Since their derivation, human embryonic stem (hES) cells have been used for a variety of applications including developmental biology, pathology, chemical biology, genomics, and proteomics. However, their most important potential application is the generation of cells and tissues, which can be used for cell‐based therapies. One of the main drawbacks of hES cell culture is that they are particularly sensitive to dissociation, which is required for passaging, expansion, cryopreservation, and other applications. Recently, it has been discovered that an inhibitor of (...) Rho kinase (ROCKi; Y‐27632) increases the survival rate of dissociated, single hES cells. This breakthrough has allowed new methods in hES cell culture to be developed, with the promise of increasing hES cell numbers into the realm of clinical relevance. In our studies demonstrating that ROCKi dramatically increases hES cell cryopreservation efficiency, we have observed that ROCKi treatment does not decrease hES cell's susceptibility to apoptosis. Rather, we hypothesize that ROCKi treatment desensitizes single hES cells to their environment reducing the odds that individual cells will undergo anoikis. (shrink)

Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) can self‐renew indefinitely and contribute to all tissue types of the adult organism. Stem cell‐based therapeutic approaches hold enormous promise for the cure of regenerative diseases. Over the last few years, several studies have attempted to decipher the important role of transcription factor networks and epigenetic regulatory signals in the maintenance of ESC pluripotency, but the exact underlying mechanisms have yet to be identified. Among the epigenetic factors, chromatin dynamics and (...) structure have been found to contribute greatly to maintenance of pluripotency and regulation of differentiation in ESCs. These modifications include: covalent histone acetylation and methylation, histone bivalents and chromatin remodeling, and DNA methylation. Studies in ESCs have shown that genes associated with early development are arranged within a bivalent chromatin structure. This is thought to be a “poised yet repressed” situation, which can be activated upon differentiation. The breakthrough of iPSCs has opened a new era in stem cell biology. During reprogramming, the chromatin state of differentiated cells is reset to an embryonic form via a largely unknown mechanism. In this review, the fundamental impact of chromatin dynamic in ESCs as well as its critical role in the generation of iPSCs is discussed. (shrink)

Embryonic stem cells (ESCs) are now classified into two types of pluripotency: “naïve” and “primed” based upon their differing characteristics. Conventional human ESCs have much more in common with mouse epiblast stem cells and are now deemed to be primed. Naïve human ESCs that resemble mouse ESCs have recently been generated from their primed counterpart by cellular reprogramming. Isolation of naïve hESCs from human embryos has proven to be difficult. Is the inability to capture naïve hESCs the result of suboptimal (...) derivation conditions or because they are so transient they cannot be “captured” in vitro? Prevailing evidence surrounding this issue are inconclusive and require additional human embryo research. However, negative public opinion regarding human embryo research, may make this an uphill battle. The solution may come from cellular reprogramming. (shrink)

In the Caribbean as in many other areas costly biomedical resources and personnel are limited, and more and more people are turning to alternative medicine and folk practitioners for health care. To meet the goal of providing health care for all, research on nonbiomedical therapies is needed, along with legal recognition of folk practitioners to establish standards of practice.

This paper examines the consequences of a culture of “personal ethics” when using new methodologies, such as the use of social media sites as a source of data for research. Using SM research as an example, this paper explores the practices of a number of actors and researchers within the “Ethics Ecosystem” which as a network governs ethically responsible research behaviour. In the case of SM research, the ethical use of this data is currently in dispute, as even though it (...) is seemingly publically available, concerns relating to privacy, vulnerability, potential harm and consent blur the lines of responsible ethical research behaviour. The findings point to the dominance of a personal, bottom-up, researcher-led, ‘ethical barometer’ for making decisions regarding the permissibility of using SM data. We show that the use of different barometers by different researchers can lead to wide disparities in ethical practice - disparities which are compounded by the lack of firm guidelines for responsible practice of SM research. This has widespread consequences on the development of shared norms and understandings at all levels, and by all actors within the Ethics Ecosystem, and risks inconsistencies in their approaches to ethical decision-making. This paper argues that this governance of ethical behaviour by individual researchers perpetuates a negative cycle of academic practice that is dependent on subjective judgements by researchers themselves, rather than governed by more formalised academic institutions such as the research ethics committee and funding council guidelines. (shrink)

Realising the societal gains from publicly funded health and medical research requires a model for a reflexive evaluation precedent for the societal impact of research. This research explores UK Research Excellence Framework evaluators’ values and opinions and assessing societal impact, prior to the assessment taking place. Specifically, we discuss the characteristics of two different impact assessment extremes – the “quality-focused” evaluation and “societal impact-focused” evaluation. We show the wide range of evaluator views about impact, and that these views could be (...) conceptually reflected in a range of different positions along a conceptual evaluation scale. We describe the characteristics of these extremes in detail, and discuss the different beliefs evaluators had which could influence where they positioned themselves along the scale. These decisions, we argue, when considered together, form a dominant definition of societal impact that influences the direction of its evaluation by the panel. (shrink)

If A confirms H and B confirms H, it seems reasonable to infer that A&B confirms H. However, this inference is not valid; it is only a heuristic. I show that the level of confirmation A and B each give to H by itself implies nothing about the level of confirmation that A&B gives to H. Any combination of values is possible for P(H), P(H|A), P(H|B) and P(H|AB) is possible. Still, I show the heuristic leads from true premises to true (...) conclusions whenever A and B are statistically independent or correlated,which includes most situations where we evaluate evidence. There is risk, though, when one does not consider how A and B interact; one can miss anti-correlations that lead the heuristic to fail. The problem is exacerbated when groups of people are motivated to support a favored hypothesis. Each person finds evidence that confirms the hypothesis and contributes that evidence to the group’s discourse (e.g. through a climate change denial website). The temptation to focus solely on the number of individual pieces of evidence can lead people to ignore whether the evidence fits together. In combination with cognitive biases, this can lead to serious errors. (shrink)

The recent pop culture iconography of the Critical Race Theory (CRT) label has attracted more devoted (white) fans than a 90s boy band. In philosophy, this trend is evidenced by the growing number of white feminists who extend their work in gender analogically to questions of race and identity. The trend is further evidenced by the unchecked use of the CRT label to describe (1) any work dealing with postcolonial authors like W.E.B. Du Bois and Frantz Fanon or (2) the (...) role postcolonial themes like power, discourse, and the unconscious play in the social constructionist era. While this misnomer may seem practically insignificant, the artifice formerly known as CRT in philosophy—more adequately labeled “critical theories of race”— has been axiomatically driven by the political ideals of integration and by a revisionist commentary that seeks to expand traditional philosophical ideas, such as reason, history, and humanity, which were previously closed off by racial borders, to people of color. This “revision in the name of inclusion,” however, is not without its consequences. In order to incorporate the experiences of those who suffer under the weight of modernity and are marred by the burdens of racism into the narration of Continental and American philosophy, the theoretical perspectives in Critical Race Theory that deny the legitimacy of philosophy’s diversity agenda must necessarily be excluded.In particular, this recent move to recognize the study of race as a category of philosophical relevance has resulted in the outright denial of the nationalist and revolutionary fervor contained in the intellectual history specific to the Critical Race Theory movement started by the works of Derrick Bell.2 Instead, this new movement favors narratives that inculcate the ideals of a post-racial humanity and racial amelioration between compassionate (Black and white) philosophical thinkers dedicated to solving America’s race problem. (shrink)

In lieu of an abstract, here is a brief excerpt of the content:TOWARD A RELIGIOUS ETHICS OF TECHNOLOGY: A REVIEW DISCUSSION [I]t seems to me that Schema 18 [preparatory draft for the Pastoral Constitution on the Church in the Modern World] needs to rest on a deeper realization of the urgent problems posed by technology.... (The Constitution on Mass Media seems to have been totally innocent of any such awareness.) For one thing, the whole massive complex of technology, which reaches (...) into every aspect of social life today, implies a huge organization of which no one is really in control, and which dictates its own solutions irrespective of human needs or even reason.... I am not of course saying that technology is "bad," and that progress is something to be feared. But I am saying that behind the cloak of specious myths about technology and progress, there seems to be at work a vast uncontrolled power which is leading man where he does not want to go... and in which the Church... ought to be somewhat more aware of the intervention of the "principalities and powers" of which St. Paul speaks. -Thomas Merton, Letter to Bernard Haring (December ~6, 1964) l WHEN A DRAFT of the United States Roman Catholic bishops' proposed pastoral letter on human values was circulated to philos·ophy teachers at Catholic colleges in 1975, one respondent took the occasion to suggest that what was needed was not another geneml restatement of " human values " or a piecemeal analysis of specific issues (unemployment, artificial contraception, abortion, nuclear weapons, etc.) but something intermediate-work toward the development of an ethics of technology.2 Unbeknown i The Hidden Ground of Love: The Letters of Thomas Merton on Religious Experience and Social Concerns, selected and edited by William H. Shannon (New York: Farrar, Straus, Giroux, 1985), pp. 383-384. 2 The pastoral subsequently appeared as To Live in Christ Jesus: A Pastoral Reflection on the Moral Life (Washington, DC: U.S. Catholic Conference, November 1976). 146 TOWARD A RELIGIOUS ETHICS OF TECHNOLOGY 147 to himself, he had voiced a concern similar to that expressed by Thomas Merton a decade earlier. With the publication another decade later of Technological Powers and the Person: Nuclear Energy and Reproductive Technology,3 the proceedings of a workshop for Catholic bishops by the Pope John XXIII Medical-Moral Research and Education Center (then in St. Louis, now in Boston) there is evidence that this need is being increasingly recognized. Working from cmss-disciplinary analyses of two commonly un-associaited issues-nuclear energy and reproductive technologies (i.e., the ostensibly positive correlates 0£ nuclear weapons and artificial contraception)-Technological Powers and the Person seeks to formulaite general guidelines for the engagement with modern technology. Two specific technologies are rightly seen as related aspects of a global phenomenon, and philosophical anthropology is properly proposed as the foundation for ethical principles. Although aspects of this assessment may well be criticized, the general approach-a.s well as this particular workshop and the form of its final proceedingsdeserve commendation. 'Vhat follows, then, are some comments on format and substantive content aimed at furthering such a project. 1. The workshop itself (which was held in Dallas, Texas) was opened on the evening of January 31, 1983, with a keynote address by Christopher Derrick, a Catholic layman from England who has written on moral and religious issues of cuLture s Albert S. Moraczewski, O.P., Donald G. McCarthy, Edward J. Bayer, S.T.D., Michael P. McDonough, S.T.D., and Larry D. Lossing, eds., Technological Powers and the Person: Nuclear Energy and Reproductive Technologies (St. Louis, MO: Pope John Center, 1983). Pp. xiii, 500. This is the third in a series. Previous proceedings are New Technologies of Birth and Death (196 pages, from a 1980 workshop) and Human Sexuality and Personhood (254 pages, from a 1981 workshop), both published by the Pope John Center; as the titles and pages alone indicate, neither has the scope of the present volume. 148 CARL MITCHAl\1 and eoology.4 Together with a general overview of the workshop by the senior editor, Derrick's address constitutes part ooo of the proceedings. His fundamental question concerns whether there might not be an "inordinate attachment to temporal good... (shrink)

The last phase of Greek philosophy has until recently been less intelligently studied than any other, and in our understanding of its development there are still lamentable lacunae. Three errors in particular have in the past prevented a proper appreciation of Plotinus' place in the history of philosophy. The first was the failure to distinguish Neoplatonism from Platonism: this vitiates the work of many early exponents from Ficinus down to Kirchner. The second was the belief that the Neoplatonists, being ‘mystics,’ (...) were necessarily incomprehensible to the plain man or even to the plain philosopher. To have encouraged the persistence of this superstition in the nineteenth century is the least pardonable of Creuzer's many sins. The third was the chronological confusion involved in the ascription to Saint Paul's contemporary of the works of the pretended Dionysius Areopagita, which contain a fully-developed Neoplatonic theology. Though the fraud had been exposed by Scaliger, these writings continued down to the beginning of the nineteenth century to be used as evidence that the ‘Nsoplatonic trinity’ was an inferior imitation of the Christian one. When this false trail was at length abandoned the fashion for orientalizing explanations persisted in another guise: to the earliest historians of Neoplatonism, Simon and Vacherot, the school of Plotinus was ‘the school of Alexandria,’ and its inspiration was mainly Egyptian. Vacherot says of Neoplatonism that it is ‘essentially and radically oriental, having nothing of Greek thought but its language and procedure.’ Few would be found to-day to subscribe to so sweeping a pronouncement; but the existence of an important oriental element in Plotinus' thought is still affirmed by many French and German writers. (shrink)

C.F Gauss’s computational work in number theory attracted renewed interest in the twentieth century due to, on the one hand, the edition of Gauss’s Werke, and, on the other hand, the birth of the digital electronic computer. The involvement of the U.S. American mathematicians Derrick Henry Lehmer and Daniel Shanks with Gauss’s work is analysed, especially their continuation of work on topics as arccotangents, factors of n2 + a2, composition of binary quadratic forms. In general, this strand in Gauss’s (...) reception is part of a more general phenomenon, i.e. the influence of the computer on mathematics and one of its effects, the reappraisal of mathematical exploration. (shrink)

Thomas E. Hill, Jr., interprets and extends Kant's moral theory in a series of essays that highlight its relevance to contemporary ethics. He introduces the major themes of Kantian ethics and explores its practical application to questions about revolution, prison reform, and forcible interventions in other countries for humanitarian purposes.

This line has been thought corrupt by most editors, though there is no agreement on the remedy. The Herald is plainly asking why the people at home are despondent: picks up the Chorus's phrase . But as Wilamowitz says, ‘ de populo aut senatu Argivorum accipi non potest’: it can only mean the army at Troy, as in lines 538 and 545. The usual inference is that arparw is corrupt.

Keine ausführliche Beschreibung für "Organon der gesammten transcendenten Analysis, Teil 1: Transcendente Elementarlehre" verfügbar.

The purpose of this article is to elaborate the many literary allusions that Simone Weil used in her ultimate work: L' Enracinement, translated as The Need for Roots, to achieve her goal of encouraging her fellow countrymen to create a new postwar society. Understanding how she used the riches of the French and Western Literary Cannon, less easily grasped by those not educated in the French Education system, enriches the understanding of Weil's purpose and skill in writing on many levels, (...) simultaneously for different target audiences. Underlying her stress on the need for truth and honesty about a county's past and present, with discernable respect for every person, is her foundational belief in the spiritual destiny of every human being. Examining her literary allusions in detail to show her clever subversion of traditionally accepted interpretations brings a new dimension to Simone Weil studies, while underlining the relevance of this essay to contemporary dilemmas. (shrink)

the aidos of phaedra and the meaning of the hyppolytus.

The role of imagination in Descartes. The critic of Sartre to the concept of image in Descartes.

The attempts of Verrall and Tucker to discover, without resorting to emendation, a construction for the participles in v. 285 have convinced only their authors. All other recent scholars either postulate a lacuna before this line or transpose it to follow v. 288, if they do not delete it altogether. All alike assume that both participles describe the behaviour of the victim of the underworld powers.

As the later phases of Greek thought are at last beginning to receive in England and Ireland the attention they merit, it is hoped that the following critical notes on Plotinus may be of interest to a few readers. Some involve points of doctrine; others are intended to illustrate certain shortcomings of the German school, who have hitherto been practically the sole workers in the textual field.

Mr. G. S. Kirk's interesting and illuminating treatment of this curious fragment in CQ,. xliv , pp. 149–57, prompts me to offer some further conjectures on the reading and interpretation of lines 15–23, on the character of the fragment as a whole. I am indebted for valuable help both to Mr. Kirk himself and to Mr. C. H. Roberts.

This study juxtaposes Simone Weil's exposition of God's invitation to know and love the good through the divine signature of beauty stamped on the order of the world and Flannery O'Connor's depiction of a society whose oppressive order allows some characters to oppose outright a divine order or to live under the illusion that the divine invitation is irrelevant because they, in their egoism and materialist values, are the centre of the universe. An examination of O'Connor's and Weil's ideas on (...) order and beauty, grace and decreation, within the disorders of their contexts, reveals both writers' skills in pointing prophetically through disorder to divine order, thus, disclosing bridges of revelation. (shrink)

Insight experiences are sudden, persuasive, and can accompany valuable new ideas in science and art. In this preregistered experiment, we aim to validate a novel visceral and continuous measure of insight problem solving and to test whether real-time and embodied feelings of insight can predict correct solutions. We report several findings. Consistent with recent work, we find a strong positive relationship between Aha moments and accuracy for problems that demand implicit processing. We also found that the intensity of the insight (...) experience further predicted the accuracy of solutions and participants naturally embodied the intensity of their insight experiences by squeezing the dynamometer more tightly. Intriguingly, this unintentional embodiment further predicted the accuracy of solutions. We suggest that the dynamometer complements previous measures by (1) simultaneously capturing both process and feeling in real-time, (2) highlights the value of measuring Aha moments on a continuum of intensity, and (3) firmly establishes that the impulsive feeling of Aha can carry information about the veracity of an idea. We discuss the findings in light of a recent theoretical account of how feelings of insight may act as a heuristic to select ideas from the stream of consciousness. (shrink)

In light of the development of “CRISPR” technology, new promising advances in therapeutic and preventive approaches have become a reality. However, with it came many ethical challenges. The most recent worldwide condemnation of the first use of CRISPR to genetically modify a human embryo is the latest example of ethically questionable use of this new and emerging field. Monotheistic religions are very conservative about such changes to the human genome and can be considered an interference with God’s creation. Moreover, these (...) changes could cause perpetual changes to future generations. The Muslim scholars establish their decisions by addressing five foundations of Islamic law i.e. “maqāṣid al sharı̄`a”; the purposes of the law. These are dın̄, nafs, nasl, `aql and māl. To achieve this, the five principles should all be met before approval of an experiment like the Chinese embryo modifications; Qaṣd which is achieved in this case as it aims to protect the embryo from HIV. Yaqın̄ and Ḍarar were not satisfied as they require strong scientific certainty of the procedures, and evidence of safety. Ḍarūra by which the alternatives being compared; in this case more established and proven safe alternatives to protect the HIV transmission from the father are available, so this principle is not met. The final principle is `Urf, by which the social context of using any contemporary technology should be taken in consideration, and clearly this was not achieved. Collectively, germline changes are rejected from an Islamic perspective until the five principles are fulfilled. In the Chinese Twins gene editing case, there was clearly no justification or support for it according to the Muslim Jurisprudence laws. These laws and approaches can serve as an ethical checklist for such controversial research, especially in early stages of the research. (shrink)

E. J. Lowe; Coinciding objects: in defence of the ‘standard account’, Analysis, Volume 55, Issue 3, 1 July 1995, Pages 171–178, https://doi.org/10.1093/analys/5.