In the past few decades, increased awareness of environmental pollution has led to the exploitation of microbial metabolic potential in the construction of several genetically engineered microorganisms (GEMs) for bioremediation purposes. At the same time, environmental concerns and regulatory constraints have limited the in situ application of GEMs, the ultimate objective behind their development. In order to address the anticipated risks due to the uncontrolled survival/dispersal of GEMs or recombinant plasmids into the environment, some attempts have been made to (...) construct systems that would contain the released organisms. This article discusses the designing of safer genetically engineered organisms for environmental release with specific emphasis on the use of bacterial plasmid addiction systems to limit their survival thus minimizing the anticipated risk. We also conceptualize a novel strategy to construct “Suicidal Genetically Engineered Microorganisms (SGEMs)” by exploring/combining the knowledge of different plasmid addiction systems (such as antisense RNA‐regulated plasmid addiction, proteic plasmid addiction etc.) and inducible degradative operons of bacteria. BioEssays 27:563–573, 2005. © 2005 Wiley Periodicals, Inc. (shrink)

In this essay, I explore non-human multispecies interactions in soils polluted by electronic waste and subsequently bioremediated by plants and microbes. I argue that regenerative transformation in polluted soil environments is principally through microbial degradation, a significant process for survival amidst disaster. In doing so, I combine two separate research areas – the materiality of electronic waste and of soils – thus contributing to theorization on the persistent problem of anthropogenically polluted soils. I do so by examining the process of (...) bioremediation, which ties anthropogenic pollution with underground soil processes, notably those that occur at the soil interface surrounding plant roots, the rhizosphere. Using empirical examples from scientific literature on the bioremediation of electronic waste-contaminated soils in China, I demonstrate that degradation, symbiosis, and sequestration are instrumental processes in polluted soils. The micro-scaled perspective of these relational processes and their toxic alterlives contributes to materialist, chemosocial understandings of toxic and polluted environments. (shrink)

In this article, I examine the rhetoric of democratic science within the field of synthetic biology. The still emerging field of synthetic biology claims to be a new kind of science based on the promises of affordable medicines, environmental bioremediation, and democratic, do-it-yourself science practices. I argue that the formation of a more democratic, DIY portion of this field represents an intervention into ethics debates by becoming “the proper informed public.” Through an analysis of twelve DIY and community-based synthetic (...) biology organizations’ websites, I found that democratic science was presented as a novel, progressive approach to science that addresses ethical concerns and at the same time produces better scientific results. In part, these claims were made possible through a reconfiguring of the boundaries between Science and the Public where scientists lay claim to solidarity with the public at large in opposition to traditional biosciences and Big Bio. My research suggests that the superficial use of the language of rights and democracy relegitimizes the primacy of scientific discovery to solve societal problems. I further suggest that by becoming the proper informed public, ethical challenges from publics critical of genetic sciences may become delegitimized. (shrink)

E-waste, also known as waste from electrical and electronic equipment, is a solid waste that accumulates quickly due to high demand driven by the market for replacing newer electrical and electronic products. The global e-waste generation is estimated to be between 53.6 million tons, and it is increasing by 3–5% per year. Metals make-up approximately 30% of e-waste, which contains precious elements Au, Ag, Cu, Pt, and other high-value elements, valued at USD 57 billion, which is driving the e-waste recycling (...) industry. It is noteworthy that the recycling of precious elements from e-waste has emerged as a profitable enterprise in several parts of developing nations. E-waste contains 50–100 times higher levels of precious metals compared to natural ores, making it suitable for mining. E-waste recycling in developing nations, mostly occurs through the informal sector comprising manual collection, crushing, segregation and selling of precious elements, such as Au, Ag, Cu, Pb, Pt, and other rare elements (Nd, In, and Ga). The organized sector, on the other hand, mostly employs mechano-chemical methods, such as pyrometallurgy, hydrometallurgy, and bio-hydrometallurgy, which have serious environmental consequences. Both the informal and formal sectors of e-waste processing lead to the leaching of toxic elements into groundwater and soils. Owing to the lesser efficiency of greener technologies, such as phytoremediation and bioremediation, their use in precious metal extraction is very limited. However, this review explores several hyper-accumulating and tolerant plants viz. Brassica juncea and Berkheya coddii, which holds great potential in phytomining of precious metal from e-waste. Thus, the state of the art in precious metal extraction from e-waste as well as the advantages and disadvantages of different metal extraction technologies has been reviewed. (shrink)