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Cell migration after synovium graft interposition at tendon repair site

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HAND

Abstract

Background

We have recently reported that interpositional synovium grafts from tendon sheath have a potential to accelerate tendon healing when implanted at the repair site. The purpose of this study was to investigate the effect of orientation of the synovium after synovium graft transplantation, by comparing the ability of cells from the visceral and parietal surfaces to migrate into the tendon in a canine tissue culture model.

Methods

The synovium graft was placed within a complete tendon laceration, with either the visceral or parietal surface facing the proximal end of the lacerated tendon. The number of migrating cells was quantified by a cell migration assay. Qualitative immunohistochemistry and confocal laser microscopy were also used at day 10.

Results

Many labeled synovial cells were observed within the tendon to which the visceral surface of the synovium graft was facing. Migrated cells were also observed on the parietal side, but there were fewer cells compared to visceral surface cells. Migrating cells all expressed α-smooth muscle actin.

Conclusion

We found that graft orientation affected cell migration. Whether this finding has clinical significance awaits in vivo study.

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Acknowledgments

This study was supported by grants from NIH (NIAMS) AR44391 and Mayo Foundation.

Conflicts of Interest

The authors declare they have no conflict of interest.

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Authors and Affiliations

  1. Biomechanics Laboratory, Division of Orthopedic Research, Mayo Clinic, 200 1st Street S.W., Rochester, MN, 55905, USA

    Masanori Hayashi, Chunfeng Zhao, Kai-Nan An & Peter C. Amadio

Authors
  1. Masanori Hayashi
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  2. Chunfeng Zhao
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  3. Kai-Nan An
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  4. Peter C. Amadio
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Corresponding author

Correspondence to Peter C. Amadio.

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Hayashi, M., Zhao, C., An, KN. et al. Cell migration after synovium graft interposition at tendon repair site. HAND 7, 374–379 (2012). https://doi.org/10.1007/s11552-012-9453-x

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  • DOI: https://doi.org/10.1007/s11552-012-9453-x

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