Purpose: The purpose of this article was to compare different modes of feedback on 30 min cycling time-trial performance in non-cyclist’s and cyclists-triathletes, and investigate cyclists-triathlete’s information acquisition.Methods: 20 participants performed two 30 min self-paced cycling time-trials with either a single feedback or multiple feedback. Cyclists-triathlete’s information acquisition was also monitored during the multiple feedback trial via an eye tracker. Perceptual measurements of task motivation, ratings of perceived exertion and affect were collected every 5 min. Performance variables and heart rate (...) were recorded continuously.Results: Cyclists-triathletes average power output was greater compared to non-cyclists with both multiple feedback. Whereas, cyclists-triathletes 30 min cycling time-trial performance was impaired with multiple feedback compared to single feedback. Cyclists-triathlete’s primary and secondary objects of regard were power and elapsed time. However, total glance time during multiple feedback decreased from the first 5 min to the last 5 min.Conclusion: Cyclists-triathletes indoor 30 min cycling TT performance was impaired with multiple feedback compared to single feedback. Whereas non-cyclist’s performance did not differ between multiple and single feedback. Cyclists-triathletes glanced at power and time which corresponds with the wireless sensor networks they use during training. However, total glance time during multiple feedback decreased over time, and therefore, overloading athletes with feedback may decrease performance in cyclists-triathletes. (shrink)