Since most of the functions in cells are mediated by multimeric protein complexes, the determination of protein–protein interactions is an important step in the study of cellular mechanisms. Traditionally, after screening for possible target interactors by means of a yeast two‐hybrid screen, several methods are used to validate the initial result before carrying out functional experiments. Nowadays, non‐invasive fluorescence‐based methods like Bioluminescence Resonance Energy Transfer (BRET) and Fluorescence Resonance Energy Transfer (FRET) are widely used in the study of protein–protein interactions (...) in living cells. In the present review, we address the individual strengths and weaknesses of both RET approaches, providing information on their possible future use in the study of G protein‐coupled receptor oligomerization. BioEssays 30:82–89, 2008. © 2007 Wiley Periodicals, Inc. (shrink)

In June 2021, new waves of the current COVID-19 pandemic are still messing up our lives and magnetizing the compass of our life. No other epidemics or pandemics have been politicized at such a level since ancient times. The full political spectrum from right to left has tried to ride this pandemic and upset the public health response efforts. Statistics and politics have changed our daily approach to this brutal infection, but even crueler has been the palpable miscommunication. Massive and (...) uncontrolled lockdowns and not climate change are destroying the economy of our planet. Intolerance against vaccine opponents and hesitant families has been vocalized with martial tones in both social media and news channels. In this book, the author analyzes the pandemic in a historical context, emphasizes the importance of facemasks, but also highlights our human approach to anti-mask psychology. It would be appalling to call mistakes in public health decisions "learned lessons" because it reduces the gravity of the consequences of inadequate communication. Our youth is our future. The mental issues arising from a lack of social interaction and in-person education are dreadful. Vaccines are powerful and represent the most outstanding progress in medicine. There is light at the end of the tunnel, but vaccine acceptance or herd immunity is not the answer, or probably, not the only one. There is true hope that we are going back to our world, but the COVID-19 pandemic may be an occasion to reconsider the traditional family in the center of our lives and daily politics. A tolerance towards any race needs to be contagious, militant, and blazing to vehemently rebuild and revitalize the American Dream. (shrink)

For centuries, the Sorites Paradox has spurred philosophers to think and argue about the problem of vagueness. This volume offers a guide to the paradox which is both an accessible survey and an exposition of the state of the art, with a chapter-by-chapter presentation of all of the main solutions to the paradox and of all its main areas of influence. Each chapter offers a gentle introduction to its topic, gradually building up to a final discussion of some open problems. (...) Students will find a comprehensive guide to the fundamentals of the paradox, together with lucid explanations of the challenges it continues to raise. Researchers will find exciting new ideas and debates on the paradox. (shrink)

This edited collection presents the state of the art in research related to lexical combinations and their restrictions in Spanish from a variety of theoretical approaches, ranging from Explanatory Combinatorial Lexicology to Distributed Morphology and Generative Lexicon Theory. Section 1 offers a presentation of the main theoretical and descriptive approaches to collocation. Section 2 explores collocation from the point of view of its lexicographical representation, while Section 3 offers a pedagogical perspective. Section 4 surveys current research on collocation in Catalan, (...) Galician and Basque. Collocations and other lexical combinations in Spanish will be of interest to students of Hispanic linguistics. ". (shrink)

Lloyd (2011) presents highly suggestive results regarding the specificity of the link between particular brain areas and cognitive tasks. Some of his evidence is derived from the analysis of data from the BrainMap database (available: www.brainmap.org), which has become a fundamental resource for the conduct of functional neuroimaging meta-analysis. In the present note, some observations regarding the possibilities and pitfalls of meta-analysis of functional neuroimaging data are given as a complement to Lloyd's excellent exposition of the topic. Additionally, some comments (...) are made on the particular meta-analytic results presented by Lloyd.Functional neuroimaging studies usually present their findings in the form .. (shrink)

Research suggests that the ability to understand one’s own and others’ minds, or mentalizing, is a key factor for mental health. Most studies have focused the attention on the association between global measures of mentalizing and specific disorders. In contrast, very few studies have analyzed the association between specific mentalizing polarities and global measures of mental health. This study aimed to evaluate whether self and other polarities of mentalizing are associated with a multidimensional notion of mental health, which considers symptoms, (...) functioning, and well-being. Additionally, the level or depth of mentalizing within each polarity was also analyzed. A sample of 214 adolescents was evaluated on measures of self- and other- mentalizing, multi-informed measures of psychopathology and functioning based on Achenbach’s system, and measures of psychological well-being. Results revealed no association between mentalizing polarities and higher-order symptom factors. Self-mentalizing was associated with self-esteem and motivation to life goals, and other-mentalizing was associated to general, social and role functioning. This association between aspects of self-other mentalizing and self-other function has important implications for treatment and prevention. Deeper mentalizing within each polarity revealed stronger associations with functioning and well-being. Because mentalizing polarities are associated with functioning and well-being but not with symptoms, a new hypothesis is developed: mentalizing does not contribute to resiliency by preventing symptoms, but by helping to deal with them, thus improving functioning and well-being independently of psychopathology. These findings support that promoting mentalizing across development may improve mental health, even in non-clinical population. (shrink)

La psiquiatría nace con el siglo XIX, la fenomenología con el XX. Desde el advenimiento de la segunda ha querido la primera ver en ella una vía para la superación del positivismo que la atenazaba desde su mismo origen. Uno de sus intentos más logrados fue el del psiquiatra Ludwig Binswanger a lo largo de los años veinte del pasado siglo. Recorrió en ese tiempo las Investigaciones lógicas de Husserl y ensayó su acercamiento a la psicología estructural. Proponemos que una (...) deficiente lectura de las posibilidades de la fenomenología ―comprensible en su prematuridad, desgraciada en su ímpetu anti-(neo)kantiano― impidió que reconociera su horizonte trascendental y así pudiera entrever caminos de acceso al mundo, a la persona y a su concreto sufrimiento. Para concluir presentamos el intento que supone el proyecto La mirada filosófica como mirada médica por recuperar aquella problematicidad y ahondar en una aproximación respetuosa al padecer, tal como aparece, sin violentarlo por el concepto. (shrink)

Industrial shrimp farming has been promoted by international development and financial institutions in coastal indebted poor countries as a way to obtain foreign exchange earnings, reimburse external debt, and promote development. The promotion of the shrimp industry is a clear example of a more general trend of support of export-oriented primary products, consisting in monocultures of commodities, as opposed to the promotion of more diverse, traditional production directed to feed the local population. In general, it is assumed that export-oriented aquaculture (...) and agriculture, in a framework of liberalization policies, facilitates economic growth and this is associated with poverty reduction and the improvement of food security. However, it has been shown that the promotion of export-oriented production, mostly in the hands of big corporations, can have detrimental consequences for the livelihoods of local populations and the environment. As a result, international institutions, NGOs, and the industry aim to minimize these impacts by promoting sustainable export-oriented production. But some impacts may remain, since the main issue is the primary focus on international deregulated markets and the search for cheap primary products. To illustrate the relationships between the mainstream concept of development, the environmental and social impact of industrial farming systems, and the promotion of export-oriented production in developing countries, this article analyzes the case of the shrimp aquaculture industry. (shrink)

Graham Priest has influentially claimed that the Sorites paradox is an Inclosure paradox, concluding that his favored dialetheic solution to the Inclosure paradoxes should be extended to the Sorites paradox. We argue that, given Priest’s dialetheic solution to the Sorites paradox, the argument purporting to show that that paradox is an Inclosure is unsound, and discuss some issues surrounding this fact.

While a growing number of works have been published about the use of mixed methods research in nursing, scarce attention has been devoted to the issue of the quality of mixed methods within the discipline. The quality appraisal of mixed methods research poses two problems to nursing science: first, current quality criteria are not nursing‐specific and consequently, they might not facilitate the application of mixed methods research findings into nursing practice. Second, criteria were theoretically derived and as such, they might (...) not faithfully account for the decisions that nurse researchers take when appraising mixed methods research studies. This qualitative, within‐case study explored the views of nurse researchers about mixed methods research in general and, more specifically, the question of quality in mixed methods research. An international sample of 13 nurse researchers was interviewed via Skype and phone. Thematic analysis revealed that the participants favored universal, cross‐disciplinary quality criteria, and not criteria specific to the nursing discipline, and a consensual and standardized approach to appraising the quality of mixed methods research. Recommendations are put forward to strengthen the quality appraisal of mixed methods research studies by nurse researchers, as well as future works on this topic within nursing science. (shrink)

Asking “What is time?” can be both a simple and a profound question. In this article we intend to introduce the reader to the philosophy of time. To do so, we will deal with McTaggart's paradox. By explaining it and introducing the basic concepts to understand it, we will be able to get an idea of what this branch of philosophy is all about. The main intention of this article is not to explain anything new but to clarify the background (...) of a debate by explaining its roots. By taking a deep dive into the concept of time, we will see that it is not a simple concept at all. In any subsequent possible definition with regards to concepts such time for education or time for ethics, a logical clarification of what time is important and will color all further predication of the concept of time. Considering the impact of digital transformation in our lives in general, and in education in particular, a philosophy of time brings major elements such as paradoxes, which are not of practical character but important for the semantic of the words, such as “duration”, “temporal position” of events, etc. (shrink)

This essay is a revision of ?Rudiments of an Ethics of News Reporting,?; which won honorable mention in the 1985 Carol Burnett/University of Hawaii/ AEJMC Prize for Student Papers on Journalism Ethics. It argues that news reporting suffers from a misplaced faith in individual autonomy, a faith that resists a sense of social duty on the basis of negative freedom; therefore, journalism stands in need of a moral theory that recognizes community and personhood as fundamental human characteristics essential to ethical (...) decision?making. (shrink)

One of the main problems that Paul Horwich’s Minimalist theory of truth must face is the generalization problem, which shows that Minimalism is too weak to have the fundamental explanatory role Horwich claims it has. In this paper, I defend Horwich’s response to the generalization problem from an objection raised by Bradley Armour-Garb. I also argue that, given my response to Armour-Garb, Horwich’s proposal to cope with the generalization problem can be simplified. -/- L’un des principaux problèmes auxquels la théorie (...) minimaliste de la vérité de Paul Horwich doit faire face est le problème de la généralisation. Horwich soutient que le minimalisme a un rôle explicatif fondamental, mais le problème de la généralisation montre que cette théorie est trop faible pour tenir ce rôle. Dans cet article, je défends la réponse d’Horwich au problème de la généralisation à partir d’une objection soulevée par Bradley Armour-Garb. Je prétends également que ma réponse à Armour-Grab nous permet de formuler d’une manière plus simple la réponse d’Horwich au problème de la généralisation. (shrink)

In the last few decades, increasing attention has been given to gender issues in urban design. However, research on the urban environment continues to show large gender inequalities, which are especially evident when studying the use and enjoyment of the public space. This study aims to identify predominant patterns of use in public places and to explore the possible existence of traditional gender roles in the urban space. The study uses, three public spaces in the city of Barcelona as a (...) case study, an innovative combination of systematic observation techniques and network analysis procedures. Variables collected by EXOdES, a dedicated software analysis tool for systematic observation, are represented as nodes of a network system and analyzed using network analysis tools. Findings confirmed that, in spite of the progressive consolidation of feminist urbanism, uses in the public realm resulting from traditional gender roles remained explicitly recognizable. Whereas women’s occupation of space was related to playground and resting areas, generally involving care activities concerned with children or elderly people, men were primarily located in resting and sports areas, practicing sports, or participating in leisure activities. These patterns of use were more prone to emerge when users were part of a group than when they were alone. From a gender perspective, a contribution of the study is that it informed about main aspects of the analyzed public spaces reconfirming the existence of traditional roles in society, and the significance of exploring the public space as a key scenario where social features are explicitly exposed. From a methodological perspective, the processing of observational data with network analysis tools proved to be relevant and suitable for dealing with the intricacies of urban place analysis. Compared to more classical approaches and systems, these techniques allowed to identify and interpret complex systems composed of many variables and relationships in a relatively straightforward manner, which turns it into a useful aid for urban designers and architects. (shrink)

Mass media ethics and the classical liberal ideal of the autonomous individual are historically linked and professionally dominant--yet the authors of this work feel this is intrinsically flawed. They show how recent research in philosophy and social science--together with a longer tradition in theological inquiry--insist that community, mutuality, and relationship are fundamental to a full concept of personhood. The authors argue that "persons-in-community" provides a more defensible grounding for journalists' professional moral decision-making in crucial areas such as truthtelling, privacy, organizational (...) culture, and balanced coverage. With numerous examples drawn from life as well as from theory, this book will interest journalists, editors, and professionals in media management as well as students and scholars of media ethics, reporting, and media law. (shrink)

Meat?eating as a human practice has been under ethical attack from philosophers such as Peter Singer and Tom Regan on both utilitarian and deontological grounds. An organicist ethic, on the other hand, recognizes that all life other than the primary producers, the plants, must feed on life. This essay affirms, with many environmental ethicists, the moralconsiderability of biota other than the human, but denies that this enlargement of the moral community beyond Homo sapiens necessarily precludes our eating of meat. First, (...) absolute deontological arguments against meat?eating are disputed, then utilitarian?hedonistic arguments are shown not to be sufficient to require ethical vegetarianism. Both sorts of arguments have strengths, however, that set us on guard against current abuses in the meat?raising and slaughtering industries. If the principle of ?due respect? for beings with different degrees of intrinsic value is honored, then moderate meat?eating under reformed social practices can be seen as licit. Two final problems then require investigation: the problem of dietary justice for poor humans and the problem of ?speciesism?. Dealing with the latter requires discussion of cannibalism and the ethics of humans being eaten by still higher ?aliens? (shrink)

In this paper I introduce Horwich’s deflationary theory of truth, called ‘Minimalism’, and I present his proposal of how to cope with the Liar Paradox. The proposal proceeds by restricting the T-schema and, as a consequence of that, it needs a constructive specification of which instances of the T-schema are to be excluded from Minimalism. Horwich has presented, in an informal way, one construction that specifies the Minimalist theory. The main aim of the paper is to present and scrutinize some (...) formal versions of Horwich’s construction. (shrink)

Linguistic justice is about institutions distributing material and symbolic resources fairly when they are faced with linguistic diversity. However, no theory of linguistic justice has developed a systematic and comprehensive account of the moral dilemmas that take place in interpersonal linguistic relationships, in particular the power dynamics leading to (linguistic) domination. The aim of this article is to start building a general theory of linguistic domination, one that offers new conceptual tools for both empirical and normative analyses of linguistically diverse (...) societies. Using the republican tradition of thought, I argue that there is linguistic domination whenever someone is subject to the uncontrolled capacity for interference over their linguistic uses, ideology and acquisition by another agent. This article tests under what conditions this phenomenon takes place and the parties involved in it (both in terms of individuals and political institutions). (shrink)

This paper argues that the traditional characterization of the notion of paradox — an apparently valid argument with apparently true premises and an apparently false conclusion — is too narrow; there are paradoxes that do not satisfy it. After discussing, and discarding, some alternatives, an outline of a new characterization of the notion of paradox is presented. A paradox is found to be an apparently valid argument such that, apparently, it does not present the kind of commitment to the conclusion (...) that should be implied by an acceptance of the truth of the premises and the validity of the argument. (shrink)

Ce volume collectif montre que l'herméneutique fictionnalisée permet de réfléchir sur les procédures de l'interprétation, de penser les limites de la fiction et de prendre en charge des discours que les disciplines savantes ne sont pas toujours en situation de produire.

Two of the main intuitions that underlie the phenomenon of vagueness are the truth-functional and the penumbral intuitions. After presenting and contrasting them, I will put forward Tappenden's gappy approach to vagueness (which takes into account the truth-functional intuition). I will contrast Tappenden'sview with another of the theories of vagueness that see it as a semantic phenomenon: Supervaluationism (which takes into account the penumbral intuition). Then I will analyze some objections to Tappenden's approach and some objections to Supervaluationism. Finally, I (...) will present my own worries about Tappenden's account. (shrink)

In recent years, some authors have proposed quantitative measures of the coherence of sets of propositions. Such probabilistic measures of coherence (PMCs) are, in general terms, functions that take as their argument a set of propositions (along with some probability distribution) and yield as their value a number that is supposed to represent the degree of coherence of the set. In this paper, I introduce a minimal constraint on PMC theories, the weak stability principle, and show that any correct, coherent, (...) and complete PMC cannot satisfy it. As a matter of fact, the argument offered in this paper can be applied to any coherence theory that uses a priori procedures. I briefly explore some consequences of this fact. (shrink)