_In light of Herzberg's two-factor theory, the primary goal of this paper is to investigate the job satisfaction of the faculty members of private and public sector universities in Islamabad with specific reference to hygiene and motivation factors. For the purpose the sample contained 150 staff of private and public universities of Islamabad. The data collection was carried out through Minnesota Questionnaire as a tool of job satisfaction. The sampling techniques of proportional, stratified sampling was selected. The data was analyzed (...) through quantitative method by using the SPSS 21 st edition named as Statistical Package for social science. The results were reflected by using independent samples t-test. According to the study's findings the hygiene factor has significant impact on job satisfaction of participants with reference to Salary, Status, Work Conditions, Interpersonal Relationship with Subordinates and Possibility for Personal Growth besides motivational elements such as recognition and achievement. The data shows that high motivational level is found in public universities faculty member than the private sector universities._. (shrink)