Baculovirus vectors have proven useful in producing high levels of biologically active eukaryotic proteins and providing cellular fractions which are enriched in the protein of interest. Expression occurs in infected insect cells which also provide a suitable environment for posttranslational modification and folding of the protein product. Stable baculovirus vectors can be constructed rapidly with a minimum of viral manipulation.

Alexithymia is a subclinical trait involving difficulty describing and identifying emotions. It is common in a number of psychiatric conditions. Alexithymia in children is sometimes measured by parent report and sometimes by child self-report, but it is not yet known how closely related the two measures are. This is an important question both theoretically and practically, in terms of research design and clinical practice. We conducted a preliminary study to investigate this question in a sample of 6- to 11-year-old neurotypical (...) children and their parents. Parent and child reports were not correlated, and 93% of parents under-estimated their child’s level of alexithymia relative to the child’s self-report. Based on these results, we hypothesize that when asked to report on the child’s alexithymia, children and parents may not be reporting on the same phenomenon, and thus these two measures may not be interchangeable. These provocative findings, however, must be considered preliminary: our analyses were sufficiently powered to detect a strong relation between the two types of report had one existed, but our analyses were not sufficiently powered to distinguish between a small relation and no relation at all. (shrink)

The aim of this paper is to develop an adequate version of the D-N theory of explanation for particular events and to show how the resulting D-N model can be used as a tool in articulating a regularity theory of causation and an analysis of the truth conditions for counterfactual conditionals. Starting with a basic model that is largely the product of other workers in this field, two new restrictions are formulated in order to construct a version of D-N explanation (...) that does not yield the counterintuitive results that have plagued all earlier versions. An additional condition is then developed that is indispensable for utilizing the D-N model as a tool in formulating a regularity theory of causation. Finally, it is shown how a suitable model of potential D-N explanation facilitates the formulation of a theory of counterfactual conditionals. (shrink)

The article attempts to resolve the problem raised by richard sharvey's arguments supporting the thesis that temporal contexts present difficulties for quantification similar to those presented by alethic modal contexts. to this end a concept of temporally relativized identity for physical objects is introduced and explicated within a four-dimensional space-time view of the world. this in turn permits the introduction of the notion of "restricted" physical objects and the notion of "extended" physical objects. it is then shown how the use (...) of the introduced concepts together with careful attention to the details of temporal predication may permit the solution of the problem. (shrink)

The proposal regarding rules and similarity is considered in terms of ability to provide insights regarding previous work on reasoning and categorization. For reasoning, the issue is the relation between this proposal and one-process as well as two-process accounts of deduction and induction. For categorization, the issue is how the proposal would simultaneously explain both similarity-to-rule and rule-to-similarity shifts.

It is widely agreed that ‘scientific law’ is one of the key scientific terms which any adequate philosophy of science must attempt to clarify or define. The importance of the concept ‘law’ is made evident by the fact that the distinctive functions of science—explanation and prediction—are usually analyzed with reference to laws. Thus events are explained by showing that descriptions of them are deducible from laws, and laws are utilized in deducing descriptions of unknown future events, thereby permitting their prediction. (...) Moreover, it has recently become clear that the concept ‘law’ is relevant to the analysis of counterfactual conditionals, disposition terms, and the physical modalities. An adequate explication of ‘law’, then, will further the analysis of these important concepts. (shrink)

Habermas's paradigm of communicative action is usually taken to be pretty much dominated by consensus, "Yes-saying." What if this were a radically one-sided perception? We take up this unorthodox position by arguing that "no-saying" in this paradigm is typically overlooked and underemphasized. To demonstrate this, we consider how negativity is figured at the most basic onto-ethical level in communicative action, as well as expressed in civil disobedience, a phenomenon to which Habermas assigns the remarkable role of "touchstone" (Prufstein) of constitutional (...) democracy. Once the importance of no-saying is drawn out, the paradigm looks distinctly less hostile to dissensus and agonism in democratic life. (shrink)

In humanities departments across the country Allan Bloom is condemned and reviled—but for what misconduct? The present investigation into our distorted view of Bloom begins by reframing his transgressions. Bloom has offended eminent philosophy professors by boosting students' desire for a philosophic education. He has emboldened promising young people by teaching them to bear up under the deforming forces of convention and corruption and edge their way towards the question, ‘How should I live my life?’ A philosophic education as Bloom (...) sees it ‘means precisely helping students to pose this question to themselves, to become aware that the answer is neither obvious nor simply unavailable, and that there is no serious life in which this question is not a continuous concern’. The philosopher's interests are not shared by most people. Hostility to philosophy is the natural condition of man. This surprising fact, plainly stated, invites so much anger that Bloom—standing in for the philosopher—is forced to defend himself. The story of Bloom, like Socrates', gives an account of philosophy's emergence, distinctive features and always imaginable suppression. Plato's artful writing captured Socrates' way of life for posterity. This essay explores the nature and function of Bloom's bestselling book The Closing of the American Mind (1987), as well as the purpose of Saul Bellow's final novel, Ravelstein (2000), a fictive account of Bloom's life and death. Together these books amount to the true Apology of Allan Bloom. (shrink)

The literary sources for the Flavian and Antonine periods of Roman history, it is a notorious and unhappy fact, where they exist at all, are infuriatingly fragmentary, frequently obscure, too frequently inaccurate or mendacious. Significant gaps still linger even in chronology; hence it can hardly occasion surprise that we are rarely permitted a glimpse of the political activity which preoccupied the emperors and Senate.

In every generation the overwhelming majority of those who inhabited the imperium Romanum worked on the land and derived their sustenance directly from it. The notion is commonplace and scarcely admits of debate, but its implications for long have suffered unwarranted neglect. The well-being of any society ultimately rests upon the quantity and diversity of its food supplies, but the immediacy of their contact with the soil continually reminded the Roman people of this platitude with a force which few students (...) of their history today can readily appreciate. The annual yield of wheat in particular, always a staple item in the Roman diet, and to a lesser extent of such cereals as panic and millet, acutely affected every segment of the Roman community. One example will serve to illustrate the point. The dependence of the plebs urbana upon imported surpluses is notorious, equally the intense suffering and violence which typically accompanied any disruption of their supply. Such outbreaks frequently punctuated the chaotic final decades of the Republic, but they also scarred more than one régime during the first two centuries of the Principate. (shrink)

Vaccine hesitancy is a major public health challenge, and racial disparities in the acceptance of vaccines is a particular concern. In this essay, we draw on interviews with mothers of Black male adolescents to offer insights into the reasons for the low rate of vaccination against the human papillomavirus among this group of adolescents. Based on these conversations, we argue that increasing the acceptance of HPV and other vaccines cannot be accomplished merely by providing people with more facts. Instead, we (...) must address the pervasive racial discrimination in the United States that undermines trust in social institutions, including the health care system. In the short term, it may be helpful to increase the number of clinicians of color working in the health system, but more fundamental changes are required. The U.S. must adopt and implement policies that dismantle structural racism if it hopes to produce greater trust and community‐oriented thinking on behalf of people who have been exploited for centuries. (shrink)

Philosophical Investigations, EarlyView.