotus was concerned to secure for reason a portion of its lost dignity. Accordingly, he explores what we can know of God, and lays claim to 'univocal' knowledge of him. Some scholars hold that Scotus's claim to univocal knowledge of God puts him at odds with Aquinas. In this dissertation, I argue that this is not the case, that this apparent discord is largely the result of their different enterprises rather than their basic beliefs about our natural knowledge of God, that Scotus's discussion of univocity is targeted not at Aquinas, but rather at Henry of Ghent---the leading light of the University of Paris at the time that Scotus studied there---and that Scotus does not believe that our knowledge of God is univocal in a strong sense, such that he would claim that it is wholly accurate. Rather, Scotus believes that, in this life, our knowledge of God must comprise abstract concepts that can never do justice to their source. This being the case, he has more in common with Aquinas than some would allow. (shrink)
It has been shown that the thirteenth-century Dominican friar, St Thomas Aquinas, was an important theological influence on John Owen, the seventeenth-century English puritan theologian, chaplain to Oliver Cromwell, and Vice-Chancellor of Oxford University, especially in the areas of the divine being, grace and Chalcedonian Christology. Suzanne McDonald has argued that, while Aquinas is unmistakably a source for Owen's doctrine of the beatific vision, Owen surpassed Aquinas's doctrine in a manner she judges to be correct, theologically speaking, and (...) which exposes the deficiency of Aquinas's account. Owen achieved this particular ‘Reforming’ or rather ‘re-forming’ of Aquinas's doctrine, she argues, by way of a ‘Christological re-orientation of the doctrine’ in terms of what is seen in the beatific vision and how it is seen, that is, its content and means. This article replies to McDonald from a Catholic and Thomist perspective, in response to her suggestion that Owen's account of the beatific vision opens up possibilities for ecumenical dialogue. The article attempts to achieve this first by reassessing the Christological contrasts McDonald draws between Owen and Aquinas in terms of content and means, and then by offering several suggestions as to why one might want to prefer Aquinas's account over Owen's. (shrink)
Thomas M. Osborne Jr. ... Vivarium 32 (1994): 62–71. te Velde, Rude A. “Natura in se ipsa recurva est: Duns Scotus and Aquinas on the Relationship between Nature and Will.” In John Duns Scotus: ... “William of Ockham's Theological Ethics .
There are two general routes that Augustine suggests in De Trinitate, XV, 14-16, 23-25, for a psychological account of the Father's intellectual generation of the Word. Thomas Aquinas and Henry of Ghent, in their own ways, follow the first route; John Duns Scotus follows the second. Aquinas, Henry, and Scotus's psychological accounts entail different theological opinions. For example, Aquinas (but neither Henry nor Scotus) thinks that the Father needs the Word to know the divine essence. If we compare (...) the theological views entailed by their psychologies we find a trajectory from Aquinas, through Henry, and ending with Scotus. This theological trajectory falsifies a judgment that every Augustinian psychology of the divine persons amounts to a pre-Nicene functional Trinitarianism. This study makes clear how one's awareness of the theological views entailed by these psychologies enables one to assess more thoroughly psychological accounts of the identity and distinction of the divine persons. (shrink)