Eukaryotic genome DNA is wrapped around core histones and forms a nucleosome structure. Together with associated proteins and RNAs, these nucleosomes are organized three‐dimensionally in the cell as chromatin. Emerging evidence demonstrates that chromatin consists of rather irregular and variable nucleosome arrangements without the regular fiber structure and that its dynamic behavior plays a critical role in regulating various genome functions. Single‐nucleosome imaging is a promising method to investigate chromatin behavior in living cells. It reveals local (...) chromatin motion, which reflects chromatin organization not observed in chemically fixed cells. The motion data is like a gold mine. Data analyses from many aspects bring us more and more information that contributes to better understanding of genome functions. In this review article, we describe imaging of single‐nucleosomes and their tracked behavior through oblique illumination microscopy. We also discuss applications of this technique, especially in elucidating nucleolar organization in living cells. (shrink)

DNA is packed as chromatin on several levels in the eukaryotic nucleus. Dissection of chromatin with nucleases produces three stable substructures: the nucleosome core particle, the chromatosome and the 30 nm fibre. Whilst the first two allow transcription, the 30 nm fibre is taken to be the first level of transcriptionally dormant chromatin and it has an important functional role in cell differentiation and epigenetic regulation. Its structure has been a subject of continuing discussion since native fibres (...) cannot readily be crystallized. This problem has recently been addressed by reconstitution of fibres on repeats of DNA sequences having nucleosome‐positioning properties and two different structures were reported.1,2The reconstitution results and their interpretations are compared in this review with experimental data from native chromatin and it is shown that the results of Robinson et al.2 conform well with the known structural features of native fibres and are a good first step towards understanding the structure of the fibre. BioEssays 30:1003–1009, 2008. © 2008 Wiley Periodicals, Inc. (shrink)

We present a molecular model of eukaryotic gene transcription. For the β‐globin locus, we hypothesise that a transcription machine composed of multiple RNA polymerase II (PolII) assembles using the locus control region as a foundation. Transcription and locus remodelling can be achieved by pulling DNA through this multi‐PolII ‘reading head’. Once a transcription complex is formed, it may engage an active gene in several rounds of transcription. Observed intergenic sense and antisense transcripts may be the result of PolII pulling the (...) DNA through the reading head whilst searching for the promoter of a gene. Support for this hypothesis is provided using various data from the literature. In the model, DNA is packed in a 30‐nm chromatin fibre, thus gene regulatory regions separated by kilobases are close in space. This, and the need to store transcription‐induced supercoiling, may explain why functionally interacting regions are often separated by many kilobases. (shrink)

We present a molecular model of eukaryotic gene transcription. For the β‐globin locus, we hypothesise that a transcription machine composed of multiple RNA polymerase II (PolII) assembles using the locus control region as a foundation. Transcription and locus remodelling can be achieved by pulling DNA through this multi‐PolII ‘reading head’. Once a transcription complex is formed, it may engage an active gene in several rounds of transcription. Observed intergenic sense and antisense transcripts may be the result of PolII pulling the (...) DNA through the reading head whilst searching for the promoter of a gene. Support for this hypothesis is provided using various data from the literature. In the model, DNA is packed in a 30‐nm chromatin fibre, thus gene regulatory regions separated by kilobases are close in space. This, and the need to store transcription‐induced supercoiling, may explain why functionally interacting regions are often separated by many kilobases. (shrink)

Extensive regions of chromosomes can be transcriptionally repressed through silencing mechanisms mediated by complex chromatin structures. One of the most refined molecular portraits of silenced chromatin comes from studies of the silent mating‐type loci and telomeres of S. cerevisiae. In this budding yeast, the Sir3p silent information regulator emerges as a critically important silencing component that interacts with nucleosomes and other silencing proteins. Not only is it essential for silencing, but Sir3p is also capable of spreading silenced (...) class='Hi'>chromatin when its dosage is increased. Sir3p is a target of mitogen‐activated protein (MAP) kinase cascade regulation and has significant similarity to the Orc1p subunit of the DNA replication origin recognition complex. Thus, in concert with other silencing proteins, Sir3p appears poised to respond to cellular signals and reprogram silencing through replication‐associated assembly of repressive chromatin structures. BioEssays 20:30–40, 1998. © 1998 John Wiley & Sons, Inc. -/- . (shrink)

The recent surge of discoveries concerning the structural organization of nucleosomes, together with genetic evidence of highly specialized roles for the histones in gene regulation, have brought a renewed need for a detailed understanding of nucleosomal anatomy. Here we review recent structural advances leading to a new level of understanding of the nucleosome and chromatin fibre structure. We discuss the problems and challenges for existing models of chromatin structure and, in particular, consider how linker histones may bind within (...) the nucleosome, together with the implications of their association for the structure of the chromatin fibre. (shrink)

The various models of chromatin fiber folding that have been proposed over the years are considered and evaluated. It is concluded that the radial loop / scaffold model is strongly supported by the available evidence, although the term ‘scaffold’ may be an unfortunate one. The scaffold is not a solid rod running the length of the chromatid but rather appears to be an aggregation of discrete anchoring complexes for the loops of the fiber. Despite support for this (...) model, there is a need for more evidence to resolve the questions that remain. (shrink)

Insulators play a central role in subdividing the chromosome into a series of discrete topologically independent domains and in ensuring that enhancers and silencers contact their appropriate target genes. In this review we first discuss the general characteristics of insulator elements and their associated protein factors. A growing collection of insulator proteins have been identified including a family of proteins whose expression is developmentally regulated. We next consider several unexpected discoveries that require us to completely rethink how insulators function (and (...) how they can best be assayed). These discoveries also require a reevaluation of how insulators might restrict or orchestrate (by preventing or promoting) interactions between regulatory elements and their target genes. We conclude by connecting these new insights into the mechanisms of insulator action to dynamic changes in the three‐dimensional topology of the chromatin fiber and the generation of specific patterns of gene activity during development and differentiation. (shrink)

Synaptic ribbons, the organelles identified in electron micrographs of the sensory synapses involved in vision, hearing, and balance, have long been hypothesized to play an important role in regulating presynaptic function because they associate with synaptic vesicles at the active zone. Their physiology and molecular composition have, however, remained largely unknown. Recently, a series of elegant studies spurred by technical innovation have finally begun to shed light on the ultrastructure and function of ribbon synapses. Electrical capacitance measurements have provided sub‐millisecond (...) resolution of exocytosis, evanescent‐wave microscopy has filmed the fusion of single 30 nm synaptic vesicles, electron tomography has revealed the 3D architecture of the synapse, and molecular cloning has begun to identify the proteins that make up ribbons. These results are consistent with the ribbon serving as a vesicle “conveyor belt” to resupply the active zone, and with the suggestion that ribbon and conventional chemical synapses have much in common. BioEssays 23:831–840, 2001. © 2001 John Wiley & Sons, Inc. (shrink)

Three‐dimensional structured illumination microscopy (3D‐SIM) has opened up new possibilities to study nuclear architecture at the ultrastructural level down to the ∼100 nm range. We present first results and assess the potential using 3D‐SIM in combination with 3D fluorescence in situ hybridization (3D‐FISH) for the topographical analysis of defined nuclear targets. Our study also deals with the concern that artifacts produced by FISH may counteract the gain in resolution. We address the topography of DAPI‐stained DNA in nuclei before and after (...) 3D‐FISH, nuclear pores and the lamina, chromosome territories, chromatin domains, and individual gene loci. We also look at the replication patterns of chromocenters and the topographical relationship ofXist‐RNA within the inactive X‐territory. These examples demonstrate that an appropriately adapted 3D‐FISH/3D‐SIM approach preserves key characteristics of the nuclear ultrastructure and that the gain in information obtained by 3D‐SIM yields new insights into the functional nuclear organization. (shrink)

Local differences in chromatin organisation may profoundly affect the activity of eukaryotic genomes. Regulation at the level of DNA packaging requires the targeting of structural proteins and histone‐modifying enzymes to specific sites and their stable or dynamic interaction with the nucleosomal fiber. The “chromodomain”, a domain shared by many regulators of chromatin structure, has long been suspected to serve as a module mediating chromatin interactions in a variety of different protein contexts. However, recent functional analyses of (...) a number of different chromodomains revealed an unexpected diversity of interaction targets, including histones, DNA and even RNA. The chromodomains of today seem to have evolved from a common ancestral fold to fulfill various functions in different molecular contexts. Combining information gained from recent functional and structural studies of chromodomains with a bioinformatic classification of their structure could lead to the definition of sequence motifs with predictive quality for chromodomain function. BioEssays 26:133–140, 2004. © 2004 Wiley Periodicals, Inc. (shrink)

Steroid‐hormone‐activated receptor proteins are among the best‐understood class of factors for altering gene transcription in cells. Steroid receptors are of major importance in maintaining normal human physiology by responding to circulating concentrations of steroid in the nM range. Nonetheless, most studies of steroid receptor action have been conducted using the supra‐physiological conditions of saturating concentrations (≥100 nM) of potent synthetic steroid agonists. Here we summarize the recent developments arising from experiments using two clinically relevant conditions: subsaturating concentrations of agonist (to (...) mimic the circulating concentrations in mammals) and saturating concentrations of antagonists (which are employed in endocrine therapies to block the actions of endogenous steroids). These studies have revealed new facets of steroid hormone action that could not be uncovered by conventional experiments with saturating concentrations of agonist steroids, such as a plethora of factors/conditions for the differential control of gene expression by physiological levels of steroid, a rational approach for examining the gene‐specific variations in partial agonist activity of antisteroids, and a dissociation of steroid potency and efficacy that implies the existence of separate, and possibly novel, mechanistic steps and cofactors. BioEssays 30:744–756, 2008. © 2008 Wiley Periodicals, Inc. (shrink)

Protein α-helices provide an ordered biological environment that is conducive to soliton-assisted energy transport. The nonlinear interaction between amide I excitons and phonon deformations induced in the hydrogen-bonded lattice of peptide groups leads to self-trapping of the amide I energy, thereby creating a localized quasiparticle (soliton) that persists at zero temperature. The presence of thermal noise, however, could destabilize the protein soliton and dissipate its energy within a finite lifetime. In this work, we have computationally solved the system of stochastic (...) differential equations that govern the quantum dynamics of protein solitons at physiological temperature, T=310 K, for either a single isolated α-helix spine of hydrogen bonded peptide groups or the full protein α-helix comprised of three parallel α-helix spines. The simulated stochastic dynamics revealed that although the thermal noise is detrimental for the single isolated α-helix spine, the cooperative action of three amide I exciton quanta in the full protein α-helix ensures soliton lifetime of over 30 ps, during which the amide I energy could be transported along the entire extent of an 18-nm-long α-helix. Thus, macromolecular protein complexes, which are built up of protein α-helices could harness soliton-assisted energy transport at physiological temperature. Because the hydrolysis of a single adenosine triphosphate molecule is able to initiate three amide I exciton quanta, it is feasible that multiquantal protein solitons subserve a variety of specialized physiological functions in living systems. (shrink)

Cohesin establishes sister‐chromatid cohesion during S phase to ensure proper chromosome segregation in mitosis. It also facilitates postreplicative homologous recombination repair of DNA double‐strand breaks by promoting local pairing of damaged and intact sister chromatids. In G2 phase, cohesin that is not bound to chromatin is inactivated, but its reactivation can occur in response to DNA damage. Recent papers by Koshland's and Sjögren's groups describe the critical role of the known cohesin cofactor Eco1 (Ctf7) and ATR checkpoint kinase in (...) damage‐induced reactivation of cohesin, revealing an intricate mechanism that regulates sister‐chromatid pairing to maintain genome integrity.1,2 BioEssays 30:5–9, 2008. © 2007 Wiley Periodicals, Inc. (shrink)

Many bacteria conditionally express proteinaceous organelles referred to here as microcompartments (Fig. 1). These microcompartments are thought to be involved in a least seven different metabolic processes and the number is growing. Microcompartments are very large and structurally sophisticated. They are usually about 100–150 nm in cross section and consist of 10,000–20,000 polypeptides of 10–20 types. Their unifying feature is a solid shell constructed from proteins having bacterial microcompartment (BMC) domains. In the examples that have been studied, the microcompartment shell (...) encases sequentially acting metabolic enzymes that catalyze a reaction sequence having a toxic or volatile intermediate product. It is thought that the shell of the microcompartment confines such intermediates, thereby enhancing metabolic efficiency and/or protecting cytoplasmic components. Mechanistically, however, this creates a paradox. How do microcompartments allow enzyme substrates, products and cofactors to pass while confining metabolic intermediates in the absence of a selectively permeable membrane? We suggest that the answer to this paradox may have broad implications with respect to our understanding of the fundamental properties of biological protein sheets including microcompartment shells, S‐layers and viral capsids. BioEssays 30:1084–1095, 2008. © 2008 Wiley Periodicals, Inc. (shrink)

In lieu of an abstract, here is a brief excerpt of the content:A PARABLE OF SCANDAL: SPECULATIONS ABOUT THE WHEAT AND THE TARES IN MATTHEW 13 John F. Cornell St. John's College, NM I will open my mouth in parables; I will utter things kept secret since the foundation of die world" (Matthew 13:35) The title ofone of René Girard's path-breaking books, Things Hidden since the Foundation ofthe World, is of course drawn from this passage. Few scholarly writings compare to (...) this discussion ofmimetic rivalry and collective persecution for conveying a sense of the Bible's depths and secrecy, and for deciphering the wisdom inscribed in its images. Yet it is curious that Girard eschews the parables said to be the repositories of scriptural secrets. Both in Things Hidden (189) and in Quand ces choses commenceront (171-172), he distances himself from the parables as conceding too much to the human desire for a mythical God ofretribution. In light of all we have learned from Girard about our traditional misreadings of the Gospel, we might well question even the strongest impressions left by some of its figures. Might the parables' language of apocalyptic vengeance conceal a different revelation, just as an innocent death on a cross should disclose not the bloodlust ofa deity but the violence ofmen? Matthew's Jesus tells a story about a sowerwhose crop was infested by his enemy's weeds, weeds to be uprooted and destroyed at the harvest. And, granted, when Jesus translates the parable for the disciples he describes divine violence at the end ofhistory. The enemy is the devil and the sower is the Son ofMan who sends out his angels to discern the unrighteous ofhumanity and cast them into the furnace. But something does not seem quite right. Isn't Jesus' reduction of his own parable a little too obvious? And is there not John F. Cornell99 something grotesque—or at least simple-minded—about the teacher who warned about dirowing pearls ofwisdom now turning around and broadcasting the solution to one of the great mysteries? If we look closely at the very parable that surrounds the suggestion of "things hidden since the foundation ofthe world," we may see, however, that something is indeed hidden, hidden in the text. And what is hidden there confirms Girard's insight about the dominion ofmimetic rivalry since the world began. The Wheat and Tares is more than amyth ofdivine punishment. It is a parable ofthe essential human malady, a parable ofscandal. Here is the main text of the parable of the Wheat and the Tares, from Matthew chapter 13, in the New King James Version.1 "The kingdom ofheaven is like a man who sowed good seed in his field (agroi) but while men slept, his enemy came and sowed tares among the wheat and went his way. But when the grainhad sprouted and produced a crop, then the tares also appeared. So the servants of the [master of the house] (oikodespotou) came and said to him, 'Sir, did you not sow good seed inyourfield? How then does it have tares?' He said to diem, 'An enemy, [a man,] has done this.' The servants said to him, 'Do you want us then to go and gather them up?' But he said, 1No, lest while you gather up the tares you also uproot the wheat with them. Let both grow together until the harvest, and at the time ofharvest I will say to the reapers, "First gather together the tares and bind them in bundles to bum them, but gather the wheat into my bam.'"" (Matthew 13: 24-30) Andhere is the explanation ofdie Wheat and die Tares that follows soon after: All these things Jesus spoke to the multitude in parables; and without a parable He did not speak to them, that it might be fulfilled which was spoken by the prophet saying: "1 will open my mouth in parables; I will utter things kept secret [since] the foundation ofthe world." 1 Minor corrections, necessary to reflect the Greek, are indicated by brackets. The source for the Greek text is the Nestle-Almond Novum Testamentum Graece. 100A Parable ofScandal Then Jesus sent the multitude away and went into the house (oikiari). And His... (shrink)

The cellular basis of motor learning in the cerebellum has been attributed mostly to long-term depression (LTD) at excitatory parallel fiber (PF)-Purkinje cell (PC) synapses. LTD is induced when PFs are activated in conjunction with a climbing fiber (CF), the other excitatory input to PCs. Recently, by using whole-cell patch-clamp recording from PCs in cerebellar slices, a new form of synaptic plasticity was discovered. Stimulation of excitatory CFs induced a long-lasting (usually longer than 30 min) of 30 sec) (...) and the durations of RP (>30 min) strongly suggests that some intracellular biochemical machinery is involved. Pharmacological evidence suggests that protein kinases are involved in RP of inhibitory synapses and LTD of excitatory PF synapses. Besides the well-described LTD, RP could be a cellular mechanism that plays an important role in motor learning. (shrink)

Despite the progress achieved over the last decade after the birth of the first cloned mammal, the efficiency of reproductive cloning remains invariably low. However, research aiming at the use of nuclear transfer for the production of patient‐tailored stem cells for cell/tissue therapy is progressing rapidly. Yet, reproductive cloning has many potential implications for animal breeding, transgenic research and the conservation of endangered species. In this article we suggest that the changes in the epi‐/genotype observed in cloned embryos arise from (...) unbalanced nuclear reprogramming between parental chromosomes. It is probable that the oocyte reprogramming machinery, devised for resident chromosomes, cannot target the paternal alleles of somatic cells. We, therefore, suggest that a reasonable approach to balance this asymmetry in nuclear reprogramming might involve the transient expression in donor cells of chromatin remodelling proteins, which are physiologically expressed during spermatogenesis, in order to induce a male‐specific chromatin organisation in the somatic cells before nuclear transfer. BioEssays 30:66–74, 2008. © 2007 Wiley Periodicals, Inc. (shrink)

Drosophila telomeres comprise DNA sequences that differ dramatically from those of other eukaryotes. Telomere functions, however, are similar to those found in telomerase‐based telomeres, even though the underlying mechanisms may differ. Drosophila telomeres use arrays of retrotransposons to maintain chromosome length, while nearly all other eukaryotes rely on telomerase‐generated short repeats. Regardless of the DNA sequence, several end‐binding proteins are evolutionarily conserved. Away from the end, the Drosophila telomeric and subtelomeric DNA sequences are complexed with unique combinations of proteins that (...) also modulate chromatin structure elsewhere in the genome. Maintaining and regulating the transcriptional activity of the telomeric retrotransposons in Drosophila requires specific chromatin structures and, while telomeric silencing spreads from the terminal repeats in yeast, the source of telomeric silencing in Drosophila is the subterminal arrays. However, the subterminal arrays in both species may be involved in telomere–telomere associations and/or communication. BioEssays 30:25–37, 2008. © 2007 Wiley Periodicals, Inc. (shrink)

Changes in the shape and structural organization of the cell nucleus occur during many fundamental processes including development, differentiation and aging. In many of these processes, the cell responds to physical forces by altering gene expression within the nucleus. How the nucleus itself senses and responds to such mechanical cues is not well understood. In addition to these external forces, epigenetic modifications of chromatin structure inside the nucleus could also alter its physical properties. To achieve a better understanding, we (...) need to elucidate the relationship between nuclear structure and material properties. Recently, new approaches have been developed to systematically investigate nuclear mechanical properties. These experiments provide important new insights into the disease mechanism of a growing class of tissue‐specific disorders termed ‘nuclear envelopathies’. Here we review our current understanding of what determines the shape and mechanical properties of the cell nucleus. BioEssays 30:226–236, 2008. © 2008 Wiley Periodicals, Inc. (shrink)

Genomes of higher eukaryotes contain abundant non‐coding repeated sequences whose overall biological impact is unclear. They comprise two categories. The first consists of retrotransposon‐derived elements. These are three major families of retroelements (LINEs, SINEs and LTRs). SINEs are clustered in gene‐rich regions and are found in promoters of genes while LINEs are concentrated in gene‐poor regions and are depleted from promoters. The second class consists of non‐coding tandem repeats (satellite DNAs and TTAGGG arrays), which are associated with mammalian centromeres, heterochromatin (...) and telomeres. Terminal TTAGGG arrays are involved in telomere capping and satellite DNAs are located in heterochromatin, which is implicated in transcription silencing by gene repositioning (relocalization). It is unknown whether interstitial TTAGGG sequences, which are present in many vertebrates, have a function. Here, evidence will be presented that retroelements and TTAGGG arrays are involved in regulation of gene expression. Retroelements can provide binding sites for transcription factors and protect promoter CpG islands from repressive chromatin modifications, and may be also involved in nuclear compartmentalization of transcriptionally active and inactive domains. Interstitial telomere‐like sequences can form dynamically maintained three‐dimensional nuclear networks of transcriptionally inactive domains, which may be involved in transcription silencing like classic heterochromatin. BioEssays 30:338–348, 2008. © 2008 Wiley Periodicals, Inc. (shrink)

BackgroundAntipsychotic-induced weight gain is a contributing factor in the reduced life expectancy reported amongst people with psychotic disorders. CYP2D6 is a liver enzyme involved in the metabolism of many commonly used antipsychotic medications. We investigated if CYP2D6 genetic variation influenced weight or BMI among people taking antipsychotic treatment.MethodsWe conducted a systematic review and a random effects meta-analysis of publications in Pubmed, Embase, PsychInfo, and CENTRAAL that had BMI and/or weight measurements of patients on long-term antipsychotics by their CYP2D6-defined metabolic groups.ResultsTwelve (...) studies were included in the systematic review. All cohort studies suggested that the presence of reduced-function or non-functional alleles for CYP2D6 was associated with greater antipsychotic-induced weight gain, whereas most cross-sectional studies did not find any significant associations. Seventeen studies were included in the meta-analysis with clinical data of 2,041 patients, including 93 poor metabolizers, 633 intermediate metabolizers, 1,272 normal metabolizers, and 30 UMs. Overall, we did not find associations in any of the comparisons made. The estimated pooled standardized differences for the following comparisons were PM versus NM; weight = –0.07, BMI = 0.40. IM versus NM; weight = 0.09 and BMI = 0.09. UM versus EM; weight = 0.01 and BMI = –0.08.ConclusionOur systematic review of cohort studies suggested that CYP2D6 poor metabolizers have higher BMI than normal metabolizers, but the data of cross-sectional studies and the meta-analysis did not show this association. Although our review and meta-analysis constitutes one of the largest studies with comprehensively genotyped samples, the literature is still limited by small numbers of participants with genetic variants resulting in poor or UMs status. We need further studies with larger numbers of extreme metabolizers to establish its clinical utility in antipsychotic treatment. CYP2D6 is a key gene for personalized prescribing in mental health. (shrink)

The centromere is the region of the chromosome where the kinetochore forms. Kinetochores are the attachment sites for spindle microtubules that separate duplicated chromosomes in mitosis and meiosis. Kinetochore formation depends on a special chromatin structure containing the histone H3 variant CENP‐A. The epigenetic mechanisms that maintain CENP‐A chromatin throughout the cell cycle have been studied extensively but little is known about the mechanism that targets CENP‐A to naked centromeric DNA templates. In a recent report published in Science,1 (...) such de novo centromere assembly of CENP‐A is shown to be dependent on heterochromatin and the RNA interference pathway. BioEssays 30:526–529, 2008. © 2008 Wiley Periodicals, Inc. (shrink)

Kadim bir dil olan Arapça, İslamiyet’in gelişiyle birlikte kutsal bir kitap olan Kur’an-ı Kerim’in dili olmuş, günümüze kadar varlığını ve önemini korumuştur. Anadili Arapça olmayanlara bu dilin öğretilmesi için farklı coğrafya ve farklı dönemlerde medreselerde veya devletin resmi eğitim kurumlarında Arapça dersleri verilmiştir. Verilen Arapça eğitimlerinde nahiv ve sarf temelli bir eğitim uygulanmıştır. Gramere dayalı bu dil eğitimi İlahiyat veya İslami İlimler fakültelerinde verilen eğitimlerle daha ileri bir seviyeye taşınmaya çalışılmıştır. İlahiyat veya İslami İlimler fakültelerinde verilen Arapça eğitimleri temelde dört (...) beceriyi kazandırma odaklı hazırlanmıştır. Ancak gramer odaklı verilmeye çalışılan Arapça eğitimlerinde hedeflenen başarı elde edilememektedir. Bu araştırmanın konusu, İlahiyat veya İslami İlimler fakültelerinde okutulan Arapça hazırlık eğitiminin değerlendirilmesi ve bu eğitimin kalitesinin arttırılması için yapılması gerekenlerdir. Bu doğrultuda bu çalışmada Bitlis Eren Üniversitesi İslami İlimler Fakültesi %30 Zorunlu Arapça Hazırlık Sınıflarının 2018-2019 Eğitim ve Öğretim Yılında aldıkları Arapça eğitimi model alınmıştır. Hazırlık Sınıflarında Arapça eğitimi alan 90 öğrenciye 33 sorudan oluşan anket uygulanmıştır. Anket sonuçları değerlendirilmiş ve verilen Arapça eğitiminin kalitesi ortaya konulmuştur. Elde edilen sonuçlardan hareketle, verilen eğitimde eksik ve hatalı durumlar tespit edilerek, eğitimin daha kaliteli bir düzeye ulaştırılması için önerilerde bulunulmuştur. Giriş bölümünde İlahiyat veya İslami İlimler fakültelerindeki Arapça eğitimi hakkında bilgiler verilmiştir. Sonrasında çalışmanın amacı, önemi, sınırlılıkları, problemi ve örneklem ortaya konulmuştur. Fakültede uygulanan Arapça hazırlık eğitimi hakkında bilgiler verilmiş ve uygulanan anket çalışmaya eklenmiştir. Son olarak bulgular ve yorumlar verilerek çalışma sonuç ve öneriler ile sonlandırılmıştır. (shrink)

This paper reports on action research that evaluated local perceptions and knowledge of indigenous tree planting and management in the Romwe catchment, Chivi District, southern Zimbabwe. The species tested were the overexploited Afzelia quanzensis, important for timber and carvings of sculptures and utensils; Sclerocarya birrea, the marula tree used for wood, bark, and fruit; and Brachystegia glaucescens, the dominant miombo tree species, used for firewood, fiber, and fodder. Participants volunteered to plant and manage the test seeds, while a research (...) team monitored their activities and results for 26 months. For Afzelia quanzensis, the germination rate was 81%, and 69% of the seedlings were still alive after one year. In the case of Sclerocarya birrea, the germination rate was 69%, and the one-year survival rate was 50%. For Brachystegia glaucescens, the germination rate was only 30%, and the survival rate was 31%. The main reasons for planting were to provide shade, to serve as a windbreak, and to conserve and gain individual control over dwindling natural resources, particularly Afzelia quanzensis. Women were generally more active and innovative than men. For instance, they searched for their own seeds or seedlings in the bush when there weren’t sufficient plants. Some participants tried out various indigenous methods of pest and disease control, water conservation, and moisture retention. Group feedback sessions and informal interactions provided the opportunity to share experiences. The participants learned that indigenous trees can be purposefully planted and were not simply a gift from God. Despite the droughts and political instability of recent years, a growing number of people became involved in tree planting during 2002–2003. As a result, there is now greater awareness among the local population of dwindling resources and their future potential. (shrink)

The present paper proposes a definition for the complex polysemic concepts of consciousness and awareness (in humans as well as in other species), and puts forward the idea of a progressive ontological development of consciousness from a state of ‘childhood’ awareness, in order to explain that humans are not only able to manipulate objects, but also their mental representations. The paper builds on the idea of qualia intended as entities posing regular invariant requests to neural processes, trough the permanence of (...) different properties. The concept of semantic differential introduces the properties of metaphorical qualia as an exclusively human ability. Furthermore this paper proposes a classification of qualia, according to the models–with different levels of abstraction–they are implied in, in a taxonomic perspective. This, in turn, becomes a source of categorization of divergent representations, sign systems, and forms of intentionality, relying always on biological criteria. New emerging image-of-the-world-devices are proposed, whose qualia are likely to be only accessible to humans: emotional qualia, where emotion accounts for the invariant and dominant property; and the qualic self where continuity, combined with the oneness of the self, accounts for the invariant and dominant property. The concept of congruence between different domains in a metaphor introduces the possibility of a general evaluation of truth and falsity of all kinds of metaphorical constructs, while the work of Matte Blanco enables us to classify conscious versus unconscious metaphors, both in individuals and in social organizations. (shrink)

Recently, Dewar (2019) has suggested that one can apply the strategy of 'sophistication' - as exemplified by sophisticated substantivalism as a response to the diffeomorphism invariance of General Relativity - to gauge theories such as electrodynamics. This requires a shift to the formalism of fibre bundles. In this paper, I develop and defend this suggestion. Where my approach differs from previous discussions is that I focus on the metaphysical picture underlying the fibre bundle formalism. In particular, I aim to affirm (...) the physical reality of gauge properties. I argue that this allows for a local and separable explanation of the Aharonov-Bohm effect. Its puzzling features are explained by a form of holism inherent to fibre bundles. (shrink)

In a long-term transformation, that begins in Antiquity but takes a crucial turn in the Renaissance anatomies, the “fibre” becomes from around 1750 the operative building block and at the same time the first unifying principle of function-structure-complexes of organic bodies. It occupies the role that the cell takes up in the cell œconomies of the second third of the nineteenth century. In this paper, I will first discuss some key notions, technical analogies, and images that are related to “fibre”-concepts (...) from Andreas Vesalius to Albrecht von Haller and then focus on Charles Bonnet's and Denis Diderot's fibre œconomies. In Bonnet's and Diderot's fibre œconomies, the self-active, regulating properties of fibre-agents and their material structures, that reach from fibre bundles, tissues and membranes to apparati of organs, are united within the concrete whole of individual organized “systems” or “networks.”. (shrink)

Although Wittgenstein is famously skeptical about the possibility of making substantial philosophical claims, he can be said to offer significant insights into the difference between inner and outer as well as the difference between self and other.1 He consistently reminds us that inner and outer are intimately connected instead of only causally related, as well as that the self—far from being a wholly independent entity—always already finds itself constituted by its relationships with others. In thus contesting the Cartesian view on (...) subjectivity, however, Wittgenstein may appear to simply reduce the inner to the outer and the self to the other.. (shrink)

:This article examines material economies of carbon fibre as a prosthetic form of masculinity. The paper advances three main arguments. Firstly, carbon fibre can be a site in which disability is overcome, an act of overcoming that is affected through masculinized technology. Secondly, carbon fibre can be a homosocial surface; that is, carbon fibre becomes both a surface extension of the self and a third-party mediator in homosocial relationships, a surface that facilitates intimacy between men in ways that devalue femininity (...) in both male and female bodies. Carbon fibre surfaces are material extensions of subjectivity, and carbon fibre surfaces are vectors of the cultural economies of masculine competition. Thirdly, the article gives an account of Oscar Pistorius as an example of the masculinization of carbon fibre, and the associated binding of a psychic attitude of misogyny and power to a form of violent and competitive masculine subjectivity. The paper unpacks the affects, economies and surfaces of “carbon fibre masculinity” and discusses Pistorius’ use of carbon fibre, homosociality and misogyny as forms of protest masculinity through which he unconsciously attempted to recuperate his gendered identity from emasculating discourses of disability. (shrink)

Women played a vital role in the American and British antislavery movements of the nineteenth century. Among other strategies, American women’s efforts included boycotting slave-produced goods and selling luxury items to raise money for the cause. Complicated by the nation’s diverse religious landscape, popular attitudes toward dress rendered some forms of consumer advocacy more effective than others. Fashionable antislavery fairs provided significant financial support for political campaigns. Meanwhile, Quaker Christians and some evangelical groups, which valued plain dress, promoted abstention from (...) all slave- produced goods and the genesis of an alternative free-labour cotton trade. Unlike the famous British boycotts of slave- produced sugar, American textile boycotts gained little support. This paper examines how the tension between American fashion and antifashion influenced the rise and fall of the free cotton movement. (shrink)

Women played a vital role in the American and British antislavery movements of the nineteenth century. Among other strategies, American women’s efforts included boycotting slave-produced goods and selling luxury items to raise money for the cause. Complicated by the nation’s diverse religious landscape, popular attitudes toward dress rendered some forms of consumer advocacy more effective than others. Fashionable antislavery fairs provided significant financial support for political campaigns. Meanwhile, Quaker Christians and some evangelical groups, which valued plain dress, promoted abstention from (...) all slave- produced goods and the genesis of an alternative free-labour cotton trade. Unlike the famous British boycotts of slave- produced sugar, American textile boycotts gained little support. This paper examines how the tension between American fashion and antifashion influenced the rise and fall of the free cotton movement. (shrink)

During the 1840s and 1850s the British embryologist and histologist Martin Barry (1802-1855) propounded a bold and original thesis about the microscopic structure of animal and vegetable tissue. He maintained that minute double spirals were virtually ubiquitous in the makeup of a wide range of structures. This paper considers how a claim of this kind was consonant with a romantic image of scientific creativity with which Barry identified. It describes his partially successful strategies to convince contemporaries of the veracity of (...) his claims. Major figures in the field, such as Richard Owen and Jan Evangelista Purkyně, affirmed that Barry's spirals were real objects in nature. Others, notably William Sharpey, became convinced that the spirals were mere artefacts and that Barry was deeply flawed as a scientific investigator. The ultimate rejection of his hypothesis had much to do with the moral repugnance that Barry's attempts to gain credit for a major discovery evoked among influential medical scientists. This negative assessment of Barry as an investigator reveals the lineaments of an alternative ethic of scientific practice. (shrink)

Fibre suspension has garnered considerable attention in turbulent flows that are used in many industries. Solid particles, such as dust particles, notably affect the turbulent flow field in a rotational frame. In assessing their impacts, the dusty turbulent flow for fibre suspensions needs to be studied in a frame of rotation that can be substantially applied in many industries. This study, therefore, aims to build a theoretical model for the energy motion of dusty turbulent flow of fibre suspensions in a (...) rotational frame. The turbulence momentum equation was considered to formulate the model in presence of dusty fluid rotating flow of fibre suspensions. The newly derived equation was derived in second-order correlation tensors Fi,j, Wi,j, Gi,j, Si,j, Xi,j, Yi,j, Qi,j, and Ri,j at any two points in the flow domain, where the tensors were expressed as space, time, and distance functions. The developed model is a considerable improvement because it takes into account all of the potential influential parameters that could affect the motion of turbulent energy, such as dust particles, suspending particles, and rotating frame. However, the impact of these parameters on turbulence energy motion must be evaluated in order to assess the performance of turbulence systems utilized in a variety of industries, such as paper manufacturing. The present theoretical development will contribute to open up experimental and numerical research opportunities for the advancement of the industry, science, and technology. (shrink)

L’ouvrage d’A. Wenger est la version remaniée de sa thèse de doctorat complétée par certains développements parus dans des revues spécialisées, Clio. Histoire, Femmes et Sociétés, Dix-huitième Siècle… La reprise de travaux antérieurs a entraîné parfois quelques redondances – sur l’onanisme ou la lectrice, par exemple – qu’une lecture plus attentive aurait pu éviter. Cette restriction est mineure au regard de l’intérêt de cette étude dont « l’objectif est de faire bouger les objets d’analyse t...

1. The current political scene in many countries is populated by polarised groups with sharply contrasting loyalties and beliefs implying that there are fundamental schisms between opposing groups....

In this essay, I propose that DNA‐binding anti‐cancer drugs work more via chromatin disruption than DNA damage. Success of long‐awaited drugs targeting cancer‐specific drivers is limited by the heterogeneity of tumors. Therefore, chemotherapy acting via universal targets (e.g., DNA) is still the mainstream treatment for cancer. Nevertheless, the problem with targeting DNA is insufficient efficacy due to high toxicity. I propose that this problem stems from the presumption that DNA damage is critical for the anti‐cancer activity of these drugs. (...) DNA in cells exists as chromatin, and many DNA‐targeting drugs alter chromatin structure by destabilizing nucleosomes and inducing histone eviction from chromatin. This effect has been largely ignored because DNA damage is seen as the major reason for anti‐cancer activity. I discuss how DNA‐binding molecules destabilize chromatin, why this effect is more toxic to tumoral than normal cells, and why cells die as a result of chromatin destabilization. (shrink)