Tissues contain complex populations of cells. Like countries, which are comprised of mixed populations of people, tissues are not homogeneous. Gene expression studies that analyze entire populations of cells from tissues as a mixture are blind to this diversity. Thus, critical information is lost when studying samples rich in specialized but diverse cells such as tumors, iPS colonies, or brain tissue. High throughput methods are needed to address, model and understand the constitutive and stochastic differences between individual cells. Here, we (...) describe microfluidics technologies that utilize a combination of molecular biology and miniaturized labs on chips to study gene expression at the single cell level. We discuss how the characterization of the transcriptome of each cell in a sample will open a new field in gene expression analysis, population transcriptomics, that will change the academic and biomedical analysis of complex samples by defining them as quantified populations of single cells. (shrink)

This work has been selected by scholars as being culturally important and is part of the knowledge base of civilization as we know it. This work is in the public domain in the United States of America, and possibly other nations. Within the United States, you may freely copy and distribute this work, as no entity has a copyright on the body of the work. Scholars believe, and we concur, that this work is important enough to be preserved, reproduced, and (...) made generally available to the public. To ensure a quality reading experience, this work has been proofread and republished using a format that seamlessly blends the original graphical elements with text in an easy-to-read typeface. We appreciate your support of the preservation process, and thank you for being an important part of keeping this knowledge alive and relevant. (shrink)

This paper considers the efficiency of the political market in the California State legislature. I analyzed the property tax limitation voter initiative, Proposition 13. I found that districts which supported Proposition 13 more strongly were more likely to oppose the incumbents regardless of whether the incumbents had the different preferences for property taxes from their districts. I also studied how legislators voted on the bills adopted after the passage of Proposition 13 to finance local governments. I found that legislators tended (...) to follow the constituents tax-cutting message expressed by the passage of Proposition 13. (shrink)

This paper considers a monthly pattern in government spending. I have found that public spending increases at the end of the fiscal year for both the Japanese central government and the US federal government and that the effects are stronger in recent years than in the past. I then propose two hypotheses that would explain why public spending increases at the end of the fiscal year.

This paper explores preferences and attitudes related to fiscal federalism held by the ASEAN people in the context of environmental issues. Fiscal federalism would predict that local environmental problems will be handled more efficiently by local governments, while national environmental problems will be solved more efficiently by the national government. But it is not obvious whether citizens consider in the same way as economics theory predicts. To unveil this point, I address questions of whether those who have higher consciousness toward (...) environmental issues at the neighbor or local level prefer local governments to decide environment policies, whether those who have more consciousness about environmental issues at the national level prefer the national government to decide the policies, and whether those who have higher consciousness toward environmental problems at global level prefer higher levels government such as the United Nations to decide the policies. By fitting multi-level probit regressions to cross-national survey data collected in ASEAN countries, I found the results supporting the hypotheses. The country analyses show the results which support the hypotheses in Brunei, Indonesia, and the Philippines. (shrink)

唐招提寺、薬師寺、法隆寺から、世界の名建築を経めぐり、そして桂離宮へ――。知られざる和辻倫理学のもうひとつの思想的源泉!

The apologetic method of the Nyāya is inductive. The subject is distilled from the scripture and the Nyāya investigates it through logical argument. Through this procedure of partial verification, the reliability of the composer is established, and consequently the authority of the entire scripture is justified. The domains of thescripture and rational investigation overlap in significant issues such as the reality of the Self, and therefore, they can cooperate to establish the common truth. In Mīmāṃsā apologetics, the domain of the (...) scripture and that of rational argument are mutually exclusive. Their apologetic program is basically accomplished as a process of purely rational investigation without referring to the content of the scripture. But at the same time, the authority of the Veda is regarded as innate and has no external basis. Working autonomously, the rational investigation imposes its own limitation from within and thereby secures the scriptural domain that is free from the intervention of any other kind of knowledge. The overlap between the domains of the scripture and the ordinary experience including rational investigation constitutes a necessary condition for the Nyāya apologetics, whereas in Mīmāṃsā apologetics, it is the absence of the overlapping domain that assures the unique authority of the Veda. This difference is supposed to be rooted in their disagreement on whether a man is able, or qualified, to examine the content of the scripture or not. (shrink)

江戸時代中期に開学した漢学塾懐徳堂。中井竹山・履軒兄弟が展開した懐徳堂儒学と朱子学との関係、思想史的意義を明らかにする。.

This paper generalizes the simple regret model by Bell in Operations Research 30(5), 961-981 and Loomes and Sugden in The Economic Journal 92(368), 805-824 to cope with the situation in which decision outcomes are multi-attributed. We propose a model that combines the simple regret model for ex ante preferences and the additive difference representation for ex post preferences. We first present a necessary and sufficient axiomatization of our model in Savage’s framework. The proposed model is composed of three types of (...) functions. One is a value function for each attribute. The others are attribute-dependent and holistic regret-rejoicing functions that capture trade-offs among value-differences of chosen and forgone outcomes for each attribute and among all attributes, respectively. We then provide constructive procedures for those functions and methods for consistency checks of the procedures. We, finally, conduct an experiment to estimate those functions in the two attributes case by means of the constructive procedure and give an application of the model and our findings. (shrink)