While mass spectrometry (MS)‐based quantification of small molecules has been successfully used for decades, targeted MS has only recently been used by the proteomics community to investigate clinical questions such as biomarker verification and validation. Targeted MS holds the promise of a paradigm shift in the quantitative determination of proteins. Nevertheless, targeted quantitative proteomics requires improvisation in making sample processing, instruments, and data analysis more accessible. In the backdrop of the genomic era reaching its zenith, certain questions arise: is the (...) proteomic era about to come? If we are at the beginning of a new future for protein quantification, are we prepared to incorporate targeted proteomics at the benchside for basic research and at the bedside for the good of patients? Here, an overview of the knowledge required to perform targeted proteomics as well as its applications is provided. A special emphasis is placed on upcoming areas such as peptidomics, proteoform research, and mass spectrometry imaging, where the utilization of targeted proteomics is expected to bring forth new avenues. The limitations associated with the acceptance of this technique for mainstream usage are also highlighted. (shrink)

I propose that metaphysical study is initially indifferent to the truth of Metaphysical Realism about Metaphysics (MRM) and Metaphysical Realism and does not presuppose them. Metaphysical Realism is a metaphysical doctrine the truth of which cannot be settled logically prior to metaphysical investigation. MRM presupposes Metaphysical Realism and therefore, one should not hold MRM uncritically. An epistemological consequence of this is that arguments against the possibility of cognition about metaphysically real entities (by e.g., Hume) are not arguments against the epistemic (...) legitimacy of metaphysics without further arguments for MRM. This paper is also a case study of the philosophical significance of having rich enough acquaintance with the history of philosophy, the recent history included. (shrink)