The objectives of this study were to develop a high-density chromosome bin map of homoeologous group 7 in hexaploid wheat, to identify gene distribution in these chromosomes, and to perform comparative studies of wheat with rice and barley. We mapped 2148 loci from 919 EST clones onto group 7 chromosomes of wheat. In the majority of cases the numbers of loci were significantly lower in the centromeric regions and tended to increase in the distal regions. The level of duplicated loci (...) in this group was 24% with most of these loci being localized toward the distal regions. One hundred nineteen EST probes that hybridized to three fragments and mapped to the three group 7 chromosomes were designated landmark probes and were used to construct a consensus homoeologous group 7 map. An additional 49 probes that mapped to 7AS, 7DS, and the ancestral translocated segment involving 7BS also were designated landmarks. Landmark probe orders and comparative maps of wheat, rice, and barley were produced on the basis of corresponding rice BAC/PAC and genetic markers that mapped on chromosomes 6 and 8 of rice. Identification of landmark ESTs and development of consensus maps may provide a framework of conserved coding regions predating the evolution of wheat genomes. (shrink)

A total of 1918 loci, detected by the hybridization of 938 expressed sequence tag unigenes from 26 Triticeae cDNA libraries, were mapped to wheat homoeologous group 4 chromosomes using a set of deletion, ditelosomic, and nulli-tetrasomic lines. The 1918 EST loci were not distributed uniformly among the three group 4 chromosomes; 41, 28, and 31% mapped to chromosomes 4A, 4B, and 4D, respectively. This pattern is in contrast to the cumulative results of EST mapping in all homoeologous groups, as reported (...) elsewhere, that found the highest proportion of loci mapped to the B genome. Sixty-five percent of these 1918 loci mapped to the long arms of homoeologous group 4 chromosomes, while 35% mapped to the short arms. The distal regions of chromosome arms showed higher numbers of loci than the proximal regions, with the exception of 4DL. This study confirmed the complex structure of chromosome 4A that contains two reciprocal translocations and two inversions, previously identified. An additional inversion in the centromeric region of 4A was revealed. A consensus map for homoeologous group 4 was developed from 119 ESTs unique to group 4. Forty-nine percent of these ESTs were found to be homoologous to sequences on rice chromosome 3, 12% had matches with sequences on other rice chromosomes, and 39% had no matches with rice sequences at all. Limited homology was found between wheat ESTs on homoeologous group 4 and the Arabidopsis genome. Forty-two percent of the homoeologous group 4 ESTs could be classified into functional categories on the basis of blastX searches against all protein databases. (shrink)

To localize wheat ESTs on chromosomes, 882 homoeologous group 6-specific ESTs were identified by physically mapping 7965 singletons from 37 cDNA libraries on 146 chromosome, arm, and sub-arm aneuploid and deletion stocks. The 882 ESTs were physically mapped to 25 regions flanked by 23 deletion breakpoints. Of the 5154 restriction fragments detected by 882 ESTs, 2043 were localized to group 6 chromosomes and 806 were mapped on other chromosome groups. The number of loci mapped was greatest on chromosome 6B and (...) least on 6D. The 264 ESTs that detected orthologous loci on all three homoeologs using one restriction enzyme were used to construct a consensus physical map. The physical distribution of ESTs was uneven on chromosomes with a tendency toward higher densities in the distal halves of chromosome arms. About 43% of the wheat group 6 ESTs identified rice homologs upon comparisons of genome sequences. Fifty-eight percent of these ESTs were present on rice chromosome 2 and the remaining were on other rice chromosomes. Even within the group 6 bins, rice chromosomal blocks identified by 1-6 wheat ESTs were homologous to up to 11 rice chromosomes. These rice-block contigs were used to resolve the order of wheat ESTs within each bin. (shrink)

Drawing on a landscape analysis of existing data-sharing initiatives, in-depth interviews with expert stakeholders, and public deliberations with community advisory panels across the U.S., we describe features of the evolving medical information commons. We identify participant-centricity and trustworthiness as the most important features of an MIC and discuss the implications for those seeking to create a sustainable, useful, and widely available collection of linked resources for research and other purposes.

The complex hexaploid wheat genome offers many challenges for genomics research. Expressed sequence tags facilitate the analysis of gene-coding regions and provide a rich source of molecular markers for mapping and comparison with model organisms. The objectives of this study were to construct a high-density EST chromosome bin map of wheat homoeologous group 2 chromosomes to determine the distribution of ESTs, construct a consensus map of group 2 ESTs, investigate synteny, examine patterns of duplication, and assess the colinearity with rice (...) of ESTs assigned to the group 2 consensus bin map. A total of 2600 loci generated from 1110 ESTs were mapped to group 2 chromosomes by Southern hybridization onto wheat aneuploid chromosome and deletion stocks. A consensus map was constructed of 552 ESTs mapping to more than one group 2 chromosome. Regions of high gene density in distal bins and low gene density in proximal bins were found. Two interstitial gene-rich islands flanked by relatively gene-poor regions on both the short and long arms and having good synteny with rice were discovered. The map locations of two ESTs indicated the possible presence of a small pericentric inversion on chromosome 2B. Wheat chromosome group 2 was shown to share syntenous blocks with rice chromosomes 4 and 7. (shrink)

This report describes the rationale, approaches, organization, and resource development leading to a large-scale deletion bin map of the hexaploid wheat genome. Accompanying reports in this issue detail results from chromosome bin-mapping of expressed sequence tags representing genes onto the seven homoeologous chromosome groups and a global analysis of the entire mapped wheat EST data set. Among the resources developed were the first extensive public wheat EST collection. Described are protocols for sequencing, sequence processing, EST nomenclature, and the assembly of (...) ESTs into contigs. These contigs plus singletons were used for selection of distinct sequence motif unigenes. Selected ESTs were rearrayed, validated by 5′ and 3′ sequencing, and amplified for probing a series of wheat aneuploid and deletion stocks. Images and data for all Southern hybridizations were deposited in databases and were used by the coordinators for each of the seven homoeologous chromosome groups to validate the mapping results. Results from this project have established the foundation for future developments in wheat genomics. (shrink)

The focus of this study was to analyze the content, distribution, and comparative genome relationships of 996 chromosome bin-mapped expressed sequence tags accounting for 2266 restriction fragments on the homoeologous group 3 chromosomes of hexaploid wheat. Of these loci, 634, 884, and 748 were mapped on chromosomes 3A, 3B, and 3D, respectively. The individual chromosome bin maps revealed bins with a high density of mapped ESTs in the distal region and bins of low density in the proximal region of the (...) chromosome arms, with the exception of 3DS and 3DL. These distributions were more localized on the higher-resolution group 3 consensus map with intermediate regions of high-mapped-EST density on both chromosome arms. Gene ontology classification of mapped ESTs was not significantly different for homoeologous group 3 chromosomes compared to the other groups. A combined analysis of the individual bin maps using 537 of the mapped ESTs revealed rearrangements between the group 3 chromosomes. Approximately 232 of the consensus mapped ESTs matched sequences on rice chromosome 1 and revealed large- and small-scale differences in gene order. Of the group 3 mapped EST unigenes ∼21 and 32% matched the Arabidopsis coding regions and proteins, respectively, but no chromosome-level gene order conservation was detected. (shrink)

A total of 944 expressed sequence tags generated 2212 EST loci mapped to homoeologous group 1 chromosomes in hexaploid wheat. EST deletion maps and the consensus map of group 1 chromosomes were constructed to show EST distribution. EST loci were unevenly distributed among chromosomes 1A, 1B, and ID with 660, 826, and 726, respectively. The number of EST loci was greater on the long arms than on the short arms for all three chromosomes. The distribution of ESTs along chromosome arms (...) was nonrandom with EST clusters occurring in the distal regions of short arms and middle regions of long arms. Duplications of group 1 ESTs in other homoeologous groups occurred at a rate of 35.5%. Seventy-five percent of wheat chromosome 1 ESTs had significant matches with rice sequences, where large regions of conservation occurred between wheat consensus chromosome 1 and rice chromosome 5 and between the proximal portion of the long arm of wheat consensus chromosome 1 and rice chromosome 10. Only 9.5% of group 1 ESTs showed significant matches to Arabidopsis genome sequences. The results presented are useful for gene mapping and evolutionary and comparative genomics of grasses. (shrink)

© 2015, Springer-Verlag Berlin Heidelberg.Genetic studies have identified single nucleotide polymorphisms associated with the risk of prostate cancer. It remains unclear whether such genetic variants are associated with disease aggressiveness. The NCI-SPORE Genetics Working Group retrospectively collected clinicopathologic information and genotype data for 36 SNPs which at the time had been validated to be associated with PC risk from 25,674 cases with PC. Cases were grouped according to race, Gleason score and aggressiveness. Statistical analyses were used to compare the frequency (...) of the SNPs between different disease cohorts. After adjusting for multiple testing, only PC-risk SNP rs2735839 was significantly and inversely associated with aggressive and high-grade disease in European men. Similar associations with aggressive and high-grade disease were documented in African-American subjects. The G allele of rs2735839 was associated with disease aggressiveness even at low PSA levels in both European and African-American men. Our results provide further support that a PC-risk SNP rs2735839 near the KLK3 gene on chromosome 19q13 may be associated with aggressive and high-grade PC. Future prospectively designed, case-case GWAS are needed to identify additional SNPs associated with PC aggressiveness. (shrink)

I. Wesen und entwicklung des humanitätsgedankens.--II. Der socialismus.--III. Der individualismus.--IV. Der utilitarianismus.--V. Der pessimismus.--VI. Der evolutionismus.--VII. Der positivismus.--VIII. Nietzsches uebermensch.--IX. Die hauptgrundsätze einer modernen humanitätsethik.

Издание содержит: познание в контексте культуры; эпистемология социально-гуманитарного знания; история философии: опыт познания культуры и др.

The supposed difficulties of this famous passage are set forth in Conington's notes. In reality they have been created by a misunderstanding, and chiefly through forgetfulness that the English of pauci is ' only a few.' In vv. 743–747 the sense is not that the souls dwell in Elysium ' until lapse of time hath removed the ingrown corruption.' This would surely require donee … exemerit …purumque reliquerit.The fact is that Anchises is explaining his own presence in Elysium at so (...) early a date after his death. If souls require a prolonged purgation before reaching Elysium and the laeta arua, how comes it that he himself is already there? The answer is—because he is one of the few in whose case the process was a short one.Quisque suos patimur Manes, says he, and the length and nature of the purgation depend upon what your Manes deserve and require.?‘ All souls go through such punishment and refining as are necessary.We each suffer our own Manes, and only a few of us are so fortunate as to enjoy the happy fields before a long period has removed the impurities.’ He is himself one of those who have so escaped, and hence his presence here to meet Aeneas so soon after decease. (shrink)

At all times, the book was understood not only as a means of preserving and transforming knowledge, but also as a means of knowing the world around us. At the same time, from ancient times it was a subject of knowledge. Gradually its theoretical phenomenon was formed. The book essentially is the most important form of consolidation and transfer of information in space and time. From the point of view of the theory of communication, the book serves as one of (...) the forms of existence and dissemination of semantic information, a means of organizing the work of individual consciousness into a sign system for the perception of its social consciousness. After all, the evolution of the book is inextricably linked with the history of mankind. She is the foundation on which the culture of peoples is built. It helps society to grow and improve, borrow and use all the mass of knowledge accumulated by mankind. The book is the most complete and comprehensive expression of the spiritual culture of mankind, since its origin and development are inextricably linked with it. (shrink)

The study of the formation of the literary culture of the words of the "peoples of the nations" is impossible without analyzing the role of libraries of monasteries and cathedrals as centers of documentary memory of the Christian past. After all, the library, as a social institution, has always played an important role in the development of education, science, culture, and religious thought on a long path to its development.

The great achievement of mankind was the appearance of a printed book that not only significantly expanded the circle of readers, but also in comparison with the handwritten book contributed to the unification of canonical texts, in particular, such as Scripture, church service books, works of the Church Fathers, polemical and other religious literature. Consideration of the words "Japanese typography as the basis for the preservation and transmission of sources of Christian literary culture requires a brief description of the essence (...) of printing, not only as a means of mechanical reproduction of the text, but also a remarkable cultural-historical phenomenon. (shrink)

This work is an introductory textbook for deductive logic being primarily concerned with truth-functional logic, but also containing an introduction to syllogisms with the application of Venn diagrams, an introduction to quantification theory, and a brief discussion of axiom systems. Harrison employs six logical operators in his truth-functional calculus, including both inclusive and exclusive disjunction. The six operators are initially defined by truth tables, but in the natural deduction presentation negation and conjunction are taken as primitive and the other connectives (...) are defined in terms of these two. The conditional and indirect methods of proof are included with the approach being essentially the same as that given in Copi’s Symbolic Logic. Categorical statements and syllogisms are analyzed from both hypothetical and existential viewpoints. The treatment of quantification theory includes two-place predicates and employs the four standard rules for generalization and instantiation. The book contains an abundance of explanations, examples, and exercises. Selected answers, usually for the odd numbered problems, are given in an appendix.—T. G. N. (shrink)

This volume offers to the English-speaking world a collection of important works by the eminent twentieth century logician, Jan Lukasiewicz, many of which are here translated into English for the first time. This edition differs significantly from the Polish edition which appeared in 1961—containing ten logic papers not appearing there and omitting articles primarily of interest to the Polish reader. In addition to writing in Polish, Lukasiewicz also published works in French, English, and notably in German, and sometimes translated his (...) own works from one language to another. One of the most valuable works on the history of logic is Lukasiewicz’ paper, "On the History of the Logic of Propositions". Lukasiewicz points out the difference between the two basic areas of formal logic, the logic of propositions and the logic of terms, undifferentiated before the development of modern mathematical logic. The understanding of this distinction leads Lukasiewicz to trace the history of propositional logic back to its original development by the Stoics, its further development by the medieval Scholastics, and its axiomatization by Gottlob Frege. The status of mathematical logic is discussed in various works. The most basic system is propositional logic, upon which depend the other logical disciplines and also mathematics. Mathematical logic, also called logistic, is independent of philosophy and espouses no philosophical viewpoint. Early in his career, Lukasiewicz refers to mathematical logic as the logic of algebra and uses mathematical symbolism to represent logical propositions. Later he introduces what we now call "Polish notation," in particular, "Cpq" for "if p, then q" and "Np" for "it is not the case that p" for the primitive functions, eliminating the need for punctuation. He adopts the symbols Π and Σ for quantification from Charles S. Peirce, and also brings to light the almost unknown fact that it was Peirce who invented the matrix method in 1885. The paper, "Investigations into the Sentential Calculus", written by Lukasiewicz and Tarski embodies the results of a decade of research on the sentential calculus initiated by Lukasiewicz at the University of Warsaw systematically compiling the contributions of five logicians: Lindenbaum, Sobocinski, Wajsberg, Tarski, and Lukasiewicz. The approach is metalogical and depends heavily upon set theoretic concepts; it covers both the matrix and axiomatic methods. One of the problems that preoccupied Lukasiewicz was that of determinism, which led to his development of three-valued logic. A proposition such as "I shall be in Warsaw at noon on 21 December of next year" is neither true nor false; a third truth value is needed, one which Lukasiewicz calls "the possible." Another area to which Lukasiewicz contributed is modal logic, proving that modal logic cannot be two-valued. Lukasiewicz’ analysis and axiomatization of Aristotle’s syllogistic is not included in the present volume since an English edition by Lukasiewicz on this topic is available. We have here briefly touched upon a few of Lukasiewicz’ numerous achievements in logic; the best means of appreciating them is to read his works.—T. G. N. (shrink)