Throughout the biological and biomedical sciences there is a growing need for, prescriptive ‘minimum information’ (MI) checklists specifying the key information to include when reporting experimental results are beginning to find favor with experimentalists, analysts, publishers and funders alike. Such checklists aim to ensure that methods, data, analyses and results are described to a level sufficient to support the unambiguous interpretation, sophisticated search, reanalysis and experimental corroboration and reuse of data sets, facilitating the extraction of maximum value from data sets (...) them. However, such ‘minimum information’ MI checklists are usually developed independently by groups working within representatives of particular biologically- or technologically-delineated domains. Consequently, an overview of the full range of checklists can be difficult to establish without intensive searching, and even tracking thetheir individual evolution of single checklists may be a non-trivial exercise. Checklists are also inevitably partially redundant when measured one against another, and where they overlap is far from straightforward. Furthermore, conflicts in scope and arbitrary decisions on wording and sub-structuring make integration difficult. This presents inhibit their use in combination. Overall, these issues present significant difficulties for the users of checklists, especially those in areas such as systems biology, who routinely combine information from multiple biological domains and technology platforms. To address all of the above, we present MIBBI (Minimum Information for Biological and Biomedical Investigations); a web-based communal resource for such checklists, designed to act as a ‘one-stop shop’ for those exploring the range of extant checklist projects, and to foster collaborative, integrative development and ultimately promote gradual integration of checklists. (shrink)

Variations in levels of apolipoprotein E have been tied to the risk and progression of Alzheimer's disease . Our group has previously compared and contrasted the promoters of the mouse and human ApoE gene promoter sequences and found notable similarities and significant differences that suggest the importance of the APOE promoter's role in the human disease. We examine here three specific single-nucleotide polymorphisms within the human APOE promoter region, specifically at -491 , -427 , and at -219 upstream from the (...) +1 transcription start site. The -219 and -491 polymorphic variations have significant association with instance of AD, and -491AA has significant risk even when stratified for the APOEepsilon4 allele. We also show significant effects on reporter gene expression in neuronal cell cultures, and, notably, these effects are modified by species origin of the cells. The -491 and -219 polymorphisms may have an interactive effect in addition to any independent activity. DNA-protein interactions differ between each polymorphic state. We propose SP1 and GATA as candidates for regulatory control of the -491 and -219 polymorphic sites. This work's significance lies in drawing connection among APOE promoter polymorphisms' associations with AD to functional promoter activity differences and specific changes in DNA-protein interactions in cell culture-based assays. Taken together, these results suggest that APOE expression levels are a risk factor for AD irrespective of APOEepsilon4 allele status. (shrink)

For 60 years, Herbert Schneider has been making notable contributions to philosophy. In 1972, at a surprise party for his 80th birthday, friends presented him with a collection of essays on areas of philosophy in which he himself had done pioneering work. These essays, together with five previously published but difficult-to-find papers written by Schneider himself, are included in the present book, along with a biographical sketch of Schneider prepared by the editors and a list of Schneider’s writings. Among the (...) better-known contributors are Joseph L. Blau, Max Fisch, Lewis Hahn, George Kline, Paul Kurtz, and Richard H. Popkin. The essays include historical studies in ancient and modern philosophy as well as analytical studies in social theory and problems of education. (shrink)

For 60 years, Herbert Schneider has been making notable contributions to philosophy. In 1972, at a surprise party for his 80th birthday, friends presented him with a collection of essays on areas of philosophy in which he himself had done pioneering work. These essays, together with five previously published but difficult-to-find papers written by Schneider himself, are included in the present book, along with a biographical sketch of Schneider prepared by the editors and a list of Schneider’s writings. Among the (...) better-known contributors are Joseph L. Blau, Max Fisch, Lewis Hahn, George Kline, Paul Kurtz, and Richard H. Popkin. The essays include historical studies in ancient and modern philosophy as well as analytical studies in social theory and problems of education. (shrink)