Panenmentalist possibilities are individual pure possibilities existing independently of any mind, actual reality, and possible-world conception. These possibilities are a priori accessible to our intellect and imagination. In this paper, I attempt to shed some panenmentalist light on the discovery of chemical branched chain reactions and its implications on biology and cancer research. I also examine the case of the Belousov–Zhabotinsky reaction which, at first, was believed to be impossible. Finally, I proceed to examine through a panenmentalist lens (...) Szilard’s discovery of the possibility of physical chain reactions and Mullis’s discovery of a polymerase chain reaction. All these major actual discoveries clearly depend on the discovery of the relevant panenmentalist possibilities and the ways in which they relate to one another. (shrink)

A history of the Atomic Bomb from Marie Curie to Hiroshima. “I am become Death, the destroyer of worlds” — Oppenheimer quoting the Bhagavad Gita after witnessing the successful demonstration of the atom bomb. The bomb, which killed an estimated 140,000 civilians in Hiroshima and destroyed the countryside for miles around, was one of the defining moments in world history. That mushroom cloud cast a terrifying shadow over the contemporary world and continues to do so today. But how could this (...) have happened? What led to the creation of such a weapon of mass destruction? From the moment scientists contemplated the destructive potential of splitting the atom, the role of science changed. Ethical and moral dilemmas faced all those who realized the implications of their research. Before the Fall-Out charts the chain of events from Marie Curie’s scientific breakthrough through the many colourful characters such as Einstein, Robert Oppenheimer and Lord Rutherford, whose discoveries contributed to the bomb. The story of the atomic bomb spans 50 years of prolific scientific innovation, turbulent politics, foreign affairs and world-changing history. Through personal stories of exile, indecision and soul-searching, to charges of collaboration, spying and deceit, Diana Preston presents the human side of an unstoppable programme with a lethal outcome. (shrink)

We consider a chain of metabolic reactions catalyzed by enzymes, of reversible Michaelis-Menten type with full dynamics, i.e. not reduced with any quasi-steady state approximations. We study the corresponding dynamical system and show its global stability if the equilibrium exists. If the system is open, the equilibrium may not exist. The main tool is monotone systems theory. Finally we study the implications of these results for the study of coupled genetic-metabolic systems.

Nitric oxide (NO) is involved in synaptic long-term potentiation (LTP) by multiple signaling pathways. Here, we show that LTP of synaptic transmission can be explained as a feature of signal transduction—bistable behavior in a chain of biochemical reactions with positive feedback, formed by diffusion of NO to the presynaptic site and facilitating the release of glutamate (Glu). The dynamics of Glu, calcium (Ca2+) and NO is described by a system of nonlinear reaction–diffusion equations with modified Michaelis–Menten (MM) kinetics. (...) Numerical investigation reveals that the chain of biochemical reactions analyzed can exhibit a bistable behavior under physiological conditions when production of Glu is described by MM kinetics and decay of NO is modeled by means of two enzymatic pathways with different kinetic properties. Our finding extends understanding of the role of NO in LTP: a short high-intensity stimulus is “memorized” as a long-lasting elevation of NO concentration. The conclusions obtained by analysis of the chain of biochemical reactions describing LTP can be generalized to other chains of interactions or for creating the logical elements for biological computers. (shrink)

In the metabolic control theory, the control coefficient is a key parameter in quantifying the sensitivity of the flux towards an infinitesimal variation of enzyme activity. This concept does not apply just as it is for variations of enzyme concentrations whenever there is spatial, energy or resources limitations in the cell. Due to constraint on total enzyme concentration, the variation of concentration of any given enzyme may affect the concentrations of other enzymes. To take into account these correlations between enzyme (...) concentrations, we propose the concept of "combined response coefficient". Its definition is similar to that of the control coefficient, but its mathematical expression is different. Its range of variation is from – + 1, the null value corresponding to optimum enzyme concentration, i.e. to concentrations that maximise the flux, and the negative values to concentrations beyond the optimum value. A summation property could be derived using a simple weighting of the combined response coefficients, the sum of the weighed coefficient being 0. (shrink)

In this study, we examine investor and firm response to the California Transparency in Supply Chains Act of 2010. The CTSCA requires large retail and manufacturing firms to disclose efforts to eradicate slavery and human trafficking from their supply chains and is a rare example of mandated corporate social responsibility disclosure. Based on a sample of 105 retail companies subject to the CTSCA, we find a significant negative market reaction to the passing of the CTSCA. Furthermore, we find that (...) the reaction is significantly more negative for larger firms and companies facing greater supply chain risks, suggesting that investors place a negative value on exposure to legitimacy threats in the social domain. With respect to company disclosure response, we document relatively high compliance with the legislation, although we also find that the disclosure response appeared to be more symbolic than substantive in nature. Finally, our analysis indicates that both disclosure choice and disclosure extensiveness were significantly higher for the high-supply chain risk companies, suggesting that the response was influenced by concerns with strategic legitimation. Overall, the limited quality of disclosure suggests that, without additional rules and guidance, mandates alone may not lead to meaningful social disclosure. (shrink)

Il est essentiel pour une chaîne de télévision, qu'elle soit publique ou privée, de connaître son public. Le groupe Francetélévisions s'est doté d'une direction d'études commune et de trois directions affiliées à chaque chaîne pour regrouper et analyser l'ensemble des données disponibles sur l'auditoire de la télévision. Face à la variété des outils et à la profusion des données, il est primordial de bien sélectionner ce qui est le plus pertinent pour répondre aux demandes des responsables de programme et de (...) programmation. La direction des études décortique les résultats d'audience par cible et utilise les logiciels permettant de suivre les flux de publics, les reports d'une chaîne à l'autre, etc. Les études qualitatives sont indispensables pour anticiper les réactions du public, pour améliorer un programme. Des sondages proposés par des instituts spécialisés complètent ces études internes .It is essential for both public and private television channels to know their audience. The France Télévisions group has set up a central research department covering each of its channels which also have their own individual research departments whose aim is to assemble and analyse all available audience data. Given the variety of tools and the large amount of data available, it is imperative to select well that which is the most relevant to answer demands coming from programme planners. The research and study department analyses audience ratings per target and uses software to follow audience movement, changes from one channel to another and so forth. Qualitative studies are essential to foresee audience reactions and to improve a program. Surveys proposed by specialised institutes complete these internal studies. (shrink)

We consider metabolic networks with reversible enzymatic reactions. The model is written as a system of ordinary differential equations, possibly with inputs and outputs. We prove the global stability of the equilibrium , using techniques of monotone systems and compartmental matrices. We show that the equilibrium does not always exist. Finally, we consider a metabolic system coupled with a genetic network, and we study the dependence of the metabolic equilibrium with respect to concentrations of enzymes. We give some conclusions concerning (...) the dynamical behavior of coupled genetic/metabolic systems. (shrink)

La profesión de carpintero de ribera, sus técnicas, las herramientas utilizadas, los productos finales: los barcos, así como los conocimientos necesarios para su fabricación forman parte del patrimonio cultural marítimo universal. La construcción en madera es un oficio milenario que está en peligro de desaparición, y con ella uno de los elementos identitarios más importantes de las regiones costeras europeas. La escasa historiografía académica sobre los carpinteros de ribera como protagonistas es el principal motivo de este trabajo, que se adentra (...) en sus raíces, dando a conocer algunos de los aspectos más relevantes que definen e identifican la importancia del tema, como las principales actividades desarrolladas, su contextualización histórica, las técnicas, herramientas y la lengua franca marinera que compartían. Por ello, el fin último es proporcionar una fuente de referencia a quiénes apuesten, tanto de manera teórica como práctica, por la recuperación y la conservación de esta profesión ancestral sobre la que apenas existe información e investigación. Se concluye que la recuperación y gestión de este patrimonio, con vinculaciones amplias con otras actividades relacionadas, puede abrir perspectivas de consolidación patrimonial y económica en espacios marítimos poco desarrollados, ya que puede servir para sentar las bases de un futuro turismo cultural en el que estas técnicas sean protagonistas y sirvan para potenciar la identidad marítima en las regiones litorales. (shrink)

This chapter focuses on one of the common fallacies in Western philosophy called the slippery slope. According to Patrick Hurley in A Concise Introduction to Logic, “the fallacy of slippery slope is a variety of the false cause fallacy. It occurs when the conclusion of an argument rests on an alleged chain reaction and there is not sufficient reason to think that the chain reaction will actually take place”. The key term in the chapter is “ (...) class='Hi'>chain reaction”. If all of the events in the chain reaction can be justified, then the slippery slope is not committed. But the nuances and intricate improbable connections between each event still make it difficult to support the ultimate conclusion, which is to get rid of cable. The chapter discusses two simple techniques in order to avoid committing the slippery slope fallacy. (shrink)

Scientific knowledge is based on the accumulation of investigations. The precise and exhaustive use of the sources and bibliography utilized in a investigation is the password wich decide the quality of the work, its success or its failure. Therefore, teledocumentation offers the adecuate support in arder to a fast, exhaustive, precise and pertinent access, in the moment that you want, to localize the necessary information and documentation for the development of sorne historical researchs.

In this essay, the articles which are published in the «Revista de Indias»,fiom 1995 to 1999, are analyzed under a scientometric perspective. The main purpose has been to obtain un intercontinental vision fkom the use of scient$c production in the subject of American History through a periodical publication which is rankedjirst among citations and the impact of its works. Firstly, citing works are studied: the numbers of published articles in the analyzed period, authors of fhese articles, and the institution of (...) the cited authors. Consecutively, citations are analyzed. Finally, we analyze the citation network that has been established between the American and European continents under the study of cited authors and the citing authors. (shrink)

La Cátedra de Historia Naval, impulsada por la Armada Española y la Universidad de Murcia, tiene entre sus objetivos investigar y difundir la Historia y el Patrimonio Naval y Marítimo. Con objeto acercar el conocimiento científico y la información divulgativa versada en esta materia al usuario, nuestra institución no se limita a utilizar los canales clásicos de comunicación como pueden ser los artículos y las monografías, entre otros, sino que, en su apuesta por las Humanidades Digitales, hace un uso pro-activo (...) de un blog y de unos perfiles en las redes sociales que, aparentemente, nos hacen contactar con el lector virtual de manera eficaz. Con el objetivo de seguir mejorando, a través del presente trabajo se analizan qué temas han suscitado un mayor interés entre los lectores de nuestras herramientas sociales, a través del estudio de las publicaciones de nuestra bitácora 2.0 y del reflejo de éstas en nuestra página en Facebook. La respuesta nos ayudará a conocer mejor los intereses y necesidades de información de los usuarios y, consecuentemente, podremos seguir perfeccionando los puentes digitales que hemos tendido entre ellos y la Historia y del Patrimonio Naval y Marítimo. (shrink)

In lieu of an abstract, here is a brief excerpt of the content:Philosophy and Rhetoric 35.2 (2002) 153-174 [Access article in PDF] LSDNA: Rhetoric, Consciousness Expansion, and the Emergence of Biotechnology Richard Doyle I had to struggle to speak intelligibly. —Albert Hofmann on his self-experiment with LSD-25 Finding a place to start is of utmost importance. Natural DNA is a tractless coil, like an unwound and tangled audio tape on the floor of the car in the dark. —Kary Mullis on (...) the invention of Polymerase Chain Reaction 1. Undoing life What happened is this: Once upon a time there was a narrative of vitality. Ok, not a narrative, but an apprehension, a fear, a vision. It didn't have a beginning, middle, or end. It was. Its contours were no more determinable than those of consciousness. Indeed, our consciousness, as humans, was our sole alleged difference from it. From something called "life." We were more than life, but were also, tragically, confined to it. You know the story all too well. Some of its major authors were Cuvier, Lamarck, Darwin, Bichat. Even Shelley.Just because this sudden transformation happened on paper doesn't mean it was easy to endure. Enormous numbers of humans went on tranquilizers just to deal with the effects. Others, elsewhere, knew nothing but rumors. Nonetheless, they became increasingly implicated in its effects, the effects of understanding and experiencing both "life" and "consciousness" as informatic events. Suddenly—and it is sudden, a real surprise—both our vitality and our thought were distributed, scattered across a network and nowhere in particular. Timothy Leary and Francis Crick were speaking the same language, the language of information where the organic and the machinic enfold each other helically and, sometimes, the capacity for replication goes through the ceiling. This new language of information would [End Page 153] introduce a novel response and abyss: the pleasures and hells of eternal replication. The distribution of both life and consciousness enabled a dream of immortality from which we have not yet awakened, as the mere specter of infinite clonal replicants provokes entropies of identity even as it preserves the self from onslaughts of decay. 1In at least one of its characteristics this shift toward an informatic understanding of life is perhaps not really so novel. The seductions of control are hardly, historians of science tell us, foreign to the practices and effects of technoscience. Evelyn Fox Keller makes this point concisely and precisely in her not-quite eponymous essay, "From Secrets of Life to Secrets of Death," itself a self-professed sequel to her 1986 "Making Gender Visible." Here the scientific "impulse" is underwritten by a perennial motif that underlies much of scientific creativity, namely, the urge to fathom the secrets of nature and the collateral hope that, in fathoming the secrets of nature, we will fathom (and hence gain control of) our own mortality (Secrets, 29).Surely Keller is correct that the nascent practices of molecular biology were cryptographic in character, an espionage project unknowingly launched by Erwin Schrodinger during the Second World War. Surely it is crucial to this moment that while Schrodinger's 1943 Dublin lectures were introducing audiences to the concept of the code-script, Alan Turing again brilliantly decrypted a modified U-boat code, giving the Allies code superiority for the remainder of the war. 2 But in this segment on that strange growth called "biotechnology," I want to focus on what Keller has called the "collateral" of hope in this cryptographic impulse, namely the collateral equation between inquiry and control, specifically the control of mortality.For is "fathoming" a secret identical to the exercise of control? The very cadence and rhetorical encryption of Keller's formulation here, a general parenthetical syllogistic conclusion within and alongside a hyphenated invocation of particularity, suggest that concepts and practices of secrecy are more unstable than Keller implies with her invocation of Feynman's "disease" of lock picking: "One of my diseases, one of my things in life, is that anything that is secret, I try to undo" (40).For Keller, Feynman's confession is emblematic of a desire to control life and death... (shrink)

Recognizing the profound need for greater patient and provider familiarity with personalized genomic medicine, many university instructors are including personalized genotyping as part of their curricula. During seminars and lectures students run polymerase chain reactions on their own DNA or evaluate their experiences using direct-to-consumer genetic testing services subsidized by the university. By testing for genes that may influence behavioral or health-related traits, however, such as alcohol tolerance and cancer susceptibility, certain universities have stirred debate on the ethical concerns (...) raised by educational genotyping. Considering the potential for psychosocial harm and medically relevant outcomes, how far should university-facilitated DNA testing be permitted to go? The analysis here distinguishes among these learning initiatives and critiques their approaches to the ethical concerns raised by educational genotyping. (shrink)

This book documents Szilard's energetic attempts to influence public policy on arms control and disarmament issues, both through open political processes and statements and through behindthe-scenes contacts with Washington power sources and a remarkable exercise in personal diplomacy with Nikita Khrushchev. Leo Szilard conceived of the possibility of nuclear fission sustained by a chain reaction years before it was achieved in the laboratory. He was also one of the initiators of the atomic bomb project in the United States. (...) Yet he dedicated his final years to the causes of understanding and sustaining life. The eminent physicist became a biologist and a vital force calling, for the control of nuclear and other weapons. This book documents Szilard's energetic attempts to influence public policy on arms control and disarmament issues, both through open political processes and statements and through behindthe-scenes contacts with Washington power sources and a remarkable exercise in personal diplomacy with Nikita Khrushchev. Many of the issues Szilard deals with in this valuable record of the years 1947-1963 are still crucial today. His opposition to antiballistic missile systems, his proposal for a Washington-Moscow "hot line," his work on the Pugwash conferences that brought together scientists from the East and the West, his pivotal role in the creation of the Council for a Livable World, his advocacy of a nuclear policy of no-first-use and restricted retaliation, and his support of "minimum deterrence" in place of an overwhelming counterforce capability - all these matters are as important in the 1980s as they were in the 1950s and 1960s. (shrink)

The ongoing coronavirus 2019 pandemic caused by the severe acute respiratory syndrome coronavirus 2 has resulted in a severe ramification on the global healthcare system, principally because of its easy transmission and the extended period of the virus survival on contaminated surfaces. With the advances in computer-aided diagnosis and artificial intelligence, this paper presents the application of deep learning and adversarial network for the automatic identification of COVID-19 pneumonia in computed tomography scans of the lungs. The complexity and time limitation (...) of the reverse transcription-polymerase chain reaction swab test makes it disadvantageous to depend solely on as COVID-19’s central diagnostic mechanism. Since CT imaging systems are of low cost and widely available, we demonstrate that the drawback of the RT-PCR can be alleviated with a faster, automated, and reduced contact diagnostic process via the use of a neural network model for the classification of infected and noninfected CT scans. In our proposed model, we explore the benefit of transfer learning as a means of resolving the problem of inadequate dataset and the importance of semisupervised generative adversarial network for the extraction of well-mapped features and generation of image data. Our experimental evaluation indicates that the proposed semisupervised model achieves reliable classification, taking advantage of the reflective loss distance between the real data sample space and the generated data. (shrink)

A crucial part of the knowledge of molecular biologists is procedural knowledge, that is, knowledge of how to do things in laboratories. Procedural knowledge of molecular biologists involves both perceptual-motor skills and cognitive skills. We discuss such skills required in performing the most commonly used molecular biology techniques, namely, Polymerase Chain Reaction and gel electrophoresis. We argue that procedural knowledge involved in performing these techniques is more than just knowing their protocols. Creative exploration and experience are essential for (...) the acquisition of procedural knowledge in molecular biology. With enough experience, molecular biologists make intuitive judgments without recourse to analytical reasoning. We propose that procedural knowledge is intuitive recognition of the patterns of one's environment that are the most relevant for making a decision or acting appropriately. Finally, we argue that knowledge of molecular biologists requires an integration of procedural knowledge and propositional knowledge. (shrink)

The assumption that all life on Earth today shares the same basic molecular architecture and biochemistry is part of the paradigm of modern biology. This paper argues that there is little theoretical or empirical support for this widely held assumption. Scientists know that life could have been at least modestly different at the molecular level and it is clear that alternative molecular building blocks for life were available on the early Earth. If the emergence of life is, like other natural (...) phenomena, highly probable given the right chemical and physical conditions then it seems likely that the early Earth hosted multiple origins of life, some of which produced chemical variations on life as we know it. While these points are often conceded, it is nevertheless maintained that any primitive alternatives to familiar life would have been eliminated long ago, either amalgamated into a single form of life through lateral gene transfer or alternatively out-competed by our putatively more evolutionarily robust form of life. Besides, the argument continues, if such life forms still existed, we surely would have encountered telling signs of them by now. These arguments do not hold up well under close scrutiny. They reflect a host of assumptions that are grounded in our experience with large multicellular organisms and, most importantly, do not apply to microbial forms of life, which cannot be easily studied without the aid of sophisticated technologies. Significantly, the most powerful molecular biology techniques available—polymerase chain reaction amplification of rRNA genes augmented by metagenomic analysis—could not detect such microbes if they existed. Given the profound philosophical and scientific importance that such a discovery would represent, a dedicated search for ‘shadow microbes’ seems in order. The best place to start such a search is with puzzling phenomena, such as desert varnish, that resist classification as ‘biological’ or ‘nonbiological’. (shrink)

Selected aneuploidies can be rapidly diagnosed by the analysis of fluorescent polymerase chain reaction (PCR) products of chromosome‐specific and highly polymorphic small tandem repeats (STRs). The quantitative STR patterns obtained from samples of normal individuals are markedly different from those seen when patients with aneuploidies involving chromosome X, or trisomies of chromosomes 21 and 18, are tested. For example, while samples from normal subjects – tested with a chromosome 21‐derived STR (D21S11) – show two fluorescent PCR peaks with (...) similar activities in a 1:1 ratio, the analysis of samples from patients with trisomy 21 reveals the presence of either three peaks (ratio 1:1:1), or two peaks with a ratio of 2:1. The use of an internal non‐polymorphic marker allows identification of trisomic samples with three copies of the same allele. This rapid approach (24 hours) is particularly valuable when applied to prenatal diagnosis of chromosomal abnormalities since it reduces the time of anxiety of the parents waiting for the results of the conventional cytogenetic tests, which require several weeks. (shrink)

The coronavirus disease 2019 pandemic has affected most countries of the world. The detection of Covid-19 positive cases is an important step to fight the pandemic and save human lives. The polymerase chain reaction test is the most used method to detect Covid-19 positive cases. Various molecular methods and serological methods have also been explored to detect Covid-19 positive cases. Machine learning algorithms have been applied to various kinds of datasets to predict Covid-19 positive cases. The machine learning (...) algorithms were applied on a Covid-19 dataset based on commonly taken laboratory tests to predict Covid-19 positive cases. These types of datasets are easy to collect. The paper investigates the application of decision tree ensembles which are accurate and robust to the selection of parameters. As there is an imbalance between the number of positive cases and the number of negative cases, decision tree ensembles developed for imbalanced datasets are applied. F-measure, precision, recall, area under the precision-recall curve, and area under the receiver operating characteristic curve are used to compare different decision tree ensembles. Different performance measures suggest that decision tree ensembles developed for imbalanced datasets perform better. Results also suggest that including age as a variable can improve the performance of various ensembles of decision trees. (shrink)

Current methods of forensic DNA profiling, based on Polymerase Chain Reaction amplifications of a varying number of Short Tandem Repeat loci found at different locations on the human genome, are regularly described as constituting the “gold standard for identification” in contemporary society. At a time when criminal justice systems in Europe and North America increasingly seek to utilize the epistemic authority of a variety of sciences in support of the apprehension and prosecution of suspects and offenders, genetic science (...) and recombinant DNA technology are often singled out for particular approbation. Indeed, the development and application of DNA profiling has been widely described as the “greatest breakthrough in forensic science since fingerprinting.”. (shrink)

The assumption that all life on Earth today shares the same basic molecular architecture and biochemistry is part of the paradigm of modern biology. This paper argues that there is little theoretical or empirical support for this widely held assumption. Scientists know that life could have been at least modestly different at the molecular level and it is clear that alternative molecular building blocks for life were available on the early Earth. If the emergence of life is, like other natural (...) phenomena, highly probable given the right chemical and physical conditions then it seems likely that the early Earth hosted multiple origins of life, some of which produced chemical variations on life as we know it. While these points are often conceded, it is nevertheless maintained that any primitive alternatives to familiar life would have been eliminated long ago, either amalgamated into a single form of life through lateral gene transfer (LGT) or alternatively out-competed by our putatively more evolutionarily robust form of life. Besides, the argument continues, if such life forms still existed, we surely would have encountered telling signs of them by now. These arguments do not hold up well under close scrutiny. They reflect a host of assumptions that are grounded in our experience with large multicellular organisms and, most importantly, do not apply to microbial forms of life, which cannot be easily studied without the aid of sophisticated technologies. Significantly, the most powerful molecular biology techniques available—polymerase chain reaction (PCR) amplification of rRNA genes augmented by metagenomic analysis—could not detect such microbes if they existed. Given the profound philosophical and scientific importance that such a discovery would represent, a dedicated search for ‘shadow microbes’ (heretofore unrecognized ‘alien’ forms of terran microbial life) seems in order. The best place to start such a search is with puzzling (anomalous) phenomena, such as desert varnish, that resist classification as ‘biological’ or ‘nonbiological’. Ó 2007 Elsevier Ltd.. (shrink)

In this chapter, typical examples of methodological individualism explanation are borrowed from Raymond Boudon’s writings. They respectively aim at answering the following questions:Why did Athens’ allies defect in the Peloponnesian War?When does social organization aim at eliminating unintended effects?Why does the rule of unanimity often prevail in traditional village societies?Why do members of an unorganized group tend to defect?Why are collective powers often governed by the iron law of oligarchy?Why did capitalist agriculture develop much more slowly in France than in (...) England in the eighteenth century?Why has the immoral character of interest lending disappeared in modern societies?Why is there no socialism in the United States?Why do economic booms seem to be associated with higher suicide rates?Why does the diffusion of an innovation follow a chain reaction process in situations where interpersonal influence is greater?Why were Mithra cult and Freemasonry, respectively, successful in Ancient Rome and modern Prussia?Why were the peasants in ancient Rome hostile to monotheism?Why did the French intellectuals of the late eighteenth century worship Reason?Why did Indian peasants not adopt the birth control measures advocated by the Indian administration?Why do conflicts between employees in a Taylorized firm tend to be more violent? (shrink)

In lieu of an abstract, here is a brief excerpt of the content:“We Need to Cut the Neck!”Confronting Psychological and Moral Distress during Emergency Cricothyrotomy1Stephanie CooperEnoughYou didn’t die in the ER, but rather, began your inexorable demise. The last, first, and only words I ever heard you utter was the weak mewl “tight, tight” as the blood pressure cuff constricted your left arm. You were 98–years–old, bed–bound, at the end. Your world was already partitioning itself from us, your brain tunneling (...) down the uneven corridors of consciousness. A concretion of otoliths muffled your hearing, and fatigue burdened your ancient heart.Your name–band encircled your skinny ankle like a bird. Deformed by arthritis, you were caged within yourself, imprisoned by senescence. You could not declare yourself to us. Words disintegrated into moans and your grimace became animal.When you came back from x–ray—hypotensive with multiple neck fractures confirmed—you could speak no more. Your pulse slowed, your blood pressure plummeted. I jammed a gloved finger against your femoral pulse, faint and tired. [End Page E5]“What’s her DNR status?” I gravely asked your nurse.The DNR paperwork for once had arrived with the patient. “No CPR” she said with relief. But then the paperwork went on, forcing me—the waitress of tragedy—to serve up the hash of an impossible menu.As health care providers, we are all often tasked with performing contradictory actions: the pain of IV insertion followed by the morphine push, drilling deeper into broken bone in order to plate it, the inadvertent cracking of ribs during chest compressions. We sometimes must perform violent action in the hope of sustaining and resurrecting life. We are asked to perform the impossible, the contradictory, the painful. And sometimes the actions we must take catalyze an inexorable chain reaction of actions, which can end in death–prolonging and painful interventions that we did not intend.Although our culture may imagine that one can choose one’s meal at the end–of–life fast–food franchise of death, such is not the case. You can’t suddenly decide you’re a vegetarian. You can’t order the cheeseburger without the cheese—and we all are getting the burger, in the end. Ordering the heart–squeezing drugs without the CPR to circulate them, or the antibiotics without the fluid to counter dehydration, is like ordering the burger without the bun. Sometimes the possible choices are not necessarily realistic choices. You get served what you get served, and perhaps we should question the assumption that we have the ability to choose at all.Your DNR form proclaimed no CPR but then said that antibiotics and intubation were okay—the cheeseburger without the cheese. An unfortunate choice for you, me, and the rest of us, as your death menu led to a very unhappy meal.You were comatose. I stuck a tongue blade deep into your desiccated throat. You did not gag. This means I should have intubated you, but morally, I couldn’t. To what end? You were 98 years old, broken, moribund. If you hadn’t suffered multiple neck fractures, or if you were a vibrant elder who had just walked off Mt. Rainier, maybe the story would have been different. But you? I doubted that you truly had a chance of ever feeling joy again. You were somebody’s wife, somebody’s mother. I knew this step would lead to the domino of unstoppable steps. I refused to flog your corpse.I called your daughter Kay on her cell phone, told her that you were in critical condition and that she should come to the ER immediately. But how to begin to tell a daughter her mother is dying, let alone over a cell phone? Gentle but surreal and impersonal, my voice tried to tell your daughter that the interventions you needed would likely kill you. I have seen enough human suffering to know. To me, the choices were clear: death by nature’s irrefutable course, versus a more painful, protracted, and violent death by what we’d do to you.Kay arrived in a wheelchair and scooted up to your bedside. Like all daughters, she wasn’t ready for you... (shrink)

We investigate the epistemological consequences of a positive polymerase chain reaction SARS-CoV test for two relevant hypotheses: V is the hypothesis that an individual has been infected with SARS-CoV-2; C is the hypothesis that SARS-CoV-2 is the cause of flu-like symptoms in a given patient. We ask two fundamental epistemological questions regarding each hypothesis: First, how much confirmation does a positive test lend to each hypothesis? Second, how much evidence does a positive test provide for each hypothesis against (...) its negation? We respond to each question within a formal Bayesian framework. We construe degree of confirmation as the difference between the posterior probability of the hypothesis and its prior, and the strength of evidence for a hypothesis against its alternative in terms of their likelihood ratio. We find that test specificity—and coinfection probabilities when making inferences about C—were key determinants of confirmation and evidence. Tests with 8) for V against ¬V regardless of sensitivity. Accordingly, low specificity tests could not provide strong evidence in favor of C in all plausible scenarios modeled. We also show how a positive influenza A test disconfirms C and provides weak evidence against C in dependence on the probability that the patient is influenza A infected given that his/her symptoms are not caused by SARS-CoV-2. Our analysis points out some caveats that should be considered when attributing symptoms or death of a positively tested patient to SARS-CoV-2. (shrink)

I have been involved with the current interest in de‐extinction since early 2012, nearly its beginning. I have given a lot of thought to the potential risks and benefits of de‐extinction. But only recently, after deep immersion in discussions around CRISPR‐Cas9, the hottest new tool in bioscience since polymerase chain reaction, have I thought about a more fundamental question: how, if at all, is de‐extinction special? Are “revived species” just another kind of genetically modified organism, raising essentially the (...) same general concerns? I answer, for the most part, yes. De‐extinction is not (very) special. New biotechnologies are giving humans even more power to change the biosphere but more directly, more quickly, and more utterly than ever before. De‐extinction is just one possible, and probably small, use of those technologies. Our attention, for the most part, should be on the bigger issues of regulating this power, rather than focusing specifically on their application to de‐extinction. (shrink)

The Earth agglomerates and heats. Convection cells within the planetary interior expedite the cooling process. Volcanoes evolve steam, carbon dioxide, sulfur dioxide and pyrophosphate. An acidulous Hadean ocean condenses from the carbon dioxide atmosphere. Dusts and stratospheric sulfurous smogs absorb a proportion of the Sun’s rays. The cooled ocean leaks into the stressed crust and also convects. High temperature acid springs, coupled to magmatic plumes and spreading centers, emit iron, manganese, zinc, cobalt and nickel ions to the ocean. Away from (...) the spreading centers cooler alkaline spring waters emanate from the ocean floor. These bear hydrogen, formate, ammonia, hydrosulfide and minor methane thiol. The thermal potential begins to be dissipated but the chemical potential is dammed. The exhaling alkaline solutions are frustrated in their further attempt to mix thoroughly with their oceanic source by the spontaneous precipitation of biomorphic barriers of colloidal iron compounds and other minerals. It is here we surmise that organic molecules are synthesized, filtered, concentrated and adsorbed, while acetate and methane—separate products of the precursor to the reductive acetyl-coenzyme-A pathway—are exhaled as waste. Reactions in mineral compartments produce acetate, amino acids, and the components of nucleosides. Short peptides, condensed from the simple amino acids, sequester ‘ready-made’ iron sulfide clusters to form protoferredoxins, and also bind phosphates. Nucleotides are assembled from amino acids, simple phosphates carbon dioxide and ribose phosphate upon nanocrystalline mineral surfaces. The side chains of particular amino acids register to fitting nucleotide triplet clefts. Keyed in, the amino acids are polymerized, through acid–base catalysis, to alpha chains. Peptides, the tenuous outer-most filaments of the nanocrysts, continually peel away from bound RNA. The polymers are concentrated at cooler regions of the mineral compartments through thermophoresis. RNA is reproduced through a convective polymerase chain reaction operating between 40 and 100°C. The coded peptides produce true ferredoxins, the ubiquitous proteins with the longest evolutionary pedigree. They take over the role of catalyst and electron transfer agent from the iron sulfides. Other iron–nickel sulfide clusters, sequestered now by cysteine residues as CO-dehydrogenase and acetyl-coenzyme-A synthase, promote further chemosynthesis and support the hatchery—the electrochemical reactor—from which they sprang. Reactions and interactions fall into step as further pathways are negotiated. This hydrothermal circuitry offers a continuous supply of material and chemical energy, as well as electricity and proticity at a potential appropriate for the onset of life in the dark, a rapidly emerging kinetic structure born to persist, evolve and generate entropy while the sun shines. (shrink)

The nuclear engineer emerged as a new form of recognised technical professional between 1940 and the early 1960s as nuclear fission, the chain reaction and their applications were explored. The institutionalization of nuclear engineering channelled into new national laboratories and corporate design offices during the decade after the war, and hurried into academic venues thereafter proved unusually dependent on government definition and support. This paper contrasts the distinct histories of the new discipline in the USA and UK (and, (...) more briefly, Canada). In the segregated and influential environments of institutional laboratories and factories, historical actors such as physicist Walter Zinn in the USA and industrial chemist Christopher Hinton in the UK proved influential in shaping the roles and perceptions of nuclear specialists. More broadly, I argue that the State-managed implantation of the new subject within further and higher education curricula was shaped strongly by distinct political and economic contexts in which secrecy, postwar prestige and differing industrial cultures were decisive factors. (shrink)

The explosion of the first atomic bomb at the Alamagordo Bombing Range, New Mexico, on July 16, 1945, was the start of a new era, the dawn of the atomic age. The preliminary work on atomic fission was done before the last world war, and as military leaders visioned its great destructive possibilities, several countries entered the race to be the first to harness atomic fission for military purposes. Our own country gambled two billion dollars, and with the aid of (...) certain European scientists and in collaboration with England and Canada, succeeded in developing a fission chain reaction that made possible the atomic bomb. (shrink)

Purpose: This study is aimed to preliminary investigate whether transcranial alternating current stimulation could affect explosive power considering genetic background in sport subjects.Methods: Seventeen healthy sports volunteers with at least 3 years of sports activities participated in the experiment. After 2 weeks of familiarization performed without any stimulation, each participant received either 50 Hz-tACS or sham-tACS. Before and after stimulation, subjects performed the following tests: the squat jump with the hands on the hips ; countermovement jump with the hands on (...) the hips ; countermovement jump with arm swing ; 15-s Bosco’s test; seated backward overhead medicine ball throw ; seated chest pass throw with a 3-kg rubber medicine ball; and hand-grip test. Additionally, saliva samples were collected from each participant. Genotyping analysis was carried out by polymerase chain reaction.Results: No significant differences were found in sport performance of subjects after 50 Hz-tACS. Additionally, we did not find any influence of genetic background on tACS-related effect on physical performance. These results suggest that tACS at gamma frequency is not able to induce an after-effect modulating sport performance. Further investigations with larger sample size are needed in order to understand the potential role of non-invasive brain stimulation techniques in motor performances.Conclusions: Gamma-tACS applied before the physical performance fails to improve explosive power in sport subjects. (shrink)

Section I explores and articulates Beccaria's theory of luxury. Social classes tend to emulate the classes immediately above and below them. When a class increases the luxury that it consumes, this causes a chain reaction of increased demand for luxury by other classes. Satisfying the resulting new demand for luxury and non-luxury goods maximizes the happiness of a greater number of citizens. Following the consequentialist principle of utility theory, Beccaria concludes that luxury is beneficial. His writings are compared (...) to those of Hume, Bentham and others. In section II Beccaria's demonstration that freedom of choice is a universally desired luxury indispensable for well-being in all human societies is contrasted to Rousseau's belief that luxury diminishes liberty and happiness. Beccaria values the distributive more than the aggregative maximization of utility in his analysis of luxury; the article explains why this carries wider implications for utilitarian theory. (shrink)

In environmental ethics a debate has arisen over the extent to which the individual should make changes in personal lifestyle in a long-term program of ameliorating environmental degradation, as opposed to directing energies toward public-policy change. In opposition are the facts that an individual’s contribution to environmental degradation can only have a negligible effect. Public policy offers the only real hope for such massive coordinated effort, and environmental degradation is only one of many global problems to which ethi­cally oriented people (...) must focus their attentions and energies. So far, the pro-personal responsibility side has urged that personal lifestyle changes are necessary for moral coher­ence, thus in turn for integrity of character, and lifestyle changes can affect others in a kind of chain reaction because humans are socially submerged beings. The stalemate here can only stymies the needed coordinated effort toward ameliorating environmental degradation. Further, moral concerns need to be brought into this issue; namely, the ramifications of pursuing a policy-only approach, emphasizing policy as the sole (or even primary) means of ameliorating environmental degradation, implicitly undercuts the role of individual agents in morality in general, in terms of (1) individual responsibility, (2) autonomy, and (3) creativity in solving problems. All these problems not only bear on the program to reverse environmental degradation, but undermine other widely held moral values. Emphasizing personal lifestyle responsibility is not only the most moral alternative but is also the most assured way to affect long-term changes and the better way to make policy changes credible and sufficiently substantive for change. (shrink)

IntroductionStress is associated with disease and reduced leukocyte telomere length. The objective of this research is to determine if self-perceived stress is associated with telomere length in Costa Rican adults and the gender differences in this association. Findings may help explain how some populations in apparent socioeconomic disadvantage and with limited access to specialized medical services have a remarkably high life expectancy.MethodologyData come from the pre-retirement cohort of the Costa Rican Longevity and Healthy Aging Study, a population based survey conducted (...) in the households to 2,327 adults aged 53 to 66 years. The DNA to measure LTL was extracted from blood cells in laboratories of the University of Costa Rica whereas the Blackburn laboratory at the University of California performed the telomere length measurement applying the quantitative polymerase chain reaction. The relationship between telomere length and perceived stress was measured using least-squares multiple regression. Perceived stress was measured by a set of questions about family, job, finances and, health reasons to be stressed. Models included the control variables: age and sex of the participant, whether he or she resides in the Nicoya area, a “blue zone” known for its high longevity, and the aforementioned sociodemographic, health and lifestyles characteristics.ResultsStress perception and LTL are significantly different by sex. Women perceived higher stress levels than men in almost all aspects studied, except work. Women have significantly longer telomeres. Shorter telomeres are significantly associated with caregiving stress in men and with parental health concerns in women. Counter-intuitive telomere lengthenings were observed among women who feel stressed about caring for family members; and among men who feel stressed due to their family relationships as well as concerns about their own health.DiscussionResults confirm that people with self-perceived stress due to caregiving or health issues have shorter telomeres. The relationship between stress and telomere length differs between men and women. Gender relations exert a strong modifier effect on the relationship between stress and LTL: gender is related to perceived stress, telomere length, and apparently also to the way stress and LTL are related. (shrink)

Just like atomic physics seventy years ago, when it was realized that chain reaction could lead to medical applications as well as to the creation of atomic weapons, the life sciences have entered a grey zone. “Advances in biotechnology […]” a 2003 CIA document stated, “have the potential to create a much more dangerous biological warfare threat […] Engineered biological agents could be much worse than any disease known to man”. As sociologists of science have noted, contemporary life (...) sciences have dual use potential as they can be used to foster beneficial applications, for instance in health care, or generate harm, either intentionally or not, and possibly on an unprecedented.. (shrink)

Due to the COVID-19 outbreak, a series of chain reactions, like international trade breakdown, stock market collapse, and crude oil's collapse, have adversely affected the global economy, particularly small and medium-sized enterprises. As a result, the Chinese government issued many fiscal and financial policies to support SMEs. This paper analyzes SMEs' coping methods and conceptual changes affected by the epidemic and distinguishes “victims” and “beneficiaries.” Subsequently, based on extensive international experience and local government experience, it provides effective suggestions for (...) the Chinese government to deal with the post-epidemic era's economic changes, policy suggestions, and coping strategies for SMEs' short-term and long-term sustainable development. (shrink)

Mass testing is one COVID-19 pandemic response strategy. The effect of population-wide testing programs is influenced by public attitudes toward COVID-19 viral tests. However, the public’s attitudes toward mass testing and related factors in mainland China are not adequately understood. This study focuses on pandemic responses during the first wave of the Delta variant outbreak in southern China and explores how residents responded to population-wide mass COVID-19 testing programs. The research relies on data collected from short videos recording residents’ experiences (...) of being in lockdown, media reports, and semi-structured interviews. Thematic analysis was used to analyze the data, and four themes emerged: public attitudes toward mass testing, the technology of viral tests, policy and governance, and cultural practices. The study finds that residents actively participated in mass testing campaigns as mass viral tests are associated with hope and trust. The Reverse Transcription–Polymerase Chain Reaction (RT-PCR), negative test results, lockdown policies, waiting times, medical staff, and media representations are all actors that assemble and mobilize hope and trust. The research reveals some critical factors influencing people’s attitudes toward mass testing policies in response to COVID-19 and provides practical suggestions for public health professionals in rolling out effective population-wide mass COVID-19 testing. (shrink)

The advances in treatment of HIV and the introduction of polymerase chain reaction assay for the virus now make it acceptable for HIV discordant couples where the male partner is seropositive to attempt to conceive through artificial insemination by husband or via in vitro fertilisation. With undetectable viral load and washed sperm, there is minimal risk of transmission of HIV to the female partner, children, other patients, or staff. We describe the development of a programme of AIH for (...) HIV discordant couples and the reasoning behind offering such a programme. (shrink)

The rapid sequencing of entire genomes based in large measure on a DNA cloning procedure, the polymerase chain reaction (PCR), has opened new frontiers in the discovery process for novel therapeutic agents. DNA cloning is a basic tool in genomics and it has been used for over a decade. Drug discovery is currently focused on the identification of gene databases, gene arrays and protein arrays aimed at therapeutic modulation of disease-related genes—which require procedures that may involve cloning techniques. (...) Currently, cloning for pharmacologic products is most applicable in the preclinical area of drug discovery. For example, the identification of specific receptor subtypes occurred via cloning whereas traditional pharmacologic approaches that use agonists and antagonists may have failed to separate binding affinities. Successful therapeutic agents where cloning played vital role resulted in the development of synthetic insulin, growth hormone, erythropoiten and other tissue factors in blood products, Vaccines may be another successful application derived from cloning. Another area where cloning can make a significant impact upon human therapeutics is that this technique may provide a new source of cells for tissue engineering and transplantation. Stem cells, and in particular, embryonic stem cells can differentiate into other cell types such as hepatocytes and neurons to provide normal products for hepatic and neurological diseases. However, the use of cloning embryonic stem cells introduces important ethical, medical and legal issues which require that leaders in government, academic, and religious groups be fully educated in these applications. A close working relationship among these diverse groups is needed to respond wisely to the introduction of new therapeutic opportunities while at the same time providing protection for the individual and the public at large from unethical practises. (shrink)

The authors of the article are trying to identify the signs of a crisis of our time as a consequence of the refusal from the philosophical justification of the goals and objectives of scientific pedagogy, which as a chain reaction entail ignoring the results of sciences related to pedagogy: neurobiology and psychology. The reproductive approach in education selectively takes into account the results of the empirical sciences, as they serve to justify it. The result is a shift in (...) emphasis: being driven by the hypothetical imperatives of competitiveness in the labor market, teachers and other adults feel the need for the achievements of the pupils, for their effectiveness. As a consequence, the child's psyche does not develop properly which manifests itself in the inability to see the subjects of interaction in the vis-a-vis, in an effort to objectify what is not subject to objectification - a person. The authors of the article see the solution to the problem in the appeal in the philosophical and pedagogical system of I. Kant, that may be reconstructed from his works. The authors of the article conclude that I. Kant may rightfully be considered the founder of a productive approach to education. (shrink)